Jingjing Wang
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Jingjing Wang has a total of 10 co-authors. Here are the top co-authors by number of shared publications:
- Changbin Liu
Institute of Animal Husbandry and Veterinary Science, Xinjiang Academy of Agricultural Reclamation Sciences, Shihezi 832000, China; National Key Laboratory of Genetic Improvement and Healthy Sheep Breeding, Shihezi 832000, China
1 shared publication - Cheng Chen
College of Animal Science and Technology, Shihezi University, Shihezi 832000, China
1 shared publication - Cunxi Nie
College of Animal Science and Technology, Shihezi University, Shihezi, China
1 shared publication - Guangyu Feng
College of Animal Science and Technology, Shihezi University, China
1 shared publication - Limin Wang
State Key Laboratory of Sheep Genetic Improvement and Healthy Breeding, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China
1 shared publication - Minghui Liu
College of Animal Science and Technology, Shihezi University, China
1 shared publication
Latest Publications
- Open Access
- 19 July 2024
- Published by MDPI AG in Preprints.org
Electroporation Delivery of Cas9 sgRNA ribonucleoprotein-Mediated Genome Editing in Sheep IVF Zygotes
The utilization of electroporation for delivering the CRISPR/Cas9 system components has enabled efficient gene editing in mammalian zygotes, facilitating the development of genome-edited animals. In our study，This research focused on targeting the ACTG1 and MSTN genes in sheep, revealing a threshold phenomenon in electroporation with a voltage tolerance in sheep in vitro fertilization (IVF) zygotes. Various poring voltages near 40V and pulse durations were examined for electroporating sheep zygotes. The study concluded that stronger electric fields required shorter pulse durations to achieve the optimal conditions for high gene mutation rates and reasonable blastocyst development. The investigation also assessed the quality of Cas9/sgRNA ribonucleoprotein complexes (Cas9 RNPs) and their influence on genome editing efficiency in sheep early embryos. It was highlighted that pre-complexation of Cas9 proteins with single-guide RNA (sgRNA) before electroporation was essential for achieving a high mutation rate. The use of suitable electroporation parameters for sheep IVF zygotes led to significantly high mutation rates and heterozygote ratios. By delivering Cas9 RNPs and single-stranded oligodeoxynucleotides (ssODNs) to zygotes through electroporation, targeting the MSTN (Myostatin) gene, a knock-in efficiency of 26% was achieved. The successful generation of MSTN-modified lambs was demonstrated by delivering Cas9 RNPs into IVF zygotes via electroporation.
- 19 July 2024
- Published by MDPI AG in Preprints.org
Open AccessElectroporation Delivery of Cas9 sgRNA ribonucleoprotein-Mediated Genome Editing in Sheep IVF Zygotes
The utilization of electroporation for delivering the CRISPR/Cas9 system components has enabled efficient gene editing in mammalian zygotes, facilitating the development of genome-edited animals. In our study，This research focused on targeting the ACTG1 and MSTN genes in sheep, revealing a threshold phenomenon in electroporation with a voltage tolerance in sheep in vitro fertilization (IVF) zygotes. Various poring voltages near 40V and pulse durations were examined for electroporating sheep zygotes. The study concluded that stronger electric fields required shorter pulse durations to achieve the optimal conditions for high gene mutation rates and reasonable blastocyst development. The investigation also assessed the quality of Cas9/sgRNA ribonucleoprotein complexes (Cas9 RNPs) and their influence on genome editing efficiency in sheep early embryos. It was highlighted that pre-complexation of Cas9 proteins with single-guide RNA (sgRNA) before electroporation was essential for achieving a high mutation rate. The use of suitable electroporation parameters for sheep IVF zygotes led to significantly high mutation rates and heterozygote ratios. By delivering Cas9 RNPs and single-stranded oligodeoxynucleotides (ssODNs) to zygotes through electroporation, targeting the MSTN (Myostatin) gene, a knock-in efficiency of 26% was achieved. The successful generation of MSTN-modified lambs was demonstrated by delivering Cas9 RNPs into IVF zygotes via electroporation.