Hichem Sebai

BisphenolA (BPA) is a common endocrine disruptor that impairs male fertility through oxidative stress and alterations in membrane lipids. This study evaluated the protective effects of Myrtus communis L. essential oil (EOMC) on BPA-induced sperm toxicity in Wistar rats in vitro. BPA significantly decreased sperm motility and viability. It also increased lipid peroxidation, depleted thiols, and reduced the activity of antioxidant enzymes (SOD, CAT-like and GPx-like). Concomitant treatment with low and intermediate doses of EOMC (0.5–1 µL/mL) restored sperm function, reduced oxidative stress, and preserved membrane phospholipids. However, the highest dose (5 µL/mL) further impaired sperm function and disrupted membrane phospholipids. BPA also altered amino acid profiles and accumulated intracellularly, effects partially reversed by EOMC, which redistributed free BPA into the culture medium. Bioavailability analysis revealed selective absorption of α-pinene, while d-limonene and 1,8-cineole were undetectable. Molecular modeling indicated strong binding of BPA to antioxidant enzymes, potentially disrupting their structure and activity. Overall, these results show that EOMC protects sperm from BPA-induced damage in a dose-dependent manner through antioxidant, membrane-stabilizing, and redistribution mechanisms. This highlights its potential application in phytotherapy for male reproductive health.

Urtica dioica L. has been used as a natural remedy due to its healing properties for over 2000 years. The aim of this study is to investigate the chemical composition, antimicrobial, antioxidant, and antitumor properties in vitro of the aqueous extract of Urtica dioica leaves (AEUD). The chemical composition was assessed by an ultra-high-performance liquid chromatography system coupled to a benchtop QExactive high-resolution accurate mass spectrometry operating in a data-dependent acquisition mode as a non-target approach. Minimal inhibitory concentration (MIC) and disc diffusion were used to assess the antibacterial efficacy against nine bacterial strains. The antioxidant impact was assessed using DPPH, ABTS, FRAP, and ferrous ion-chelating ability assays. By using the MTT method, the cytotoxicity effect of AEUD on colon cancer cell HCT-116 was evaluated. Flow cytometry was used to analyze the cell cycle. Finally, the anti-migration and anti-invasion properties of AEUD on HCT-116 cells were estimated using the wound healing test and Transwell assays. AEUD is a rich source of phenolic compounds. The results of disc diffusion and MIC showed that the AEUD is more active against Gram-positive bacteria than against Gram-negative bacteria. MTT assay confirmed that the AEUD inhibited HCT-116 colon cancer cell proliferation. Findings of flow cytometry confirmed that cell cycle arrest occurred at the G2 phase. Additionally, AEUD had anti-migration and anti-invasion effects. This study shows that Urtica dioica aqueous leaf extract exhibits potential antibacterial, antioxidant, and antitumoral activities on HCT-116 colon cancer cells.

Background and Objectives: Urtica dioica, a source of bioactive functional compounds, provides nutritional and gastrointestinal therapeutic benefits. This study attempted to investigate the prophylactic coloprotective action of an aqueous extract of Urtica dioica (AEUD) on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC). Materials and Methods: Phenolic compounds, total sugar, and mineral levels were determined in AEUD. Then, AEUD at different doses (50, 100, and 200 mg/kg, BW, p.o.) and mesalazine (MESA) as a standard treatment (100 mg/kg, BW, p.o.) were given orally for 21 days. Acute colitis was induced by administering drinking water with 5% (w/v) DSS for 7 days. Body weight variation, fecal occult blood, and stool consistency were determined daily. The severity of colitis was graded according to colon length, disease activity index (DAI), histological evaluations, and biochemical alterations. Rats orally administered DSS regularly developed clinical and macroscopic signs of colitis. Results: Due to its richness in phenolic and flavonoid compounds (247.65 ± 2.69 mg EAG/g MS and 34.08 ± 0.53 mg EQt/g MS, respectively), AEUD markedly ameliorated DAI, ulcer scores, colon length shortening, colonic histopathological changes, and hematological and biochemical modifications. Taken together, AEUD treatment notably (p < 0.01) suppressed DSS-induced UC by reducing oxidative stress via lowering MDA/H₂O₂ production and stimulating the effect of enzyme antioxidants as well as attenuating inflammation by decreasing CRP levels by 79.5% between the DSS and DSS + AEUD-50 groups compared to the MESA group (75.6%). Conclusions: AEUD was sufficient to exert a coloprotective effect that might be influenced by its bioactive compounds’ anti-inflammatory and antioxidant capabilities.

Background and Objectives:Urticadioica, a source of bioactive functional compounds, provides nutritional and gastrointestinal therapeutic benefits.The recent study attempted to investigate the prophylactic coloprotective action of aqueous extract of Urticadioica (AEUD) on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC). Materials and Methods: Phenolic compounds, total sugar, and mineral levels were determined in AEUD. Then, AEUD at different doses (50, 100, and 200 mg/kg, BW, p.o.) and Mesalazine (MESA), as a standard treatment (100 mg/kg, BW, p.o.), were given orally for 21 days. Acute colitis was induced by administering drinking water with 5% (w/v) DSS for 7 days. Body weight variation, fecal occult blood, and stool consistency were determined daily. The severity of colitis was graded by colon length, disease activity index (DAI), histological evaluations, and biochemical alterations. Rats orally administered with DSS regularly developed clinical and macroscopic signs of colitis.Results: Due to its richness in phenolic and flavonoid compounds (247.65±2.69 mg EAG/g MS and 34.08±0.53 mg EQt/g MS, respectively),AEUD markedly ameliorated DAI, ulcer scores, colon length shortening, colonic histopathological changes, and hematological and biochemical modifications. Taken together, AEUD-treatment suppressed notably (p< .01) DSS-induced UC by reducing oxidative stress via lowering MDA/H2O2 generation and stimulating effect of enzyme antioxidants as well as attenuating inflammation by decreasing CRP levels by 79.5% between DSS and DSS+AEUD-50 groups compared to MESA-group (75.6%).Conclusions: AEUD was sufficient to exert its coloprotectiveeffect which might be influenced by its bioactive compounds’ anti-inflammatory and antioxidant capabilities.

Background and Objectives: Obesity is currently a major health problem due to fatty acid accumulation and excess intake of energy, which leads to an increase in oxidative stress, particularly in the liver. The main goal of this study is to evaluate the protective effects of spirulina (SP) against cafeteria diet (CD)-induced obesity, oxidative stress, and lipotoxicity in rats. Materials and Methods: The rats were divided into four groups and received daily treatments for eight weeks as follows: control group fed a standard diet (SD 360 g/d); cafeteria diet group (CD 360 g/d); spirulina group (SP 500 mg/kg); and CD + SP group (500 mg/kg, b.w., p.o.) according to body weight (b.w.) per oral (p.o.). Results: Our results show that treatment with a CD increased the weights of the body, liver, and abdominal fat. Additionally, severe hepatic alteration, disturbances in the metabolic parameters of serum, and lipotoxicity associated with oxidative stress in response to the CD-induced obesity were observed. However, SP treatment significantly reduced the liver alteration of CD feed and lipid profile disorder associated with obesity. Conclusions: Our findings suggest that spirulina has a marked potential therapeutic effect against obesity and mitigates disturbances in liver function parameters, histological alterations, and oxidative stress status.