Dinny Stevens

This study addresses current gaps in the 3D printing literature for filament composition (polymer type, color, and brand), aerosol emissions, and human health risks. Six metals (aluminum, magnesium, manganese, chromium, iron, and copper) were found in nonmetal filaments depending on polymer type, color, and brand using inductively coupled plasma-mass spectrometry. For copper- and steel-filled filaments, scanning electron microscopy and elemental analysis indicated a higher metal concentration within the filaments than on the surface. More VOCs were emitted from acrylonitrile butadiene styrene (ABS) filaments compared to polylactic acid (PLA) filaments, according to thermal desorption unit samples analyzed by gas chromatography. Styrene, emitted from ABS filaments, appears to pose the greatest potential health risk among known VOCs, given its concentration and reported effects. Toluene and benzaldehyde were emitted in lower concentrations from both ABS and PLA filaments and may pose a potential risk to human health. Based on three inflammatory markers across two exposure times using BEAS-2B epithelial lung cells, steel showed the highest proinflammatory response, possibly due to chromium and overall particulate matter. 3D printer users should minimize aerosolized emissions by taking proper precautions, such as ensuring adequate ventilation and wearing face masks, particularly during extended exposure.

Nanoparticles are proposed as alternatives to traditional antimicrobial agents. By manipulating a nanoparticle’s core and surface coating, antimicrobial effects against various microbial populations can be customized, known as the “designer effect”. However, the antimicrobial properties of nanoparticle core–coating combinations are understudied; little research exists on their effects on diverse bacteria. The antimicrobial effects of surface-stabilized zero-valent iron nanoparticles (FeNPs) are particularly interesting due to their stability in water and ferromagnetic properties. This study explores the impact of FeNPs coated with three surface coatings on six diverse bacterial species. The FeNPs were synthesized and capped with L-ascorbic acid (AA), cetyltrimethylammonium bromide (CTAB), or polyvinylpyrrolidone (PVP) using a bottom-up approach. Zone of inhibition (ZOI) values, assessed through the disc diffusion assay, indicated that AA-FeNPs and CTAB-FeNPs displayed the most potent antibacterial activity. Bacteria inhibition results ranked from most sensitive to least sensitive are the following: Bacillus nealsonii > Escherichia coli > Staphylococcus aureus > Delftia acidovorans > Chryseobacterium sp. > Sphingobacterium multivorum. Comparisons using ordinal regression and generalized linear mixed models revealed significant differences in bacterial responses to the different coatings and nanoparticle concentrations. The statistical model results are in agreement, thus increasing confidence in these conclusions. This study supports the feasibility of the “designer nanoparticle” concept and offers a framework for future research.

Engineered nanoparticles are precisely synthesized to exploit unique properties conferred by their small size and high surface area for environmental, biomedical, and agricultural applications. While these physical properties dictate functionality, they can also have various intended and unintended implications for biological systems. Both the particle size and shape influence cellular uptake. Because of zinc's antibacterial properties and role as a plant micronutrient, polyvinylpyrrolidone stabilized zinc nanoparticles (ZnNP) were selected for this study. Four synthesis methods were tested to produce distinct size populations of polymer-coated ZnNP, and all utilized water as the solvent to promote sustainable, green chemistry. The antibacterial activity of ZnNP was assessed in two agriculturally relevant bacteria strains: Escherichia coli and Bacillus cereus. To further examine the effects of ZnNP on bacterial cells, reactive oxygen species (ROS) generation was measured via hydrogen peroxide (H₂O₂) production. The bacteria's incubation temperature was also altered to assess bacterial growth and susceptibility after exposure to ZnNP. The ZnNP from the smaller size population inhibited the most growth across bacterial strains, assays, and incubation temperatures. Increased antibacterial effects and ROS production were observed after incubation at a higher temperature. These results indicate that the deliberately designed nanoparticles are potentially valuable in microbial control and offer promising solutions for the future of healthy agricultural systems.

Silver and copper nanoparticles (AgNPs and CuNPs) coated with stabilizing moieties induce oxidative stress in both bacteria and mammalian cells. Effective antibacterial agents that can overcome existing mechanisms of antibacterial resistance will greatly improve biomedical interventions. In this study, we analyzed the effect of nanoparticle-induced stress. Escherichia coli and normal human bronchial epithelial (BEAS-2B) cells were selected for this study. The nanoparticle constructs tested showed low toxicity to mammalian cells except for the polyvinylpyrrolidone-surface-stabilized copper nanoparticles. In fact, both types of copper nanoparticles used in this study induced higher levels of reactive oxygen species than the surface-stabilized silver nanoparticles. In contrast to mammalian cells, the surface-stabilized silver and copper nanoparticles showed varying levels of toxicity to bacteria cells. These data are expected to aid in bridging the knowledge gap in differential toxicities of silver and copper nanoparticles against bacteria and mammalian cells and will also improve infection interventions.