Jie Zhang

Root interactions are crucial in regulating soil microbial metabolism and plant nutrient allocation strategies, especially in mixed plantings. However, the effects of mixed planting and direct root contact on soil properties and plant nutrient allocation remain unclear. Thus, we established potted plants with Moso bamboo (Phyllostachys edulis) and Phoebe chekiangensis and created a physical barrier to the root system without blocking chemical communication using four treatments: mixed planting with root segregation (MT), mixed planting without root segregation (MS), pure Moso bamboo with root segregation (BT), and pure Moso bamboo without root segregation (BS). We investigated changes in soil and Moso bamboo nutrient content, soil enzyme activity, and microbial metabolic limitation. The results show that mixed planting and root segregation significantly affected soil and plant nutrient content and soil enzyme activities. Compared to the two pure Moso bamboo treatments, mixed planting increased microbial carbon limitation but decreased microbial nitrogen limitation. Physical segregation between roots increased microbial carbon use efficiency (CUE) compared to no segregation. Random forest analyses revealed that the best predictors of soil C and N limitations and CUE were microbial biomass and dissolved organic nitrogen (DON), respectively. Partial least squares path modeling indicated that mixed planting and root separation, directly and indirectly, affected soil microbial metabolic limitation through their effects on soil nutrients, microbial biomass, and enzyme activities. Carbon limitation significantly increased plant nutrient contents. Our study provides further insights into factors influencing nutrient limitation, CUE, and plant nutrient allocation strategies in mixed Moso bamboo plantations.

Intercropping is a promising strategy for sustainable medicinal plant cultivation, but its impact on plant–microbe interactions remains poorly understood. This study investigated the influence that intercropping giant lily (Cardiocrinum giganteum) with bamboo (BG), Chinese fir (FG), and mixed forests (MG) had on the giant lily metabolome and microbiome compared to a monoculture control (GG). Metabolomic analysis revealed that BG significantly increased the accumulation of terpenoids (e.g., yucalexin B22, 19.39-fold), alkaloids (e.g., anabasine, 2.97-fold), and steroids (e.g., O-acetyl-lariciresinol, 4.49-fold), while MG induced the production of stress-related metabolites (e.g., aflatoxin G2, 128.62-fold), and FG enhanced nitrogen metabolism (e.g., putrescine, 2.47-fold). Intercropping altered the rhizosphere and endophytic microbial communities, with BG enriching beneficial bacteria (e.g., Acidobacteria and Alphaproteobacteria) and FG promoting symbiotic fungi (e.g., Serendipita and Xylariales). Network analysis revealed strong correlations between specific microbial taxa (e.g., Bacillus and Ceratobasidiaceae) and key metabolites (e.g., norpandamarilactonine A, methylgingerol), indicating their potential roles in shaping the metabolic profiles of giant lily. These findings highlight the complex interplay between intercropping systems, microbial communities, and medicinal plant metabolism and provide a basis for developing targeted cultivation strategies to enhance the production of bioactive compounds in giant lily and other medicinal plants.

Intercropping affects soil microbial community structure significantly; however, the effects on understory medicinal plants in karst areas remain unclear. We investigated the effects of four intercropping systems (Moso bamboo, Chinese fir, bamboo-fir mixed forest, and forest gap) on the rhizosphere microbial communities of giant lily (Cardiocrinum giganteum), an economically important medicinal plant in China. We assessed the intercropping impact on rhizosphere microbial diversity, composition, and co-occurrence networks and identified key soil properties driving the changes. Bacterial and fungal diversity were assessed by 16S rRNA and ITS gene sequencing, respectively; soil physicochemical properties and enzyme activities were measured. Moso bamboo system had the highest fungal diversity, with relatively high bacterial diversity. It promoted a distinct microbial community structure with significant Actinobacteria and saprotrophic fungi enrichment. Soil organic carbon, total nitrogen, and available potassium were the most influential drivers of microbial community structure. Co-occurrence network analysis revealed that the microbial network in the Moso bamboo system was the most complex and highly interconnected, with a higher proportion of positive interactions and a greater number of keystone taxa. Thus, integrating Moso bamboo into intercropping systems can enhance soil fertility, microbial diversity, and ecological interactions in the giant lily rhizosphere in karst forests.

Bamboo invasion into broadleaf forests is a common phenomenon in karst areas; however, the effect of bamboo invasion on soil organic carbon (SOC) in karst areas and the mechanism of the effect are not clear. We selected the study site with broad-leaved forests (BF), mixed forests (MF), and pure bamboo (Indosasa shibataeoides McClure) forests (IF). Furthermore, we sampled the soil from 0 cm to 20 cm and 20 cm to 40 cm layers in the region and investigated the soil properties, organic carbon fractions, and microbial communities. At the same time, we sampled the litterfall layer of different stands and determined the biomass. The results showed that bamboo invasion increased the litterfall biomass per unit area of karst forest, increased the bulk weight of the 0–20 cm soil layer, and lowered the soil pH in the 0–20 cm and 20–40 cm soil layers, bamboo invasion consistently increased the content of soil AN and AK, whereas the content of AP was significantly reduced after bamboo invasion. Both active organic carbon groups (MBC, DOC, and EOC) and passive organic carbon groups (Fe/Al-SOC and Ca-SOC) increased significantly after bamboo invasion. The bamboo invasion increased the diversity of soil microorganisms and bacterial communities; the relative abundance of Actinobacteriota increased in MF and IF, while the relative abundance of Firmicutes decreased in IF. The structure of fungal communities was altered during the bamboo invasion, with an increase in the relative abundance of Mortierellomycota and a decrease in the relative abundance of Basidiomycota at the level of fungal phyla. Partial least squares path modeling analysis identified bamboo invasion enhanced SOC sequestration mainly by increasing litterfall biomass and altering the structure of the fungal community, and the effect of bacteria on SOC was not significant. Our study suggests that bamboo invasion of broadleaf forests is more favorable to soil organic carbon sequestration in karst areas.