Mario Kokichi Uehara
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Mario Kokichi Uehara has a total of 11 co-authors. Here are the top co-authors by number of shared publications:
- Hiroyuki Ijima
Department of Chemical Engineering, Faculty of Engineering, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan
2 shared publications - Hiroshi Mizumoto
Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi Ward, Fukuoka 819-0395, Japan
1 shared publication - Kozue Yoshida
Department of Chemical Engineering, Faculty of Engineering, Graduate School, Kyushu University, Fukuoka, Japan
1 shared publication - Lucija Stefan
Department of Chemical Engineering, Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi Ward, Fukuoka 819-0395, Japan
1 shared publication - Muhammad Shafiq
Innovation Center of NanoMedicine (iCONM), Kawasaki Institute of Industrial Promotion, Kawasaki-ku, Kawasaki, 210-0821, Japan
1 shared publication - Nana Shirakigawa
Department of Chemical Engineering, Faculty of Engineering, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan
1 shared publication
Latest Publications
- 15 July 2025
- Published by Elsevier in Journal of Bioscience and Bioengineering
- Vol. 140 (4) , 244-251
Early-stage transplant responses in miniature-liver implants based on decellularization-recellularization technology
- 15 July 2025
- Published by Elsevier in Journal of Bioscience and Bioengineering
- Vol. 140 (4) , 244-251
Early-stage transplant responses in miniature-liver implants based on decellularization-recellularization technology
- 30 December 2024
- Published by Springer Nature in Cytotechnology
- Vol. 77 (1) , 30
Proposal for a non-adhesive single-cell culture technology for primary hepatocytes
Primary hepatocytes (PHs) are indispensable for studying liver function, drug screening, and regenerative medicine. However, freshly isolated PHs only survive for a few hours in non-adherent suspension culture. This study proposes treatment with PEG-GRGDS, a polymer-peptide conjugate comprising polyethylene glycol (PEG) and the pentapeptide sequence Gly-Arg-Gly-Asp-Ser (GRGDS), to sustain the viability of dispersed single PHs under non-adherent conditions. As a proof of concept, PHs treated with the PEG-GRGDS molecule were cultured in a microarray with single-cell-sized microwells. After 24 h of culture, enhanced cell survival was confirmed via esterase activity alongside activity for Cytochrome P450 1A1 (CYP1A1). Some liver-specific functionalities, including albumin secretion, were observed in the treated PHs. Additionally, it was observed that the length of the PEG-chain in the conjugates influenced the maintenance of single-cell dispersion and the levels of polymerized actin in the cells. These findings suggest that treatment with a polymer-peptide like PEG-GRGDS might provide a promising platform for the short-term culture of non-adherent single PHs.
- 30 December 2024
- Published by Springer Nature in Cytotechnology
- Vol. 77 (1) , 30
Proposal for a non-adhesive single-cell culture technology for primary hepatocytes
Primary hepatocytes (PHs) are indispensable for studying liver function, drug screening, and regenerative medicine. However, freshly isolated PHs only survive for a few hours in non-adherent suspension culture. This study proposes treatment with PEG-GRGDS, a polymer-peptide conjugate comprising polyethylene glycol (PEG) and the pentapeptide sequence Gly-Arg-Gly-Asp-Ser (GRGDS), to sustain the viability of dispersed single PHs under non-adherent conditions. As a proof of concept, PHs treated with the PEG-GRGDS molecule were cultured in a microarray with single-cell-sized microwells. After 24 h of culture, enhanced cell survival was confirmed via esterase activity alongside activity for Cytochrome P450 1A1 (CYP1A1). Some liver-specific functionalities, including albumin secretion, were observed in the treated PHs. Additionally, it was observed that the length of the PEG-chain in the conjugates influenced the maintenance of single-cell dispersion and the levels of polymerized actin in the cells. These findings suggest that treatment with a polymer-peptide like PEG-GRGDS might provide a promising platform for the short-term culture of non-adherent single PHs.