Mahsa Karbaschi
Research Subjects
Mahsa Karbaschi has a total of 9 co-authors. Here are the top co-authors by number of shared publications:
- Apurva Sonawane
Florida International University
1 shared publication - Chris Ross
Engineering Resources Group, Pembroke Pines, FL 33029, USA
1 shared publication - J. Sebastian Marquez
Department of Electrical and Computer Engineering, Florida International University, Miami, FL, United States
1 shared publication - Marcus S. Cooke
Oxidative Stress Group, Department of Molecular Biosciences, University of South Florida, Tampa, FL 33620, USA
1 shared publication - Mario Mendoza
Department of Electrical and Computer Engineering, Florida International University, Miami, FL 33174, USA
1 shared publication - Mubarak A. Mujawar
Florida International University
1 shared publication
Latest Publications
- Open Access
- 13 April 2023
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 24 (8) , 7187
Development of a Novel, Automated High-Throughput Device for Performing the Comet Assay
A comet assay is a trusted and widely used method for assessing DNA damage in individual eukaryotic cells. However, it is time-consuming and requires extensive monitoring and sample manipulation by the user. This limits the throughput of the assay, increases the risk of errors, and contributes to intra- and inter-laboratory variability. Here, we describe the development of a device which automates high throughput sample processing for a comet assay. This device is based upon our patented, high throughput, vertical comet assay electrophoresis tank, and incorporates our novel, patented combination of assay fluidics, temperature control, and a sliding electrophoresis tank to facilitate sample loading and removal. Additionally, we demonstrated that the automated device performs at least as well as our “manual” high throughput system, but with all the advantages of a fully “walkaway” device, such as a decreased need for human involvement and a decreased assay run time. Our automated device represents a valuable, high throughput approach for reliably assessing DNA damage with the minimal operator involvement, particularly if combined with the automated analysis of comets.
- 13 April 2023
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 24 (8) , 7187
Open AccessDevelopment of a Novel, Automated High-Throughput Device for Performing the Comet Assay
A comet assay is a trusted and widely used method for assessing DNA damage in individual eukaryotic cells. However, it is time-consuming and requires extensive monitoring and sample manipulation by the user. This limits the throughput of the assay, increases the risk of errors, and contributes to intra- and inter-laboratory variability. Here, we describe the development of a device which automates high throughput sample processing for a comet assay. This device is based upon our patented, high throughput, vertical comet assay electrophoresis tank, and incorporates our novel, patented combination of assay fluidics, temperature control, and a sliding electrophoresis tank to facilitate sample loading and removal. Additionally, we demonstrated that the automated device performs at least as well as our “manual” high throughput system, but with all the advantages of a fully “walkaway” device, such as a decreased need for human involvement and a decreased assay run time. Our automated device represents a valuable, high throughput approach for reliably assessing DNA damage with the minimal operator involvement, particularly if combined with the automated analysis of comets.