Cheng Cheng Jiayi

To explore the protective effect and mechanism of isorhamnetin against oxidative injury caused by H2O2 to endothelial cell strain CRL1730 of human umbilical vein. H2O2 and endothelial cell strain CRL1730 were used, as a model of injured endothelial cell. Three levels of crude drugs areorhamnetin, 22.8 mu g/ml, 11.4 mu g/mL and 5.7 mu g/mL was added to the injured cell strain CRL1730 respectively. The cell injury was measured in terms of necrotic rate, quantities of von Wilebr and factor (vWf) and thrombomodulin (TM), lactate dehydrogenase (LDH) and intracellular free calcium ions through flow cytometry, ELISA, fluorescent spectrometer and laser scanning confocal microscopy respectively. Isorhamnetins @ 11.4 mu g/mL and 5.7 mu g/mL has significantly decreased EC necrotic rate, while the increased vWf concentration due to oxidant (200 mu mol/L of H2O2) was significantly decreased by 5.7 mu g/mL versus 11.4 and 22.8 mu g/mL isorhamnetin. Also, the increased in TM and LDH in injured cells was reversed to normal level with 5.7 to 11.4 mu g/mL isorhamnetin. These results suggest that isorhamnetin protect the integrity of cell membranes. Similarly, H2O2 treatment of cells elicited the release of intracellular calcium, however, 5.7 mu g/mL and 11.4 mu g/mL isorhamnetin dramatically inhibited transient release of intracellular calcium. This suggests that isorhamnetin, at lower concentration, could inhibit the IP3-sensitive calcium pool from releasing calcium, protecting VECs from injury by H2O2. Traditional Chinese herbs, hippophaerhamnoides have been recognized as safe and as a source of flavonoids,with strong cardiovascular protection. The results of this study revealed that isorhamnetin produce a strong effect on some targetspresent in ECs and thus, provide a basis for the future work targeted towards endothelial cells protection.

BACKGROUND:Nonalcoholic fatty liver disease (NAFLD) is a condition characterized by excessive fat accumulation in the form of triglycerides. The incidence of NAFLD and hyperlipidemia, with their associated risks of end-stage liver and cardiovascular ...

To study pharmacological mechanism of Laosun Kangfu tablet. Blood-activating and stasis-removing was tested by establishing cute cold pathogens induced blood stasis rat model and microcirculation disturbance state rat model by means of dripping adrenine on the mensentery. The resisting oxidation was tested by establishing the freely falling body damages models. The kidney-notifying was tested by Acetic acid hydrocortisone-caused kidney asthenia of mice. All of the experimental results showed that there were Significant differences between the treatment groups and the control groups, such as promoting blood circulation and removing blood stasis, resisting the oxidation, the kidney-notifying actions. Effects of Huoxue Xiaozhong and Xingqi Zhitong of Laosun Kangfu tablet is because the action of improving microcirculation, antioxidation and regulating endocrine.