Elise Neubarth
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Elise Neubarth has a total of 6 co-authors. Here are the top co-authors by number of shared publications:
- Amy Makler
Asghar-Lab—Micro and Nanotechnology in Medicine Lab, Florida Atlantic University, Boca Raton, FL 33431, USA
1 shared publication - Mazhar Sher
Department of Agricultural and Biosystems Engineering, South Dakota State University, Brookings, USA
1 shared publication - Natasha Ramnauth
Asghar-Lab—Micro and Nanotechnology in Medicine Lab, Florida Atlantic University, Boca Raton, FL 33431, USA
1 shared publication - Steven Soini
Department of Chemistry and Biochemistry, Department of Ocean and Mechanical Engineering, Florida Atlantic University, Boca Raton, FL 33431, USA
1 shared publication - Vivian Merk
Florida Atlantic University
1 shared publication - Waseem Asghar
Asghar-Lab, Micro and Nanotechnology in Medicine, College of Engineering and Computer Science, Florida Atlantic University, Boca Raton, FL 33431, USA
1 shared publication
Latest Publications
- Open Access
Development of a Microfluidic Device for Exosome Isolation in Point-of-Care Settings
Exosomes have gained recognition in cancer diagnostics and therapeutics. However, most exosome isolation methods are time-consuming, costly, and require bulky equipment, rendering them unsuitable for point-of-care (POC) settings. Microfluidics can be the key to solving these challenges. Here, we present a double filtration microfluidic device that can rapidly isolate exosomes via size-exclusion principles in POC settings. The device can efficiently isolate exosomes from 50–100 µL of plasma within 50 min. The device was compared against an already established exosome isolation method, polyethylene glycol (PEG)-based precipitation. The findings showed that both methods yield comparable exosome sizes and purity; however, exosomes isolated from the device exhibited an earlier miRNA detection compared to exosomes obtained from the PEG-based isolation. A comparative analysis of exosomes collected from membrane filters with 15 nm and 30 nm pore sizes showed a similarity in exosome size and miRNA detection, with significantly increased sample purity. Finally, TEM images were taken to analyze how the developed devices and PEG-based isolation alter exosome morphology and to analyze exosome sizes. This developed microfluidic device is cost-efficient and time-efficient. Thus, it is ideal for use in low-resourced and POC settings to aid in cancer and disease diagnostics and therapeutics.
Open AccessDevelopment of a Microfluidic Device for Exosome Isolation in Point-of-Care Settings
Exosomes have gained recognition in cancer diagnostics and therapeutics. However, most exosome isolation methods are time-consuming, costly, and require bulky equipment, rendering them unsuitable for point-of-care (POC) settings. Microfluidics can be the key to solving these challenges. Here, we present a double filtration microfluidic device that can rapidly isolate exosomes via size-exclusion principles in POC settings. The device can efficiently isolate exosomes from 50–100 µL of plasma within 50 min. The device was compared against an already established exosome isolation method, polyethylene glycol (PEG)-based precipitation. The findings showed that both methods yield comparable exosome sizes and purity; however, exosomes isolated from the device exhibited an earlier miRNA detection compared to exosomes obtained from the PEG-based isolation. A comparative analysis of exosomes collected from membrane filters with 15 nm and 30 nm pore sizes showed a similarity in exosome size and miRNA detection, with significantly increased sample purity. Finally, TEM images were taken to analyze how the developed devices and PEG-based isolation alter exosome morphology and to analyze exosome sizes. This developed microfluidic device is cost-efficient and time-efficient. Thus, it is ideal for use in low-resourced and POC settings to aid in cancer and disease diagnostics and therapeutics.