Ishtiyaq Ahad

Experiment was carried out at farmers field near the Faculty of Agriculture, Wadura, Kashmir, India during 2021-22 to evaluate efficacy of various insecticides and botanicals against cereal leaf beetle Oulema melanopus (L.) (Coleoptera: Chrysomelidae) infesting Rabi Oats (Avena sativa L.) in North Kashmir. Among plant extracts and insecticides evaluated against O. melanopus, Abamectin proved to be most effective in causing huge mortality to grub population with decreasing number of grub were recorded as (24, 21.3, 8.3, 1.6 and 1 %) after 2,4,6,8 and 10 days of spray, respectively during 2021 followed by Thiamethoxam (32.6, 15.3, 8, 0.3%) after 2, 4, 6, 8 ad 10 days of spray. Same trend of results was observed in 2022 and the pooled data. Among tested botanicals viz. neem and Artimesia; Artimesia was effective in declining the grub population with (44, 42, 41, 39 and 37%) after 2, 4, 6,8 and 10 days after spray. Severe infestation has been observed at maturity of crop and keeping in view the use as fodder to cattle it is necessary to inculcate the use of locally available botanicals for better health of cattle during the dearth period of fodder in Kashmir valley. Overall, the marginal farmers might be saved from any untoward by utilization of the bioactive plant extracts in management of this notorious pest.

A population of entomopathogenic nematodes, belonging to the Feltiae-clade and labelled J13, was discovered in the agricultural soils of the hilly regions of the Union territory of Jammu and Kashmir, India. Based on morphological, morphometric, and molecular analyses, the nematodes were identified as Steinernema feltiae. The J13 nematode isolate was tested in a laboratory assay for its pathogenicity against six major pests of vegetable crops: Pieris brassicae, Plutella xylostella, Helicoverpa armigera, Agrotis iplison, Trichoplusia ni, and Exelastis atomosa. The morphology of the isolated nematode closely matched the original description, except for the adult females, which had prominent epiptygmata instead of the weakly developed, double-flapped epiptygmata described in the original report. Analysis of the internal transcribed spacer and large subunit rRNA data from the J13 nematodes showed 100% similarity to sequences of the type population, indicating that they are conspecific. The virulence assays revealed that the nematode caused 100% mortality in the tested insect pests within 48–72 hours, even at the lowest concentration of 50 infective juveniles per insect. The calculated median lethal concentration varied among the pests, with the lowest number of infective juveniles needed to achieve 50% larval killing being 117 for P. xylostella, 181.74 for P. brassicae, 226.35 for H. armigera, and 202.07 for T. ni at 24 hours post-inoculation. These findings suggest that S. feltiae isolated during the present investigation, may be a viable option for the biocontrol of these insect pests in Kashmir valley, India.