Huifang Kuang

Objectives: Oral pain (OP) is a prevalent condition affecting one in four US adults, potentially influenced by diet through inflammation-related pathways. This study aimed to investigate the association between the Dietary Inflammatory Index (DII) and the prevalence of OP in a nationally representative sample.Methods: This cross-sectional study included 23,869 participants from the 2003–2018 National Health and Nutrition Examination Survey (NHANES). OP was assessed via a self-reported question regarding the experience of OP in the past year. DII scores were calculated using data from two 24-h dietary recall interviews. Weighted multivariable logistic regression models and subgroup analyses were performed to evaluate the relationship between DII and OP. Restricted cubic spline analysis was used to examine the shape of the association.Results: DII was positively associated with OP in the fully adjusted regression model (OR: 1.026, 95% CI: 1.001, 1.051). The association between DII and OP presented a U-shape, with a turning point of 0.95, indicating that both low and high levels of dietary inflammation were associated with an increased risk of OP. Subgroup analyses revealed no significant differences across different stratifications (p > 0.05 for all).Conclusion: A U-shaped association between dietary inflammatory potential and oral pain was identified in this nationally representative sample. Encouraging a balanced diet that avoids both pro-inflammatory and excessively anti-inflammatory extremes may serve as a preventive or therapeutic strategy to alleviate oral pain and improve overall oral health outcomes.

The chronic illness known as oral submucous fibrosis (OSF) results in tissue fibrosis, precancerous lesions, and scarring. It usually manifests itself in the buccal mucosa. It frequently occurs in the buccal mucosa. Von Hippel-Lindau (VHL) is an essential component of E3 ubiquitin ligase complex. The loss of VHL led to reduced fibrotic responses, accompanied by ameliorated fiber deposition. However, the precise impact of VHL on OSF is yet unclear. OSF tissues and normal mucosal tissues were applied to analyze the distinct expression of VHL and histone deacetylase 6 (HDAC6). Oral fibroblasts were treated to arecoline to simulate OSF in vitro, and molecular biological experiments were conducted to identify the role of VHL in buccal mucosa fibroblasts (BMFs). VHL was downregulated and HDAC6 was upregulated in OSF tissues and BMFs. Overexpression of VHL inhibited fibrosis in arecoline-treated BMFs. VHL inhibits the level of HDAC6 by inducing the ubiquitination of HDAC6. Knockdown of HDAC6 reduces the fibrogenic ability of BMFs. Furthermore, overexpression of HDAC6 contributes to the activation of NF-κB signaling in BMFs. HDAC6 selective inhibitor ACY-1215 inhibited the NF-κB signaling pathway. VHL attenuated arecoline-induced OSF by inhibiting the ubiquitination of HDAC6 and blocking NF-κB pathway. As a result, our study offers new perspectives into the discovery of novel tactics that can be employed against OSF.

Autogenous dentin matrix (ADM), derived from a patient’s extracted tooth, can be repurposed as an autologous grafting material in reconstructive dentistry. Extracted teeth provide a source for ADM, which distinguishes itself with its low rejection rate, osteoinductive capabilities and ease of preparation. Consequently, it presents a viable alternative to autogenous bone. Animal studies have substantiated its effective osteoinductive properties, while its clinical applications encompass post-extraction site preservation, maxillary sinus floor augmentation, and guided bone tissue regeneration. Nevertheless, the long-term efficacy of ADM applied in bone regeneration remains underexplored and there is a lack of standardization in the preparation processes. This paper comprehensively explores the composition, mechanisms underlying osteoinductivity, preparation methods, and clinical applications of ADM with the aim of establishing a fundamental reference for future studies on this subject.