Fernanda dos Anjos Souza

Papillomaviruses (PVs) are double-stranded DNA viruses known to induce a variety of epithelial lesions in dogs, ranging from benign hyperplasia to malignancies. In regions of rich biodiversity such as the Western Amazon, data on the circulation and genetic composition of canine papillomaviruses (CPVs) remain scarce. This study investigated CPV types present in oral and cutaneous papillomatous lesions in domiciled dogs from Acre and Rondônia States, Brazil. Sixty-one dogs with macroscopically consistent lesions were clinically evaluated, and tissue samples were collected for histopathological examination and PCR targeting the L1 gene. Among these, 37% were histologically diagnosed as squamous papillomas or fibropapillomas, and 49.2% (30/61) tested positive for papillomavirus DNA. Sequencing of the L1 gene revealed that most positive samples belonged to CPV1 (Lambdapapillomavirus 2), while one case was identified as CPV8 (Chipapillomavirus 3). Complete genomes of three CPV1 strains were obtained via high-throughput sequencing and showed high identity with CPV1 strains from other Brazilian regions. Phylogenetic analysis confirmed close genetic relationships among isolates across distinct geographic areas. These findings demonstrate the circulation of genetically conserved CPVs in the Amazon and reinforce the value of molecular and histopathological approaches for the accurate diagnosis and surveillance of viral diseases in domestic dogs, especially in ecologically complex regions.

Papillomaviruses (PVs) are double-stranded DNA viruses known to induce a variety of epithelial lesions in dogs, ranging from benign hyperplasia to malignancies. In regions of rich biodiversity such as the Western Amazon, data on the circulation and genetic composition of canine papillomaviruses (CPVs) remain scarce. This study investigated CPV types present in oral and cutaneous papillomatous lesions in domiciled dogs from Acre and Rondônia States, Brazil. Sixty-one dogs with macroscopically consistent lesions were clinically evaluated, and tissue samples were collected for histopathological examination and PCR targeting the L1 gene. Among these, 37% were histologically diagnosed as squamous papillomas or fibropapillomas, and 49.2% (30/61) tested positive for papillomavirus DNA. Sequencing of the L1 gene revealed that most positive samples belonged to CPV1 (Lambdapapillomavirus 2), while one case was identified as CPV8 (Chipapillomavirus 3). Complete genomes of three CPV1 strains were obtained via high-throughput sequencing and showed high identity with CPV1 strains from other Brazilian regions. Phylogenetic analysis confirmed close genetic relationships among isolates across distinct geographic areas. These findings demonstrate the circulation of genetically conserved CPVs in the Amazon and reinforce the value of molecular and histopathological approaches for accurate diagnosis and surveillance of viral diseases in domestic dogs, especially in ecologically complex regions.

Bovine papillomaviruses (BPVs) have been widely characterized from cutaneous warts in cattle worldwide. However, there are still limited studies addressing the geographic distribution of viral types and their potential associations with the histopathological characteristics of lesions, particularly in the vast and ecologically diverse Amazon region. This study aimed to histologically and phylogenetically characterize cutaneous papillomatous lesions in cattle from the Vale do Guaporé, located in the Brazilian Western Amazon. A total of 54 wart samples were collected from 44 cattle clinically diagnosed with cutaneous papillomatosis. Histopathological analysis classified 58.33% of cases as fibropapillomas and 39.58% as squamous papillomas. Molecular analysis, based on L1 gene amplification and sequencing, identified the presence of previously reported BPV types (BPV2, 4, 5, 12, 13, and 15), along with a novel BPV14 subtype and three putative new types (PNT). Statistical analysis revealed that BPV2 was significantly associated with fibropapillomas (p = 0.023), whereas BPV13 was linked to cauliflower-like morphological lesions (p = 0.008). These findings enhance the understanding of BPV diversity circulating in cattle from the Amazon region and provide valuable insights into the clinicopathological aspects of bovine cutaneous papillomatosis, which may aid in future epidemiological surveillance and disease control strategies.

Bovine papillomaviruses (BPVs) have been widely characterized from cutaneous warts in cattle worldwide. However, there are still limited studies addressing the geographic distribution of viral types and their potential associations with the histopathological characteristics of lesions, particularly in the vast and ecologically diverse Amazon region. This study aimed to histologically and phylogenetically characterize cutaneous papillomatous lesions in cattle from the Vale do Guaporé, located in the Brazilian Western Amazon. A total of 54 wart samples were collected from 44 cattle clinically diagnosed with cutaneous papillomatosis. Histopathological analysis classified 58.33% of cases as fibropapillomas and 39.58% as squamous papillomas. Molecular analysis, based on L1 gene amplification and sequencing, identified the presence of previously reported BPV types (BPV2, 4, 5, 12, 13, and 15), along with a novel BPV14 subtype and three putative new types (PNT). Statistical analysis revealed that BPV2 was significantly associated with fibropapillomas (p = 0.023), whereas BPV13 was linked to cauliflower-like morphological lesions (p = 0.008). These findings enhance the understanding of BPV diversity circulating in cattle from the Amazon region and provide valuable insights into the clinicopathological aspects of bovine cutaneous papillomatosis, which may aid in future epidemiological surveillance and disease control strategies.

The Bos Taurus Papillomavirus, commonly known as bovine papillomavirus (BPV), can cause lesions in the mucosa of the gastrointestinal tract (GIT) in cattle and induce the formation of papillomas in organs such as the pharynx, esophagus, rumen and reticulum. GIT papillomas can lead to feeding and breathing distress. Moreover, the sample collection is challenging, which reduces the BPV diagnosis in these organs. BPV can cause exophytic nodular, cauliflower-like, flat, filiform or atypical-shape papillomas at the epidermis. Histologically, the papillomas demonstrate orthokeratotic/parakeratotic hyperkeratosis and koilocytosis and, currently, BPV comprises 45 described types. The aim of this study was to carry out the genetic characterization of BPV present in rumen neoplastic lesions of cattle raised extensively in the Western Amazon region, Brazil. A total of 100 papillomatous ruminal samples were collected from animals slaughtered in Ji-Paraná and Urupá municipalities from the Rondônia state, Brazil. The samples were submitted to PCR using the primer pair FAP59/FAP64 and sequenced by the Sanger method. Histopathological analysis was performed on 24 samples, which had enough material for this purpose. As a result, samples were histologically classified as fibropapilloma and squamous papilloma. Among the samples analyzed, it was possible to identify the BPVs 2, 13 (Delta PVs) and 44, with one sample classified as a putative new subtype of BPV44. The present study could identify BPV13 and 44 types in cattle rumen tissues from the Brazilian Amazon region for the first time.

A torção esplênica em cães é uma condição rara, caracterizada pela rotação do baço em torno dos ligamentos gastroesplênico e frenoesplênico. A condição é frequentemente associada à dilatação e vólvulo gástrico, mas também pode ocorrer de forma isolada tendo maior incidência em cães de grande porte. O diagnóstico baseia-se no histórico do animal, exames físicos, laboratoriais e complementares, como achados ultrassonográficos e o diagnóstico definitivo está condicionado à laparotomia exploratória. O presente estudo relata o caso de torção esplênica isolada em um cão da raça Fila Brasileiro, de nove anos de idade, adulto, macho, com 47,7 kg, apresentando vômito e anorexia. No exame ultrassonográfico o baço se apresentava acentuadamente aumentado, ocupando grande parte da cavidade abdominal e impedindo a visualização de outros órgãos. Estava difusamente hipoecóico com ecotextura rendilhada e característica de infarto/necrose. Na avaliação Doppler colorida se observou ausência de fluxo sanguíneo no parênquima esplênico, sinais sugestivos para torção e infarto. Tendo em vista a resolução do quadro, utilizou-se da técnica de celiotomia mediana pré-umbilical para a exploração da cavidade abdominal e, durante o procedimento, foi confirmado o quadro de torção do baço em torno de seu pedículo vascular, caracterizando a torção esplênica primária. O órgão estava gravemente aumentado e com coloração escura. O órgão retirado foi submetido ao exame anatomopatológico, que identificou intensa necrose esplênica associada a congestão e hemossiderose moderada. Após 11 dias, o animal retornou à clínica para reavaliação, onde apresentou-se estável. Acredita-se que o grande porte da raça Filla Brasileiro e o quadro de hemoparasitose tenham atuado como fatores predisponentes para o desenvolvimento da TEP pelo animal do presente relato.

As tripanossomíases são um conjunto de doenças causadas por protozoários do gênero Trypanosoma. Entre elas, temos a surra ou mal-das-cadeiras, cujo agente etiológico é o Trypanosoma evansi, importante patógeno transmitido por moscas hematófagas, como tabanídeos e moscas do gênero Stomoxys. Além dos equinos, o T. evansi pode infectar e causar patologia importante em cães. Entretanto, há poucos relatos sobre epidemiologia, diagnóstico, patologia e terapêutica em cães no Brasil, a escassez de dados é ainda maior quando se trata de casos nos estados da Amazônia legal, como Rondônia. Este trabalho tem como objetivo relatar o primeiro caso de infecção natural por T. evansi em cão no município de Ariquemes, Rondônia, Brasil. Para tal, foram realizados exame físico do paciente e laboratoriais. Foi feito hemograma, análise parasitológica de esfregaços sanguíneos e PCR (polymerase chain reaction). A análise por microscopia de luz mostrou a presença de flagelados do gênero Trypanosoma com ausência do cinetoplasto, características das formas tripomastigotas sanguíneas de T. evansi encontradas na América do Sul. O diagnóstico para tripanossoma foi confirmado por PCR convencional. No exame físico, observou-se mucosas hiperêmicas, hipetermia, desidratação, taquicardia, dor a palpação abdominal, pelagem opaca e ptose bilateral. O hemograma evidenciou moderada anemia regenerativa, microcítica, normocrômica, além de leucopenia, linfopenia e trombocitopenia. O tratamento do paciente foi realizado, no entanto, devido à falha de execução pelo proprietário, o paciente veio à óbito. Este trabalho vem mostrar a necessidade de uma pesquisa epidemiológica criteriosa sobre T. evansi no estado de Rondônia, realizando a busca por possíveis vetores e hospedeiros vertebrados domésticos e selvagens com auxílio de ferramentas moleculares, como PCR e sequenciamento de DNA, sendo estas, indispensáveis para uma caracteriazação precisa da espécie do parasito e das cepas circulantes na região.

A Piometra de Coto ou de Colo de útero é uma patologia incomum em fêmeas caninas e em gatas castradas, que tem como provável motivo a síndrome do ovário remanescente, podendo acontecer em animais após a ováriosalpingohisterectomia OSH) incompleta. O exame de ultrassonografia é o método para o diagnóstico. Entretanto, esse tipo de piometra normalmente ocorre quando os ovários não são retirados, podendo ser causado mais provavelmente por infecções bacterianas. Nesse contexto, o presente trabalho teve como objetivo relatar o caso de uma cadela sem raça definida (SRD), castrada, portadora de piometra de coto uterino com rompimento do ureter esquerdo, foi utilizado um procedimento inédito no qual a equipe usou uma sutura "Bolsa de Tabaco", contínua, em bolsa e invaginante para cobrir o ureter na parede muscular, fixando o mesmo na região da vesícula urinária e fixando o omento maior na região da vesícula urinária para cobri-la. A equipe cirúrgica denominou esta manobra de "Técnica de Rafipexia de Ureter em Vesícula Urinária Unilateral Silvagoes” por ser a composição de seus sobrenomes.