Boris Vidal-Veuthey
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Research Interests
Boris Vidal-Veuthey has a total of 10 co-authors. Here are the top co-authors by number of shared publications:
- Alonso Ardiles-Candia
Centro de Bioinnovación Antofagasta (CBIA), Facultad de Ciencias del Mar y de Recursos Biológicos, Universidad de Antofagasta, Antofagasta 1270300, Chile
1 shared publication - Carlos Gatica-Cortes
Centro de Bioinnovación Antofagasta (CBIA), Facultad de Ciencias del Mar y de Recursos Biológicos, Universidad de Antofagasta, Antofagasta 1270300, Chile
1 shared publication - Carlos Riquelme
Centro de Bioinnovación Antofagasta (CBIA), Facultad de Ciencias del Mar y de Recursos Biológicos, Universidad de Antofagasta, Antofagasta 1270300, Chile
1 shared publication - José Luis Orrego Bartsch
Department of Food Science and Nutrition, Faculty of Health Sciences (FACSA), Universidad de Antofagasta, Angamos 601, Antofagasta 1240000, Chile
1 shared publication - Juan Pablo Cardenas
Departamento de Ciencias Biológicas, Facultad Ciencias de la Vida Universidad Andres Bello Santiago Chile
1 shared publication - Leonel Gonzalez
Centro de Bioinnovación Antofagasta (CBIA), Facultad de Ciencias del Mar y de Recursos Biológicos, Universidad de Antofagasta, Antofagasta 1270300, Chile
1 shared publication
Latest Publications
- Open Access
- 6 September 2024
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 25 (17) , 9642
Modification of the Trizol Method for the Extraction of RNA from Prorocentrum triestinum ACIZ_LEM2
In samples of harmful algal blooms (HABs), seawater can contain a high abundance of microorganisms and elemental ions. Along with the hardness of the walls of key HAB dinoflagellates such as Prorocentrum triestinum, this makes RNA extraction very difficult. These components interfere with RNA isolation, causing its degradation, in addition to the complex seawater properties of HABs that could hinder RNA isolation for effective RNA sequencing and transcriptome profiling. In this study, an RNA isolation technique was established through the modification of the Trizol method by applying the Micropestle System on cell pellets of P. triestinum frozen at −20 °C, obtained from 400 mL of culture with a total of 107 cells/mL. The results of the modified Trizol protocol generated quality RNA samples for transcriptomics sequencing, as determined by their measurement in Analyzer Agilent 4150.
- 6 September 2024
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 25 (17) , 9642
Open AccessModification of the Trizol Method for the Extraction of RNA from Prorocentrum triestinum ACIZ_LEM2
In samples of harmful algal blooms (HABs), seawater can contain a high abundance of microorganisms and elemental ions. Along with the hardness of the walls of key HAB dinoflagellates such as Prorocentrum triestinum, this makes RNA extraction very difficult. These components interfere with RNA isolation, causing its degradation, in addition to the complex seawater properties of HABs that could hinder RNA isolation for effective RNA sequencing and transcriptome profiling. In this study, an RNA isolation technique was established through the modification of the Trizol method by applying the Micropestle System on cell pellets of P. triestinum frozen at −20 °C, obtained from 400 mL of culture with a total of 107 cells/mL. The results of the modified Trizol protocol generated quality RNA samples for transcriptomics sequencing, as determined by their measurement in Analyzer Agilent 4150.