Qiwen Zhong

Different soil nutrients affect the accumulation characteristics of plant metabolites. To investigate the differences among the metabolites of pepino grown in greenhouses on the Loess Plateau in northwest China, we investigated the main soil nutrients and their correlation with metabolites. A total of 269 pepino metabolites were identified using UPLC-QTOF-MS to detect metabolites in fruits from three major pepino growing regions and analyze their differential distribution characteristics. A total of 99 of these substances differed among pepino fruits from the three areas, and the main classes of the differential metabolites were, in order of number: amino acids and derivatives, nucleotides and derivatives, organic acids, alkaloids, vitamins, saccharides and alcohols, phenolic acids, lipids and others. An environmental factor analysis identified soil nutrients as the most significant differentiator. Five soil nutrient indicators: TN (total nitrogen), TP (total phosphorus), AP (available phosphorus), AK (available potassium), and OM (organic matter), exhibited significant differences in three growing sites. Metabolite and soil nutrient association analysis using redundancy analysis (RDA) and the Mantel test indicated that TN and OM contributed to the accumulation of amino acids and derivatives, nucleotides and derivatives, and alkaloids while inhibiting organic acids, vitamins coagulation biosynthesis. Moreover, AP and TP were associated with the highest accumulation of saccharides and, alcohols, phenolic acids. Consequently, differences in soil nutrients were reflected in pepino metabolite variability. This study clarified the metabolite variability and the relationship between pepino and soil nutrients in the main planting areas of northwest China. It provides a theoretical basis for the subsequent development of Pepino’s nutritional value and cultivation management.

In order to fully understand and effectively utilize the genetic diversity of Jerusalem artichoke accessions in China. 257 Jerusalem artichoke accessions were analyzed. Among them, 12 quantitative traits had 6%–50% of the coefficient of variation, with a mean of 24.75%, which was the highest in tuber weight per plant (50%) and the smallest in growth period (6%). The diversity index (H′) of these 12 traits was 1.24–1.53, with a mean of 1.44, which was the highest in tuber number per plant (1.53) and the lowest in leaf width (1.24). The diversity index of the eight quality traits was 0.85–1.08, with a mean of 0.98, which was the highest in tuber habit and the lowest in tuber uniformity, showing rich genetic diversity in most traits. The subordinate function of 257 Jerusalem artichoke accessions was 0.12–0.58, in which the highest was in JA1095 (0.58) with the obvious advantages of flower number and tuber weight per plant. The correlation analysis of 12 quantitative traits indicated that stem diameter, leaf length, flower and disk size could be used as the main target traits for high yield Jerusalem artichoke varieties breeding in the future. The results of principal component analysis showed that the cumulative contribution rate of the seven principal component factors was 66.794%. Among them, the number of flowers, the tuber number per plant, the number of tuber hairs and the smoothness of tuber epidermis were the main factors contributing to the phenotypic difference of Jerusalem artichoke. By cluster analysis, 257 accessions materials were divided into five categories based on 20 traits,among them class I and class II accounted for 85% of the total germplasm resources. This results can provide an important reference for the utilization of Jerusalem artichoke accessions and variety breeding.

Dormancy-associated MADS-box (DAM) genes play an important role in plant dormancy and release phases. Little is known about the dormancy characteristics of Jerusalem artichoke tubers. Using bioinformatics, we identified and annotated 23 MADS-box gene sequences from the genome of the Jerusalem artichoke and we analyzed the differential expression of these genes at different developmental stages of tuber dormancy. The results show that all 23 genes encode basic proteins and most of the genes of the same subgroup have similar pI values. MADS-box genes from the Jerusalem artichoke and from other closely related species were divided into ten categories using phylogenetic analysis software. Based on the amino acid sequence of the MADS-domain proteins, ten highly conserved motifs were identified. Gene ontology annotation, InterProScan protein function prediction, and RT-PCR analysis showed that ten MADS-box genes play important roles in the dormancy process of Jerusalem artichoke tubers. Our work lays a foundation for further study of the role of MADS-box genes in the dormancy of the Jerusalem artichoke and other tuber crops.

Jerusalem artichoke (Helianthus tuberosus L.) is widely cultivated in Northwest China, and it has become an emerging economic crop that is rapidly developing. Because of its elevated inulin content and high resistance, it is widely used in functional food, inulin processing, feed, and ecological management. In this study, Illumina sequencing technology was utilized to assemble and annotate the complete chloroplast genome sequences of Jerusalem artichoke. The total length was 151,431 bp, including four conserved regions: A pair of reverse repeat regions (IRa 24,568 bp and IRb 24,603 bp), a large single-copy region (83,981 bp), and a small single-copy region (18,279 bp). The genome had a total of 115 genes, with 19 present in the reverse direction in the IR region. A total of 36 simple sequence repeats (SSRs) were identified in the coding and non-coding regions, most of which were biased toward A/T bases. A total of 32 SSRs were distributed in the non-coding regions. A comparative analysis of the chloroplast genome sequence of the Jerusalem artichoke and other species of the composite family revealed that the chloroplast genome sequences of plants of the composite family were highly conserved. Differences were observed in 24 gene loci in the coding region, with the degree of differentiation of the ycf2 gene being the most obvious. A phylogenetic analysis showed that H. petiolaris subsp. fallax had the closest relationship with Jerusalem artichoke, both members of the Helianthus genus. Selective locus detection of the ycf2 gene in eight species of the composite family was performed to explore adaptive evolution traits of the ycf2 gene in Jerusalem artichoke. The results show that there are significant and extremely significant positive selection sites at the 1239N and 1518R loci, respectively, indicating that the ycf2 gene has been subject to adaptive evolution. Insights from our assessment of the complete chloroplast genome sequences of Jerusalem artichoke will aid in the in-depth study of the evolutionary relationship of the composite family and provide significant sequencing information for the genetic improvement of Jerusalem artichoke.

Chicory (Cichorium intybus L.) root is the main raw material used for inulin production, and chicory leaf represents an important forage resource. This is the first reported sequence for a chloroplast genome of the chicory family Compositae. The length of the intact chicory chloroplast genome was 152,975 bp, consisting of one reverse repeat region (IRs, 50,038 bp), one small single-copy region (SSC, 18,561 bp), and one large single-copy region (LSC, 84,376 bp). The chicory chloroplast genome contains 127 functional genes, including 74 protein-coding genes, 29 tRNA genes, and 24 rRNA genes. An analysis of the phylogenetic relationship of chicory to other species revealed that chicory is closely related to the Chrysanthemum and Artemisia groups. Together these results serve to lay a foundation for the ongoing evolutionary and ecological study of Cichorium.

In recent years, Jerusalem artichoke has received widespread attention as a novel source of sugar, biofuel, and animal feed. Currently, only few gDNA-SSRs derived from sunflower were verified in the Jerusalem artichoke; therefore, it is particularly important to develop SSR primer markers that belonged to Jerusalem artichoke resources. Using EST data to develop EST-SSR markers is simple and effective. In order to understand the general characteristics of SSR markers in Jerusalem artichoke EST sequences and accelerate the use of SSR markers in Jerusalem artichoke research. This study used 40,370 sequenced unigene fragments and MISA software to identify SSR loci. The 48 pairs of EST-SSR primers assessed for the identification of 45 varieties of Jerusalem artichoke. Cluster, genetic diversity parameters and AMOVA analysis was conducted using the genetic similarity coefficient, revealing genetic differences between 48 genetic material. A total of 1204 SSR loci were identified with 13 different types of repeats, distributed among 1020 EST sequences, of which trinucleotide repeats were the most common, accounting for 38.21% of the total SSR loci. Among the 44 repeat motifs, AG/CT, AAG/CTT, and ATC/ATG motifs had the highest frequencies, accounting for 22.45, 14.71, and 7.84% of all motifs, respectively. From these sequences, 48 pairs of EST-SSR primers were designed, and 22 primer pairs for loci with high polymorphism were selected to analyze the genetic diversity of 45 Jerusalem artichoke germplasm sources. The results indicated that the variation range of the effective number of alleles for 22 primers ranged between 1.7502 and 4.5660. The Shannon’s information index ranged between 0.6200 and 1.6423. The variation range of PIC ranged between 0.3121 and 0.6662 with an average of 0.5184. Cluster analysis was conducted using the genetic similarity coefficient, revealing significant genetic differences between Asian and European genetic material. Cluster analysis revealed a relationship between the genotypes and geographic origins of the Jerusalem artichoke. The results of AMOVA as well as the genetic identity and genetic distance in the Jerusalem artichoke population showed that there presented certain genetic heterogeneity in Jerusalem artichoke genetic structure of 45 samples from seven different geographic populations. The Jerusalem artichoke EST-SSR marker system established in this study provides an effective molecular marker system for future research focused on Jerusalem artichoke genetic diversity and the breeding of new varieties.