Rachel Kreis Douglas

Glomerella leaf spot (GLS) and bitter rot caused by species in the Colletotrichum gloeosporioides species complex are the most economically devastating fungal diseases of apples in North Carolina. Crop losses have reached 100% in highly susceptible cultivars. In the southeastern United States, management of this disease has predominantly relied on broad-spectrum fungicide programs targeting several summer diseases of apples. To improve control of GLS and bitter rot, field experiments to evaluate the efficacy of multi- and single-site fungicides were conducted over multiple years in a ‘Gala’ research orchard in Mills River, NC, from 2017 to 2019. Fungicides representing different modes of action were applied in a nonrotational program from petal fall until the immediate preharvest period to assess their efficacy in reducing GLS incidence, shoot defoliation caused by GLS, and pre- and postharvest bitter rot. Of the fungicides evaluated, Captan 80WDG (active ingredient [a.i.] captan), Merivon (a.i. fluxapyroxad and pyraclostrobin), Cabrio (a.i. pyraclostrobin), and Aprovia (a.i. benzovindiflupyr) provided good to excellent control against disease caused by Colletotrichum chrysophilum. In these programs, the incidence of GLS, expressed as the relative area under the disease progress curve, ranged from 4.6 to 43.8 in the Captan 80WDG (2019) and the Aprovia (2017) programs, respectively. The incidence of bitter rot at harvest in these programs from 2017 to 2019 ranged from 0.0 to 45.3% in the 2018 and 2017 Captan 80WDG programs, respectively. Conversely, the highest incidences of GLS and preharvest bitter rot were consistently observed in the Inspire (a.i. difenoconazole), Sercadis (a.i. fluxapyroxad), and untreated programs. Shoot defoliation owing to GLS followed a similar trend across the evaluated fungicide programs. In addition to nonrotational fungicide programs, the effect of carrier water pH for applications of captan was evaluated for bitter rot control in Virginia and North Carolina. No differences in captan efficacy were observed at either location. The results of this study will inform the development of new management programs for GLS and bitter rot in the southeastern United States and provide a foundation for the development of new season-long fungicide programs for apple disease management.

Glomerella leaf spot (GLS), Glomerella fruit rot (GFR), and apple bitter rot (ABR), caused by Colletotrichum spp., are among the most devastating apple diseases in the southeastern United States. Although several species have been identified as causal pathogens of GLS, GFR, and ABR, their relative frequency and fungicide sensitivity status in the southeastern United States is unknown. In total, 381 Colletotrichum isolates were obtained from symptomatic leaves and fruit from 18 conventionally managed apple orchards and two baseline populations in western North Carolina and Georgia in 2016 and 2017. Multilocus DNA sequence analysis revealed that C. chrysophilum was the predominant cause of GLS and GFR and C. fioriniae was the causal agent of ABR. Baseline and commercial populations of Colletotrichum spp. were evaluated for sensitivity to pyraclostrobin and trifloxystrobin, and no statistical differences in sensitivity between the two species were observed for conidial germination. However, the effective concentration that inhibited growth by 50% values were significantly lower for C. fioriniae compared with C. chrysophilum for both fungicides regarding mycelial inhibition. Isolates recovered from commercial orchards revealed that five populations of C. chrysophilum and one population of C. fioriniae had reduced sensitivity to trifloxystrobin, and one C. fioriniae population had reduced sensitivity to pyraclostrobin via conidial germination assays. The cytb gene for 27 isolates of C. fioriniae, C. chrysophilum, and C. fructicola with different quinone outside inhibitor (QoI) sensitivities revealed the G143A mutation in a single isolate of C. chrysophilum with insensitivity to both fungicides. The results of these studies suggest that two Colletotrichum spp. predominantly cause GLS and ABR in the southeastern United States and that a reduction in sensitivity to some QoI fungicides may be responsible for control failures. This study also provides a basis for monitoring shifts in QoI sensitivity in Colletotrichum spp. causing disease on apple in the southeastern United States.