Arnau Sans-Dublanc
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Research Interests
Arnau Sans-Dublanc has a total of 7 co-authors. Here are the top co-authors by number of shared publications:
- Alan Urban
KU Leuven
1 shared publication - Clément Brunner
Neuro-Electronics Research Flanders
1 shared publication - Emilie Macé
Brain-wide Networks Group, Department of Ophthalmology, Center for Biostructural Imaging of Neurodegeneration, University Medical Center Göttingen, Germany
1 shared publication - Gabriel Montaldo
Neuro-Electronics Research Flanders, Vlaams Instituut voor Biotechnologie, and Interuniversity Microelectronics Centre, Leuven, Belgium
1 shared publication - Karl Farrow
VIB-KU Leuven Center for Neuroscience, Leuven, Belgium; Department of Biology & Leuven Brain Institute, KU Leuven, Leuven, Belgium
1 shared publication - Micheline Grillet
Neuro-Electronics Research Flanders, Leuven, Belgium
1 shared publication
Latest Publications
- Open Access
A platform for brain-wide functional ultrasound imaging and analysis of circuit dynamics in behaving mice
SUMMARY: Imaging of large-scale circuit dynamics is crucial to gain a better understanding of brain function, but most techniques have a limited depth of field. Here we describe vfUSI, a platform for brain-wide volumetric functional ultrasound imaging of hemodynamic activity in awake head-fixed mice. We combined high-frequency 1024-channel 2D-array transducer with advanced multiplexing and high-performance computing for real-time 3D Power Doppler imaging at high spatiotemporal resolution (220×280×175-μm 3 voxel size, up to 6 Hz). In addition, we developed a standardized software pipeline for registration and segmentation based on the Allen Mouse Common Coordinate Framework, allowing for temporal analysis in 268 individual brain regions. We demonstrate the high sensitivity of vfUSI in multiple experimental situations where stimulus-evoked activity can be recorded using a minimal number of trials. We also mapped neural circuits in vivo across the whole brain during optogenetic activation of specific cell-types. Moreover, we revealed the sequential activation of sensory-motor regions during a grasping water droplet task. vfUSI will become a key neuroimaging technology because it combines ease of use, reliability, and affordability.
Open AccessA platform for brain-wide functional ultrasound imaging and analysis of circuit dynamics in behaving mice
SUMMARY: Imaging of large-scale circuit dynamics is crucial to gain a better understanding of brain function, but most techniques have a limited depth of field. Here we describe vfUSI, a platform for brain-wide volumetric functional ultrasound imaging of hemodynamic activity in awake head-fixed mice. We combined high-frequency 1024-channel 2D-array transducer with advanced multiplexing and high-performance computing for real-time 3D Power Doppler imaging at high spatiotemporal resolution (220×280×175-μm 3 voxel size, up to 6 Hz). In addition, we developed a standardized software pipeline for registration and segmentation based on the Allen Mouse Common Coordinate Framework, allowing for temporal analysis in 268 individual brain regions. We demonstrate the high sensitivity of vfUSI in multiple experimental situations where stimulus-evoked activity can be recorded using a minimal number of trials. We also mapped neural circuits in vivo across the whole brain during optogenetic activation of specific cell-types. Moreover, we revealed the sequential activation of sensory-motor regions during a grasping water droplet task. vfUSI will become a key neuroimaging technology because it combines ease of use, reliability, and affordability.