Belkis Tekgüler

Olive leaf tea (OLT), rich in phenolics, exhibits antioxidant, anti-inflammatory, and potential anticancer effects; however, the in vivo efficacy remains unclear. This study evaluated the chemopreventive and systemic effects of OLT in a murine Ehrlich Ascites Tumor (EAT) model, with a focus on the treatment timing. OLT was prepared by aqueous infusion and characterized for total phenolic content (TPC: 25.74 mg GAE/g), DPPH scavenging (197.88 µmol TE/g), FRAP activity (81.23 µmol Fe²⁺/g), and LC-MS/MS profile (oleuropein 77.6%). Mice received OLT orally before or after tumor inoculation. Prophylactic OLT reduced EAT cell counts (from 31.48 × 10⁷ to 21.15 × 10⁷), ascites volume (from 4.58 to 2.98 mL), elevated miR-155-5p (14.34-fold), normalized ALT/AST, and restored hepatic antioxidants without histopathological damage. Co-treatment with 5-FU preserved efficacy while reducing hepatotoxicity. In conclusion, OLT provides timing-dependent anticancer and systemic protective effects in the EAT model, supporting its potential as a cost-effective nutraceutical for cancer prevention and adjunctive therapy.

The wild Foxtail lily (Eremurus spectabilis) is a valuable vegetable consumed in different forms as food and employed as a remedy for preventive and curative purposes. In the present study, the physicochemical and antioxidant properties and the volatile compounds of the leaves and stem parts of wild Foxtail lily were determined. The moisture content, ash, pH, crude fibre, crude protein and crude fat were determined as 93.90% and 90.90%; 0.48% and 0.70%; 5.01 and 5.01; 0.62% and 1.06%; 1.4% and 2.27%; and 0.60% and 0.60% in stem and leaf, respectively. L*, a* and b* values were found to be 67.77 and 46.70; -8.99 and -12.85; and 22.54 and 17.48 in stem and leaf, respectively. The total phenolic content, FRAP and DPPH were found in stem and leaf as 897.75 and 1781.83 mg GAE kg-1; 5.26 and 12.29 mmol ISE g-1; and 40.81 and 89.55 mmol TE g-1, respectively. 11 volatile compounds were detected in Foxtail lily stem and leaf, including 3 aldehydes, 3 alcohols, 1 ester, 1 furan, 1 sulfur compound, 1 nitrogen compound and 1 aliphatic hydrocarbon. Acetaldehyde, dimethyl sulfide, methyl isocyanide and ethyl alcohol were the most important volatile compounds detected in the headspace of Foxtail lily. The leaf part presented the highest physico-chemical and antioxidant properties, while the headspace of stem provided the highest volatile compounds.

Pine resin has been used as a traditional health-promoting medicinal food in Turkey and some countries for centuries. In the present study, the antioxidant potential and the monoterpene profile of 13 pine resin samples purchased from herbalists in different provinces of Turkey were investigated. According to the analysis results, there were differences between the pine resin samples, especially in terms of color and antioxidant properties. The experimental results demonstrated that the pine resin had strong in-vitro antioxidant effects. Total phenolic, FRAP, and DPPH (EC50) values ranged between 23.19 and 379.44 mgGAE g-1, 68.85 and 758.80 µmol Fe2+ g-1, 54.36 and 1006.97 µg g-1, respectively. Total phenolic content values correlated well with the FRAP values. In contrast, it was found that there was a negative correlation between FRAP values and EC50 values (r=-0.719**) and between total phenolic substance and EC50 values (r=-0.688**). Also, the monoterpene compounds of the resin were determined by applying solid-phase microextraction (SPME) and gas chromatography-mass spectroscopy. Eight monoterpene compounds were identified in different pine resin samples, including α-pinene, camphene, β-pinene, 3-carene, β-myrcene, cymene, D-limonene, and p-cymene. Generally, the main monoterpenes were α-pinene and β-pinene. Çam reçinesi, yüzyıllardır Türkiye'de ve bazı ülkelerde geleneksel, sağlığı geliştirici tıbbi gıda olarak kullanılmaktadır. Bu çalışmada, Türkiye'nin farklı illerindeki aktarlardan satın alınan 13 adet çam reçinesi örneğinin antioksidan özellikleri ve monoterpen profili araştırılmıştır. Analiz sonuçlarına göre çam reçinesi örnekleri arasında özellikle renk ve antioksidan özellikler açısından farklılıklar tespit edilmiştir. Deneysel sonuçlar, çam reçinesinin güçlü in vitro antioksidan etkilere sahip olduğunu göstermiştir. Toplam fenolik, FRAP ve DPPH (EC50) değerleri sırasıyla 23.19 ve 379.44 mgGAE g-1, 68.85 ve 758.80 µmol Fe2+ g-1, 54.36 ve 1006.97 µg g-1 arasında değişmiştir. Toplam fenolik içerik değerleri, FRAP değerleri ile iyi bir korelasyon göstermiştir. Buna karşılık, FRAP değerleri ile EC50 değerleri (r=-0.719**) arasında ve toplam fenolik madde ile EC50 değerleri (r=-0.688**) arasında negatif korelasyon olduğu tespit edilmiştir. Ayrıca reçinenin monoterpen bileşikleri, katı faz mikroekstraksiyon (SPME) ve gaz kromatografisi-kütle spektroskopisi uygulanarak belirlenmiştir. Farklı çam reçinesi numunelerinde α-pinen, camphene, β-pinene, 3-carene, β-myrcene, cymene, D-limonen ve p-cymene olmak üzere sekiz monoterpen bileşiği tanımlanmıştır. Genel olarak, örneklerdeki başlıca monoterpenler α-pinen ve β-pinendir.

ISHS VII International Symposium on Edible Alliaceae Some physical and chemical characteristics of Taşk