Tshephang Iris Kabelo
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Tshephang Iris Kabelo has a total of 6 co-authors. Here are the top co-authors by number of shared publications:
- Elliot Mpolokang Fana
World Organisation for Animal Health (WOAH) Foot-and-Mouth Disease Reference Laboratory, Botswana Vaccine Institute, Private Bag 0031, Gaborone, Botswana
1 shared publication - Joseph Hyera
World Organisation for Animal Health (WOAH) Foot-and-Mouth Disease Reference Laboratory, Botswana Vaccine Institute, Private Bag 0031, Gaborone, Botswana
1 shared publication - Kabo Masisi
Department of Biological Sciences and Biotechnology, School of Life Sciences, Botswana International University of Science and Technology, Private Bag 16, Palapye 1234, Botswana
1 shared publication - Kebaneilwe Lebani
Department of Biological Sciences and Biotechnology, Faculty of Science, Botswana International University of Science and Technology
1 shared publication - Kitsiso Gaboiphiwe
World Organisation for Animal Health (WOAH) Foot-and-Mouth Disease Reference Laboratory, Botswana Vaccine Institute, Private Bag 0031, Gaborone, Botswana
1 shared publication - Petronella Thato Mosholombe
Department of Biological Sciences and Biotechnology, School of Life Sciences, Botswana International University of Science and Technology, Private Bag 16, Palapye 10071, Botswana
1 shared publication
Latest Publications
- Open Access
A Review of the Utility of Established Cell Lines for Isolation and Propagation of the Southern African Territories Serotypes of Foot-and-Mouth Disease Virus
Cell culture underpins virus isolation and virus neutralisation tests, which are both gold-standard diagnostic methods for foot-and-mouth disease (FMD). Cell culture is also crucial for the propagation of inactivated foot-and-mouth disease virus (FMDV) vaccines. Both primary cells and cell lines are utilised in FMDV isolation and propagation. Widely used cell lines for FMDV and isolation and propagation include baby hamster kidney cells (BHK-21), swine kidney cells (IB-RS-2), foetal goat tongue (ZZ-R 127), foetal porcine kidney cells (LFBKvB6), bovine kidney cells (BK), human telomerase reverse transcriptase bovine thyroid (hTERT-BTY) and porcine kidney-originating PK-15 or SK 6 cell lines. This review highlights how different receptors and molecules—integrins, heparan sulphate (HS), and the Jumonji C-domain containing Protein 6 (JMJD6)—found on the surface of different cell types contribute to differences experienced with susceptibility and sensitivity of the cells to infection with different serotypes of FMDV. This review specifically focuses on Southern African territory (SAT) serotypes, which are unique to the Southern African context and are often under-investigated in cell line development for FMDV isolation and propagation.
Open AccessA Review of the Utility of Established Cell Lines for Isolation and Propagation of the Southern African Territories Serotypes of Foot-and-Mouth Disease Virus
Cell culture underpins virus isolation and virus neutralisation tests, which are both gold-standard diagnostic methods for foot-and-mouth disease (FMD). Cell culture is also crucial for the propagation of inactivated foot-and-mouth disease virus (FMDV) vaccines. Both primary cells and cell lines are utilised in FMDV isolation and propagation. Widely used cell lines for FMDV and isolation and propagation include baby hamster kidney cells (BHK-21), swine kidney cells (IB-RS-2), foetal goat tongue (ZZ-R 127), foetal porcine kidney cells (LFBKvB6), bovine kidney cells (BK), human telomerase reverse transcriptase bovine thyroid (hTERT-BTY) and porcine kidney-originating PK-15 or SK 6 cell lines. This review highlights how different receptors and molecules—integrins, heparan sulphate (HS), and the Jumonji C-domain containing Protein 6 (JMJD6)—found on the surface of different cell types contribute to differences experienced with susceptibility and sensitivity of the cells to infection with different serotypes of FMDV. This review specifically focuses on Southern African territory (SAT) serotypes, which are unique to the Southern African context and are often under-investigated in cell line development for FMDV isolation and propagation.