Pritpal Kaur
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Research Interests
Pritpal Kaur has a total of 6 co-authors. Here are the top co-authors by number of shared publications:
- Divya Srikumaran
Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland
1 shared publication - Jennifer Lu
Center for Computational Biology, Johns Hopkins University, Baltimore, MD 21218, USA
1 shared publication - Loren Moon
The Wilmer Eye Institute, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA
1 shared publication - Patricia J. Simner
Johns Hopkins University School of Medicine
1 shared publication - Steven L. Salzberg
Center for Computational Biology, Johns Hopkins University , Baltimore, Maryland, USA
1 shared publication - Uri S. Soiberman
Wilmer Eye Institute, Johns Hopkins Medical Institutions, Baltimore, MD 21287, USA
1 shared publication
Latest Publications
- Open Access
- 11 June 2024
- Published by MDPI AG in Journal of Clinical Medicine
- Vol. 13 (12) , 3399
No Evidence of Chronic Infection in a Metagenomic Sequencing Study of the Keratoconus Corneal Epithelium
Objectives: This study aims to assess the presence of pathogenic microorganisms in the corneal epithelial layer of keratoconus patients. Methods: DNA was extracted from corneal epithelial samples procured from ten individual keratoconus eyes and three healthy controls. Metagenomic next-generation sequencing (mNGS) was performed to detect ocular microbiota using an agnostic approach. Results: Metagenomic sequencing revealed a low microbial read count in corneal epithelial samples derived from both keratoconus eyes (average: 530) and controls (average: 622) without a statistically significant difference (p = 0.29). Proteobacteria were the predominant phylum in both keratoconus and control samples (relative abundance: 72% versus 79%, respectively). Conclusions: The overall low microbial read count and the lack of difference in the relative abundance of different microbial species between keratoconus and control samples do not support the hypothesis that a chronic corneal infection is implicated in the pathogenesis of keratoconus. These findings do not rule out the possibility that an acute infection may be involved in the disease process as an initiating event.
- 11 June 2024
- Published by MDPI AG in Journal of Clinical Medicine
- Vol. 13 (12) , 3399
Open AccessNo Evidence of Chronic Infection in a Metagenomic Sequencing Study of the Keratoconus Corneal Epithelium
Objectives: This study aims to assess the presence of pathogenic microorganisms in the corneal epithelial layer of keratoconus patients. Methods: DNA was extracted from corneal epithelial samples procured from ten individual keratoconus eyes and three healthy controls. Metagenomic next-generation sequencing (mNGS) was performed to detect ocular microbiota using an agnostic approach. Results: Metagenomic sequencing revealed a low microbial read count in corneal epithelial samples derived from both keratoconus eyes (average: 530) and controls (average: 622) without a statistically significant difference (p = 0.29). Proteobacteria were the predominant phylum in both keratoconus and control samples (relative abundance: 72% versus 79%, respectively). Conclusions: The overall low microbial read count and the lack of difference in the relative abundance of different microbial species between keratoconus and control samples do not support the hypothesis that a chronic corneal infection is implicated in the pathogenesis of keratoconus. These findings do not rule out the possibility that an acute infection may be involved in the disease process as an initiating event.