Ernesto Mondol
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Research Interests
Ernesto Mondol has a total of 9 co-authors. Here are the top co-authors by number of shared publications:
- Ana Carolina Mercado
Institute for Immunological Research, University of Cartagena, Cartagena 130014, Colombia
1 shared publication - Dilia Mercado
Institute for Immunological Research, University of Cartagena, Cartagena 130014, Colombia
1 shared publication - Ines Benedetti
Department of Basic Sciences, School of Medicine, University of Cartagena, Cartagena 130014, Colombia
1 shared publication - Josefina Zakzuk
Institute for Immunological Research, University of Cartagena, Cartagena 130014, Colombia
1 shared publication - Karen Donado
Institute for Immunological Research, University of Cartagena, Cartagena 130014, Colombia
1 shared publication - Karen Hernandez
Institute for Immunological Research, University of Cartagena, Cartagena 130014, Colombia
1 shared publication
Latest Publications
- Open Access
- 14 March 2023
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 24 (6) , 5543
The Allergenic Activity of Blo t 2, a Blomia tropicalis IgE-Binding Molecule
Only few allergens derived from house dust mite (HDM) species have been evaluated in terms of their potential to induce allergic inflammation. In this study, we aimed to evaluate different aspects of the allergenicity and allergenic activity of Blo t 2, a Blomia tropicalis allergen. Blo t 2 was produced as a recombinant protein in Escherichia coli. Its allergenic activity was tested in humans by skin prick test and basophil activation assays, and in mice, by passive cutaneous anaphylaxis and a model of allergic airway inflammation. Sensitization rate to Blo t 2 (54.3%) was similar to that found to Blo t 21 (57.2%) and higher than to Der p 2 (37.5%). Most Blo t 2-sensitized patients showed a low intensity response (99.5%). Blo t 2 elicited CD203c upregulation and allergen induced skin inflammation. Additionally, immunized animals produced anti-Blo t 2 IgE antibodies and passive transfer of their serum to non-immunized animals induced skin inflammation after allergen exposure. Immunized animals developed bronchial hyperreactivity and a strong inflammatory lung reaction (eosinophils and neutrophils). These results confirm the allergenic activity of Blo t 2 and supports its clinical relevance.
- 14 March 2023
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 24 (6) , 5543
Open AccessThe Allergenic Activity of Blo t 2, a Blomia tropicalis IgE-Binding Molecule
Only few allergens derived from house dust mite (HDM) species have been evaluated in terms of their potential to induce allergic inflammation. In this study, we aimed to evaluate different aspects of the allergenicity and allergenic activity of Blo t 2, a Blomia tropicalis allergen. Blo t 2 was produced as a recombinant protein in Escherichia coli. Its allergenic activity was tested in humans by skin prick test and basophil activation assays, and in mice, by passive cutaneous anaphylaxis and a model of allergic airway inflammation. Sensitization rate to Blo t 2 (54.3%) was similar to that found to Blo t 21 (57.2%) and higher than to Der p 2 (37.5%). Most Blo t 2-sensitized patients showed a low intensity response (99.5%). Blo t 2 elicited CD203c upregulation and allergen induced skin inflammation. Additionally, immunized animals produced anti-Blo t 2 IgE antibodies and passive transfer of their serum to non-immunized animals induced skin inflammation after allergen exposure. Immunized animals developed bronchial hyperreactivity and a strong inflammatory lung reaction (eosinophils and neutrophils). These results confirm the allergenic activity of Blo t 2 and supports its clinical relevance.