Xinyi Zeng

Objective: Kidney renal clear cell carcinoma (KIRC, ccRCC) is the most common type of renal cancer with high recurrence and mortality. It has long been recognized that Antizyme inhibitor 1 (AZIN1) serves as a pro‐oncogenic molecule in multiple cancers. However, the clinicopathological features of AZIN1 in KIRC remain unexplored.Materials and Methods: The Cancer Genome Atlas (TCGA, TIMER, and GEPIA) were employed for pan‐cancer expression and survival analysis of AZIN1, indicating the unique anti‐tumor role of AZIN1 in KIRC. The expression and clinical characteristics of AZIN1 in KIRC were further proven via Human Protein Atlas and TCGA. single‐sample GSEA was employed to investigate the immune infiltration of AZIN1. Then the downstream pathways were illustrated via the LinkedOmics, Metascape, and Cytoscape databases. The possible upper regulating noncoding RNAs (ncRNAs) were analyzed from five programs‐TargetScan, StarBase, miRanda, PITA, and miRmap.Results: AZIN1 is downregulated in KIRC patients. Lower levels of AZIN1 were linked with unfavorable outcomes in KIRC patients. The AZIN1 expression was positively related to immune cell infiltration in KIRC. We also elucidated a possible upstream regulatory ncRNA of AZIN1 in KIRC namely STK4‐AS1/AC068338.2‐miR‐106b‐5p‐AZIN1 axis as well as the downstream signaling pathways.Conclusion: This study illustrated the unique anti‐tumor role of AZIN1 in KIRC and provided potential value for guiding immunotherapy and targeted therapy.

In this research, a new method was developed for extracting fibre directly from the ramie. High-speed water treatment was found to be beneficial for the removal of husks. The proposed fibre extraction process included retting, first high-speed water treatment, oxidative degumming and second high-speed water treatment. The retting time and hydrogen peroxide concentration suitable for the removal of husks were found to be 7 days and 8 g/L, respectively. There was very little residual husk in the extracted sample. Scanning electron microscopy showed that most of the gums were removed. The fineness and tenacity values of the ramie fibre that was extracted using the proposed method were close to the values of the ramie fibre extracted by using the traditional process. As compared with the traditional process, the chemical agent consumption, boil time and labour intensity of the new extraction method were less.