Johannes Frank
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Johannes Frank has a total of 16 co-authors. Here are the top co-authors by number of shared publications:
- Astrid Hafner
Institute of Applied Synthetic Chemistry, Faculty of Technical Chemistry, Technische Universitaet Wien, Getreidemarkt 9/163, 1060 Vienna, Austria
1 shared publication - Christian Resch
Science, Research, and Development Division, Austrian Federal Ministry of Defence, 1090 Vienna, Austria
1 shared publication - Eva Ingeborg Reihs
Karl Chiari Lab for Orthopaedic Biology, Department of Orthopedics and Trauma Surgery, Medical University of Vienna, 1090 Vienna, Austria
1 shared publication - Florian Selinger
TU Wien, Faculty of Technical Chemistry, Getreidemarkt 9, 1060 Vienna, Austria
1 shared publication - Gerald Bauer
Science, Research, and Development Division, Austrian Federal Ministry of Defence, 1090 Vienna, Austria
1 shared publication - Günter Povoden
CBRN-Defence-Centre, Austrian Armed Forces, 2100 Korneuburg, Austria
1 shared publication
Latest Publications
- Open Access
- 18 February 2024
- Published by MDPI AG in Biosensors
- Vol. 14 (2) , 107
Using Rapid Prototyping to Develop a Cell-Based Platform with Electrical Impedance Sensor Membranes for In Vitro RPMI2650 Nasal Nanotoxicology Monitoring
Due to advances in additive manufacturing and prototyping, affordable and rapid microfluidic sensor-integrated assays can be fabricated using additive manufacturing, xurography and electrode shadow masking to create versatile platform technologies aimed toward qualitative assessment of acute cytotoxic or cytolytic events using stand-alone biochip platforms in the context of environmental risk assessment. In the current study, we established a nasal mucosa biosensing platform using RPMI2650 mucosa cells inside a membrane-integrated impedance-sensing biochip using exclusively rapid prototyping technologies. In a final proof-of-concept, we applied this biosensing platform to create human cell models of nasal mucosa for monitoring the acute cytotoxic effect of zinc oxide reference nanoparticles. Our data generated with the biochip platform successfully monitored the acute toxicity and cytolytic activity of 6 mM zinc oxide nanoparticles, which was non-invasively monitored as a negative impedance slope on nasal epithelial models, demonstrating the feasibility of rapid prototyping technologies such as additive manufacturing and xurography for cell-based platform development.
- 18 February 2024
- Published by MDPI AG in Biosensors
- Vol. 14 (2) , 107
Open AccessUsing Rapid Prototyping to Develop a Cell-Based Platform with Electrical Impedance Sensor Membranes for In Vitro RPMI2650 Nasal Nanotoxicology Monitoring
Due to advances in additive manufacturing and prototyping, affordable and rapid microfluidic sensor-integrated assays can be fabricated using additive manufacturing, xurography and electrode shadow masking to create versatile platform technologies aimed toward qualitative assessment of acute cytotoxic or cytolytic events using stand-alone biochip platforms in the context of environmental risk assessment. In the current study, we established a nasal mucosa biosensing platform using RPMI2650 mucosa cells inside a membrane-integrated impedance-sensing biochip using exclusively rapid prototyping technologies. In a final proof-of-concept, we applied this biosensing platform to create human cell models of nasal mucosa for monitoring the acute cytotoxic effect of zinc oxide reference nanoparticles. Our data generated with the biochip platform successfully monitored the acute toxicity and cytolytic activity of 6 mM zinc oxide nanoparticles, which was non-invasively monitored as a negative impedance slope on nasal epithelial models, demonstrating the feasibility of rapid prototyping technologies such as additive manufacturing and xurography for cell-based platform development.