Cristina Di Fiore

Honey is recognized as a valuable tool for environmental biomonitoring, while its contaminant content is relevant to food safety, requiring analytical methods for the simultaneous determination of multiple contaminants. In this study, an ultrasound–vortex-assisted dispersive liquid–liquid microextraction (UVA-DLLME) procedure coupled with gas chromatography–ion trap mass spectrometry (GC–IT/MS) was developed for the simultaneous determination of pesticides, polycyclic aromatic hydrocarbons (PAHs), and polychlorinated biphenyls (PCBs) in honey. The extraction solvent composition was optimized using different n-heptane/toluene mixtures, and the selected conditions were validated in terms of linearity, recovery, sensitivity, and precision. The optimized method provided recoveries between 70.2 and 112%, coefficients of determination (R²) from 0.9389 to 0.9998, limits of detection ranging from 0.40 to 21 ng g⁻¹, and intraday and interday relative standard deviations below 15%. The environmental performance of the sample preparation procedure was evaluated using the AGREEprep metric, giving an overall score of 0.52. Application of the method to 21 artisanal honey samples showed a generally low occurrence of the investigated contaminants, with PAHs being the most frequently detected compounds, whereas pesticides and PCBs were mostly below the limits of detection or quantification. The proposed method represents a solvent-efficient analytical procedure suitable for the multiclass determination of organic contaminants in honey.

The present study investigates the potential of poplar (Populus spp.) biomass from phytoremediation plantations as a feedstock for downdraft fixed bed gasification. The biomass was characterized in terms of moisture, ash content, elemental composition (C, H, N, O), and calorific values (HHV and LHV), confirming its suitability for thermochemical conversion. Gasification tests yielded a volumetric syngas production of 1.79 Nm³ kg⁻¹ biomass with an average composition of H₂ 14.58 vol%, CO 16.68 vol%, and CH₄ 4.74 vol%, demonstrating energy content appropriate for both thermal and chemical applications. Alkali and alkaline earth metals (AAEM), particularly Ca (273 mg kg⁻¹) and Mg (731 mg kg⁻¹), naturally present enhanced tar reforming and promoted reactive gas formation, whereas heavy metals such as Cd (0.27 mg kg⁻¹), Pb (0.02 mg kg⁻¹), and Bi (0.01 mg kg⁻¹) were detected only in trace amounts, posing minimal environmental risk. The results indicate that poplar pruning residues from phytoremediation sites can be a renewable and sustainable energy resource, transforming a waste stream into a process input. In this perspective, the integration of soil remediation with syngas production constitutes a tangible model of circular economy, based on the efficient use of resources through the synergy between environmental remediation and the valorization and sustainable management of marginal biomass—i.e., pruning residues—generating environmental, energetic, and economic benefits along the entire value chain.

Indoor air quality (IAQ) has emerged as a critical area of research, reflecting growing concerns regarding occupant health, well-being, and comfort in enclosed environments. The increasing complexity of modern indoor spaces, coupled with rapid advancements in sensing technologies and data analysis methodologies, has intensified scientific interest in effective IAQ assessment and management. This review aims to examine current technologies and methodologies for monitoring key indoor air quality indicators. Furthermore, it offers practical recommendations for enhancing IAQ in diverse built environments and explores the integration of artificial intelligence (AI) into monitoring systems. The findings underscore the potential of AI-enhanced approaches to optimize indoor environmental conditions and support proactive air quality management strategies.

Indoor air quality (IAQ) is a crucial priority, especially since people spend most of their time indoors. Indoor air can be more polluted than outdoor air due to sources such as building materials, cleaning products, and heating systems. This condition can affect health and productivity, especially in schools and work environments. Students spend about a third of their day in classrooms, and studies have shown that poor IAQ can cause respiratory and allergic problems, especially among children, who are more vulnerable. Poor ventilation and excessive CO₂ concentration are indicators of suboptimal indoor air quality, which can lead to symptoms such as headaches, fatigue, and worsening asthma. In Italy, the lack of specific legislation on indoor air quality in schools is a problem, but improved ventilation, both natural and mechanical, and monitoring of CO₂ levels are recommended to prevent negative health consequences. This paper aims to describe a methodology to improve IAQ in schools. The paper discusses the results of a study conducted on CO₂ and PM₁₀ levels measured in real time in cold season (Nov–Mar) in different classrooms of primary and secondary schools present in a large Italian urban area in order to understand the IAQ state and identify possible improvement actions.

The exposure of humans to polycyclic aromatic hydrocarbons (PAHs) through fruits is a scarcely investigated topic. The atmospheric deposition of PAHs could contribute to such an issue. The present paper would like to propose an easy, fast, and routinary analytical method to extract and quantify PAHs in apples, pears, and grapes. Dispersive liquid–liquid microextraction allowed us to recover PAHs ranging between 68.0 ± 1.2 and 96.2 ± 0.8% from fruit. Gas chromatography equipped with flame ionization detector analysis showed satisfactory analytical parameters, with details like R² > 0.9912 in a concentration range of 0.5–500 µg mL⁻¹, with a variability ranging within 0.7–2.3%. Rural fruit samples were found to be more contaminated by PAHs compared to urban samples, likely due to the use of non-green fuels in rural areas considered in this study. Further in-depth research on this topic is strongly recommended due to the relevance of fruits in the Mediterranean diet.

The rapid spread and accumulation of microplastics (MPs) in environmental ecosystems result from extensive plastic usage. MPs have been found in both indoor and outdoor air. Outdoor MP levels vary widely across global cities, with reported ranges from 36 to 118 MPs m⁻² day⁻¹. However, differing measurement units complicate comparisons. Indoor MPs are particularly concerning due to the significant amount of time people spend indoors. For instance, MP concentrations in workplaces like reception areas and nail salons were found to be 309 ± 214 and 46 ± 55 MPs m⁻³, respectively. Technological limitations hinder the identification of MPs, with methods like µ-ATR-FTIR, µ-FTIR, and µ-Raman identifying MPs of different sizes. MPs smaller than 0.3 µm pose a health risk as they can be internalized in lung cells, while MPs larger than 10 µm are too large to enter alveolar macrophages. This review highlights the current understanding of airborne MPs, focusing on their sources, transport, and deposition mechanisms. It aims to provide a foundation for further studies to deeply assess the presence, abundance, and occurrence of MPs in aerosols, a subject that remains underexplored.

The exposure of humans to microplastics through food is a topic of great interest. Foods of marine origin, such as fish and salt, have been the most extensively studied in this regard. Conversely, foods considered less likely to be contaminated (such as dairy products) have been investigated to a lesser extent. This is the first study addressing the occurrence of microplastics in cheese. In this paper, we report the first analytical approach for cheese preparation, before a chemical analysis of microplastics in cheese was performed. Therefore, the most suitable digestion methods were investigated. Alkaline digestion (i.e., KOH 5 M, 50 °C, 48 h) achieved a digestion efficiency of 97.5 ± 0.8%. To assess the feasibility of the preparative method proposed, a recovery rate of spiked polystyrene microbeads (~10 µm) of 98.5 ± 0.4% was determined. Further, the effects of the digestion agent on the microbeads were also investigated. To confirm whether the preparative method allows for the confirmation of the plastic-nature of microparticles, a qualification of spiked microplastics (polystyrene, 150 µm, and polyethylene terephthalate, 300 µm) was performed using pyrolysis–gas chromatography–mass spectrometry.

Phthalate esters (PAEs) are a group of chemicals used to improve the flexibility and durability of plastics. The chemical properties and the resistance to high temperatures promote their degradation and release into the environment. Food and beverages can be contaminated by PAEs through the migration from packaging material because they are not covalently bound to plastic and also via different kinds of environmental sources or during processing. For instance, alcoholic drinks in plastic containers are a particular risk, since the ethanol contained provides a good solubility for PAEs. According to its role as an endocrine disruptor compound and its adverse effects on the liver, kidney, and reproductive and respiratory systems, the International Agency on Research Cancer (IARC) classified di-(2-ethylhexyl) phthalate (DEHP) as a possible human carcinogen. For this reason, to control human exposure to PAEs, many countries prohibited their use in food as non-food substances. For example, in Europe, the Commission Regulation (EU) 2018/2005 restricts the use of DEHP, dibutyl phthalate (DBP), benzyl butyl phthalate (BBP), and diisobutyl phthalate (DiBP) to a concentration equal to or below 0.1 by weight in plasticizers in articles used by consumers or in indoor areas. There are reports from the US Food and Drug Administration (FDA) that some beverages (and food as well), particularly fruit juices, contain high levels of phthalates. In some cases, the deliberate adulteration of soft drinks with phthalate esters has been reported. This paper would like to show the difficulties of performing PAE analysis in beverage matrices, in particular alcoholic beverages, as well as the main solutions provided for quality control in the industrial branches.

Per- and polyfluoroalkyl substances (PFASs) are carbon–fluorine compounds with widespread industrial and domestic use, posing potential toxicological risks to humans and ecosystems. Several analytical methods have been developed to assess the occurrence of PFASs in the environment, but a standardized method, applicable to all matrices, is still lacking. This paper reviews the extraction and chromatographic detection methods for PFAS assessment in environmental samples, considering parameters such as the LOD, LOQ, and recoveries. Solid phase extraction (SPE) is commonly used, showing high recovery rates for water, soil, and sediment samples using HBL and WAX polymeric sorbents (85–100% and 93–111.5%, respectively). LC-MS has demonstrated low LODs and LOQs in seawater (0.01–0.08 ng L⁻¹; 0.03–0.24 ng L⁻¹), marine sediment (0.002–0.018 ng g⁻¹; 0.004–0.054 ng g⁻¹), and dust (0.08–0.68 pg g⁻¹; 0.26–2.25 pg g⁻¹), indicating its sensitivity when detecting trace PFAS levels. Evaluating PFASs is crucial for the development of future removal strategies and risk assessments. Potential solutions including the use of PFAS substitutes and innovative adsorption techniques for their adsorption could present promise in reducing their environmental presence.