Domingo Martínez-Soto

This mini-review synthesizes four decades of Mexican fusariosis literature, organizing cases into four clinical categories: ocular, superficial/cutaneous, invasive/disseminated, and central nervous system (CNS) disease. It identified significant gaps in national surveillance, molecular diagnostics, antifungal susceptibility testing, access to effective therapies, specialized mycology infrastructure, and highlights the urgent need for a coordinated clinical mycology network in Mexico. Fusariosis, caused by Fusarium species, presents a broad clinical spectrum, from keratitis and onychomycosis in immunocompetent individuals to disseminated disease in those with hematologic conditions. In Mexico, most reported cases stem from clinical case reviews rather than systematic national surveillance. Exceptions include a 10-year retrospective study published in 2023, and a Fusarium solani meningitis outbreak in Durango and Matamoros during 2022–2023, which together represent the largest global clusters of healthcare-associated fungal meningitis including an antifungal-resistant isolate in Matamoros that required investigational treatments such as Fosmanogepix. Keratitis by Fusarium spp. is the most documented manifestation, with 380 cases reported, most of them in clinical centers for diagnosis in Ciudad de Mexico, where F. solani predominated and ocular trauma was the primary risk factor. Additionally, 49 cases of invasive fusariosis have been reported across six states, mainly linked to burn injuries and hematological malignancies. Collectively, this review provides a national framework for understanding fusariosis and informs future research and public health strategies to address this emerging threat.

Organic vanilla production in Mexico holds significant promise but faces substantial challenges that impact its sustainability and market competitiveness. As the native region of Vanilla planifolia, Mexico is endowed with rich biodiversity and a deep cultural heritage surrounding vanilla cultivation. Organic production systems in the country predominantly rely on traditional agroforestry practices, manual pollination, and artisanal curing methods, all of which enhance the quality and distinctiveness of Mexican vanilla. However, production is hindered by critical factors, including low genetic diversity and susceptibility to phytopathogenic diseases, particularly stem and root rot caused by Fusarium oxysporum. In recent years, the application of in vitro micropropagation techniques has shown great potential for obtaining pathogen-free plants and conserving germplasm, offering a sustainable alternative to strengthen organic systems and reduce pressure on wild populations. The labor-intensive processes, yield variability, and the complexity of adhering to organic certification standards are additional challenges to overcome. Shifts in consumer preferences toward natural and sustainably produced goods have increased demand for organic vanilla, offering Mexican producers an opportunity to gain a more prominent position in the global market. Advancing research into disease management, fostering genetic conservation, and integrating scientific advances with traditional know-how are vital strategies for overcoming current limitations. In this context, organic vanilla production represents not only an economic opportunity but also a means to conserve biodiversity, support rural communities, and maintain the legacy of one of Mexico’s most emblematic agricultural products. This review was conducted using a qualitative, narrative analysis of recent scientific literature, technical reports, and case studies related to organic vanilla production in Mexico.

Background/Objectives: Botrytis cinerea is a necrotrophic fungal plant pathogen responsible for the gray mold disease, affecting several crops of economic importance worldwide. The primary line of control for the disease in the field and post-harvest fruits includes the application of fungicides. However, the emergence of fungal populations resistant to one or more fungicides has increased their application and diminished their effectiveness. Looking at new control strategies, metallic nanoparticles have appeared as a promising alternative for disease treatment. Green-synthesized copper oxide nanoparticles (CuONPs) are considered a feasible alternative, aiming to reduce the generation of environmentally toxic waste through chemical methods. Methods: In this work, CuONPs biosynthesized using the supernatant of Trichoderma asperellum and Trichoderma ghanense were evaluated to determine their antifungal activity against B. cinerea. Results: Four different formulations of CuONPs were obtained: Ta1, Ta2, Tg1, and Tg2. All formulations displayed antifungal properties, with Tg2 being the most effective and having a high potential in controlling the phytopathogen. CuONPs in the Tg2 formulation were quasi-spherical, ranging in size from 1 to 2.7 nm. Conclusions: Furthermore, Tg2 demonstrated greater efficacy than the copper-based commercial fungicide NORDOX^® 75W, which showed no inhibitory effect on B. cinerea mycelial growth. In summary, the CuONPs reported in this work offer a sustainable and effective alternative for managing the gray mold disease.

Grape production is one of the most agronomically important activities worldwide. However, it is threatened by diseases caused by phytopathogenic microorganisms, which cause severe economic losses. The primary strategy to control phytopathogenic fungi is the application of fungicides; however, they affect the environment and induce resistance in fungi. Nanomaterials, especially those green-synthesized, emerge as an eco-friendly and sustainable alternative to control fungal pathogens. The objective of this work is to evaluate the sensitivity of fungal phytopathogens to biosynthesized copper-oxide nanoparticles (CuONPs). Nanoparticles were evaluated as preventive and corrective treatments in grapevine green tissues and fruits under field conditions, using in vitro and in vivo experimental approaches. Interestingly, corrective treatment was highly effective and showed little accumulation of Cu on the fruits, even less than a commercial copper-based fungicide. Moreover, we report that Aspergillus niger causes lesions in photosynthetic tissues and severe disease symptoms in grapes. We also describe for the first time the presence of Alternaria alternata causing lesions, mainly on the stems and young leaves of grapevine plants in Mexico. These pathogens were inhibited by the biosynthesized CuONPs. All these findings show the effectiveness of using CuONPs to control phytopathogenic fungi, even under field conditions, shedding light on their potential use in agriculture with a less environmental impact than the commercial fungicides and agrochemicals currently used.

This paper discusses information collected from original articles published between 1992 and 2022 regarding heavy metals (HMs) contamination in various environments across Mexico. The primary aim of this work was to identify the Mexican states where concentrations of HMs have been reported to exceed the maximum permissible limits for several types of soil, water, and sediment according to Mexican standards NOM-147-SEMARNAT/SSA1-2004, NOM-127-SSA1-2021, as well as international standards. The data collected indicates that 25 states in Mexico have reported at least one metal exceeding the maximum permissible limits in soil. Among these, Zacatecas, Nuevo Leon and Chihuahua had the highest number of HMs exceeding the standards. For sediment contamination, 26 states exceeded the permissible limits, with San Luis Potosí and Guerrero showing the highest number of HMs above the standards. Additionally, 26 states have reports of HMs exceeding the permissible limits in water, with Guanajuato and Guerrero having the highest number of HMs. Interestingly, the most frequent metals reported as soil contaminants are Cu, Fe, Pb and Zn; in sediment, they are Cd, Cr, Cu, Fe, Pb and Zn; and in water, they are Cd, Cr, Cu, Fe, Mn, Pb and Zn. The compiled information indicates that the primary anthropogenic sources of HMs release in Mexico include industrial activities, urban wastewater, mining, and agricultural practices. Furthermore, the data analyzed highlights several serious health risks associated with exposure to HMs, including cancer, central nervous system damage, DNA damage, and issues related to kidneys and lungs. This paper provides a comprehensive overview of HMs contamination in Mexico as well as the health challenges that arise from this contamination. .

Study on plant differentiation between beneficial and pathogenic microorganisms How do plants differentiate friend from foe?. Answering this question is critical to understanding how plants recognize and allow their colonization by beneficial microorganisms, which improve their fitness, adaptation, and survival in hostile environments while deploying a multilayer immune system that thwarts pathogen colonization and avoids diseases (Figure 1). The multidisciplinary works published in this Research Topic contribute to answering this burning question in the plant interaction with fungi, bacteria, viruses, and microbial communities.