Giuseppe Tafuri

Background: Thermal waters and peloid‐derived products have long been used in dermatological and spa treatments, although the cellular mechanisms underlying their biological effects remain incompletely characterized. This study investigated the effects of Saturnia spa water (TW) and an aqueous mud extract (MUD) on inflammatory, redox, and wound‐healing responses in human skin cell models. Methods: Human dermal fibroblasts (NHDF) and keratinocytes (HaCaT) were exposed to different concentrations of MUD and TW to assess cell viability by MTT assay. Based on the viability results, 5% MUD and TW were selected for subsequent experiments. TNF‐ α –stimulated cells were used to investigate inflammatory and oxidative responses by evaluating IL‐1 β and IL‐6 production, inflammatory and cytoprotective gene expression, reactive oxygen species (ROS), superoxide dismutase (SOD) activity, and glutathione (GSH) levels. A scratch assay was performed to evaluate the effects of the treatments on cell migration and wound repair. Results: MUD and TW showed no marked cytotoxic effects at concentrations up to 5% in either cell model, whereas the undiluted preparations affected cell viability. In TNF‐ α –stimulated NHDF cells, both MUD and TW significantly reduced IL‐1 β and IL‐6 gene expression ( p < 0.001), whereas MUD significantly reduced the secretion of both cytokines. Both treatments also enhanced HO‐1 expression and restored the TNF‐ α –induced reduction in SOD activity, whereas total ROS and GSH levels were not significantly altered. In HaCaT cells, TNF‐ α approximately doubled ROS levels ( p < 0.001), whereas both MUD and TW significantly reduced ROS production ( p < 0.01). TNF‐ α also reduced SOD activity by approximately 50% ( p < 0.001), an effect that was restored by both treatments. GSH levels remained unchanged. In the scratch assay, both extracts significantly promoted wound closure at 6 h in NHDF cells ( p = 0.002) and in HaCaT cells (MUD p = 0.017 and TW p = 0.027). Conclusions: Under the experimental conditions tested, Saturnia water and its aqueous mud extract exerted anti‐inflammatory and redox‐modulating effects in human keratinocytes and dermal fibroblasts, together with a positive effect on in vitro wound closure. These findings support further investigation of these spa‐derived preparations as potential complementary agents for skin repair and inflammatory modulation, although their disease‐specific effects require validation in more physiologically relevant models.

This study aims to analyze the precision and amount of dental tissue removed during overlay preparation by experienced and less experienced operators, with and without magnification systems. Methods: Sixty-four first upper molar Frasaco Typodonts were divided into four groups: experienced operators with magnification (Group 3, EXP+LOU), experienced operators without magnification (Group 1, EXP), dental students with magnification (Group 4, STU+LOU), and dental students without magnification (Group 2, STU). Preparations including an occlusal reduction of 1.5 mm and an interproximal box of 1 mm width. The prepared teeth were scanned and analyzed using reverse engineering software (Geomagic Control X, Oqton, San Francisco, CA, USA) to evaluate preparation accuracy, dental substance removal, and comparison to a control unprepared tooth. Results: The analysis showed that the average reduction was 32.19%. Group 4 (STU+LOU) showed significantly improved accuracy with magnification compared to Group 2 (STU). Group 3 (EXP+LOU) achieved the best result with an average reduction of 23.96%, while Group 2 (STU) had the worst result with 41.28%. Conclusions: Conservative indirect restorations, such as overlays, effectively preserve dental tissue. Operator experience and magnification systems are crucial for preparation accuracy. Magnification improves precision for less experienced operators, reducing tooth volume loss by 9.1%, and enhances cavity design accuracy, ensuring a better restoration fit and minimizing the marginal gap.

The precision of the luting protocol plays a crucial role in the success and survival rate of porcelain laminate veneers (PLVs). This in vitro study aimed to evaluate the influence of different luting techniques on the positioning of PLVs through a novel, noninvasive, scanning-based technique. A total of 45 ceramic PLVs were milled and cemented on human tooth replicas. Specimens were divided into three groups of 15, each subjected to a different luting protocol: flowable composite (Group A), dual-cure resin cement (Group B), and preheated composite resin (Group C). After luting procedures, specimens were scanned, and every STL file was superimposed with the original tooth design in Geomagic Control X version 2022.1 software to assess linear (incisal, mid, cervical) and angular discrepancies. Statistical analysis was performed using one-way ANOVA, Tukey’s HSD tests, and regression analysis. ANOVA results showed no statistically significant differences for incisal (F = 0.327, p = 0.723), mid (F = 0.287, p = 0.752), cervical (F = 0.191, p = 0.827), and angular (F = 0.026, p = 0.975) measurements. Tukey’s HSD post hoc tests confirmed the lack of significant pairwise differences between groups. The study demonstrated that the type of luting agent used does not significantly impact the final position of PLVs. This suggests flexibility in the choice of luting agents without compromising the accuracy of PLV placement.

Chlorhexidine digluconate (CHX) has shown the ability to significantly reduce inflammation and marginal bone loss (MBL) at the 1-year follow-up but limited data exist regarding its long-term efficacy in peri-implant stability. The objective was to compare the long-term effects (5 years of follow-up) of a placebo gel (16 patients in Group A) or a 0.20% CHX gel (15 patients in Group B) used at each previous surgical and prosthetic stage. Control visits were conducted in 2022, investigating the long-term effects by biological, radiological, and clinical evaluation. The data were statistically analyzed. The research achieved a 96.7% implant success rate over five years, but 41.9% of patients did not attend annual oral hygiene check-ups. The average MBL was 1.04 ± 0.39 mm, with no significant differences between the two groups. Notably, patients who attended regular periodontal check-ups experienced significantly less MBL than those who did not (p < 0.05). At five years, direct effects of CHX were absent, with both groups showing moderate bone loss. However, the results suggest that early disinfection could enhance both short- and long-term outcomes. In fact, patients with initial minor MBL due to use of CHX, preserve this advantage also after 5 years of follow-up. Additionally, the data underscore the importance of annual check-ups in early detection and management of biological complications.

Clinical outcomes of dental restorations depend primarily on the choice of materials used, and nowadays, dental CAD-CAM (Computer-Aided Design Computer-Aided Manufacturing) materials have strongly changed daily clinical practice. The aim of this systematic review is to analyze CAD-CAM dental materials according to their mechanical properties and in relation to their clinical applications. A literature review was performed on PubMed, Scopus, Web of Knowledge, and the Cochrane Library. Articles addressing at least one of the following topics regarding dental materials for CAD-CAM systems: manufacturers, mechanical features, materials’ composition, optical properties, clinical indications, and/or outcomes were included in the review. A flowchart was performed as described in the PRISMA guidelines. Among the 564 articles found, 63 were analyzed and evaluated. Within the limitations of this systematic review, it can be concluded that CAD-CAM materials present a wide range of clinical applications due to their improved mechanical properties. Specifically, in addition to materials that have been in use for a long time (such as feldspathic ceramics), resin block composites can also be used for permanent restorations.

Chlorhexidine digluconate (CHX) has shown the ability to significantly reduce inflammation and marginal bone loss (MBL) at 1-year follow-up but limited data exists regarding its long-term efficacy in peri implant stability. The objective was to compare the long-term effects of a placebo gel (16 patients in Group A) or a 0.20% CHX gel (15 patients in Group B) used at each previous surgical and prosthetic stage. Follow-ups were conducted in 2022, investigating by biological, radiological, clinical, mechanical evaluation the long-term effects. Data were statistically analyzed. The research achieved a 96.7% implant success rate over five years, but 41.9% of patients didn't attend annual oral hygiene check-ups. The average MBL was 1.04 ± 0.39 mm, with no significant differences between the two groups. Notably, patients who attended regular periodontal check-ups experienced significantly less MBL than those who didn't (p <0.05). At five years, the effects of CHX were reduced, with both groups showing moderate bone loss. The results suggest early disinfection could enhance both short and long-term outcomes. In fact, patient with initial minor MBL preserve this advantage also at 5 years of follow-up. Additionally, the data underscore the importance of annual check-ups in early detection and management of biological and mechanical complications.

Clinical outcomes of dental restorations depends primarily on the choice of materials used and their fabrication processes. The spectrum of dental CAD-CAM (Computer-Aided Design Computer-Aided Manufacturing) materials presents a wide range of variables on what may be the most suitable material for the clinical case. This narrative review focuses on CAD-CAM dental materials based on their mechanical properties and in relation to their clinical applications. A literature review was performed on PubMed, Scopus, Web of Knowledge, and Cochrane Library. Articles addressing at least one of the following topics regarding dental materials for CAD-CAM systems: manufacturers, mechanical features, materials ’composition optical properties, clinical indications and / or outcomes were included in the review. A flowchart was performed as de-scribed in the PRISMA guidelines. Among the 564 articles found, 63 were analyzed and evaluated. Within the limitation of this narrative review, it can be concluded that all CAD-CAM materials presents a wide range of clinical applications due to their improved mechanical properties. Specifically, in addition to materials that have been in use for a long time (such as feldspathic ceramics), resin block composites can also be used for permanent restorations.