Changan He

Drought stress is a major abiotic factor that adversely affects plant growth, development, and crop productivity. Lipoxygenases (LOXs) are key enzymes in the jasmonic acid (JA) biosynthetic pathway and play crucial roles in plant responses to environmental stresses. In this study, the ZmLOX2 gene was cloned from maize (Zea mays L.), and was found to encode a chloroplast-localized 13-lipoxygenase (13-LOX) protein. Expression analysis revealed that ZmLOX2 was predominantly expressed in leaves and was strongly induced under osmotic stress conditions. Compared with wild-type plants, transgenic Arabidopsis thaliana overexpressing ZmLOX2 exhibited significantly higher survival rates, enhanced growth performance, and improved root development under water deficit conditions. Physiological and biochemical analyses showed that the overexpression lines displayed higher activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), accompanied by significantly reduced malondialdehyde (MDA) content and reactive oxygen species (ROS) accumulation. Further investigation demonstrated that the expression levels of JA biosynthesis- and signaling-related genes, including ALLENE OXIDE SYNTHASE (AtAOS), 12-OXOPHYTODIENOATE REDUCTASE 3 (AtOPR3), MYC DOMAIN PROTEIN 2 (AtMYC2), and JASMONATE ZIM-DOMAIN 1 (AtJAZ1), were markedly upregulated in the transgenic lines. Protein–protein interaction analysis suggested that ZmLOX2 may interact with ALLENE OXIDE SYNTHASE 1 (AOS1), HYDROPEROXIDE LYASE 1 (HPL1), and ALPHA-DIOXYGENASE 1 (DOX1). Collectively, these results suggest that ZmLOX2 may contribute to plant adaptation to water limitation by regulating responses associated with JA, enhancing antioxidant defense, and maintaining ROS homeostasis.

Abiotic stress constrains plant growth and productivity worldwide. To survive adverse environmental conditions, plants deploy sophisticated adaptive strategies involving transcriptional reprogramming and metabolic remodeling. Over the past decade, advancements in high-throughput sequencing and mass spectrometry have propelled transcriptomics and metabolomics as pivotal post-genomic disciplines, offering unprecedented opportunities to dissect molecular mechanisms underlying stress responses. This review synthesizes current progress in applying these omics technologies to investigate plant adaptations to key abiotic stresses (thermal, saline, water deficit/excess, and heavy metal stresses). We systematically evaluate the technical strengths and limitations of transcriptomic and metabolomic platforms, highlight recent breakthroughs in stress-responsive gene identification and metabolic pathway elucidation, and discuss emerging challenges in integrative data analysis. By bridging genotype–phenotype relationships through multi-omics approaches, this study aims to deepen our mechanistic understanding of plant stress resilience and inform the design of stress-resilient crops for sustainable agriculture.

Low-temperature stress during the germination stage is an important abiotic stress that affects the growth and development of northern spring maize and seriously restricts maize yield and quality. Although some quantitative trait locis (QTLs) related to low-temperature tolerance in maize have been detected, only a few can be commonly detected, and the QTL intervals are large, indicating that low-temperature tolerance is a complex trait that requires more in-depth research. In this study, 296 excellent inbred lines from domestic and foreign origins (America and Europe) were used as the study materials, and a low-coverage resequencing method was employed for genome sequencing. Five phenotypic traits related to low-temperature tolerance were used to assess the genetic diversity of maize through a genome-wide association study (GWAS). A total of 14 SNPs significantly associated with low-temperature tolerance were detected (−log10(P) > 4), and an SNP consistently linked to low-temperature tolerance in the field and indoors during germination was utilized as a marker. This SNP, 14,070, was located on chromosome 5 at position 2,205,723, which explained 4.84–9.68% of the phenotypic variation. The aim of this study was to enrich the genetic theory of low-temperature tolerance in maize and provide support for the innovation of low-temperature tolerance resources and the breeding of new varieties.