Rabeb Issaoui

Climate change, feed shortages, and rising production costs highlight the need for alternative and sustainable forages for ruminants. This study aimed to evaluate the nutritional composition, in vitro ruminal fermentation, and methane emissions of Urtica dioica ecotypes originating from contrasting bioclimatic zones in Tunisia. Aerial parts of Urtica dioica were harvested at the early flowering stage from arid, semi-arid, and sub-humid regions. Samples were subjected to chemical composition in vitro ruminal fermentation to determine dry matter degradability (DMD), neutral detergent fiber degradability (NDFD), metabolizable energy (ME), and methane production. The results demonstrate that Urtica dioica is a promising protein-rich forage, with a stable crude protein content across ecotypes (18.58–20.97% of dry matter). In contrast, NDFD, DMD, ME, and methane emissions varied significantly according to origin. The arid ecotype, characterized by the highest fiber, ether extract, and polyphenol content, exhibited the lowest DMD (53% vs. 61% and 60%), NDFD (45% vs. 55% and 56%), and ME (7.2 vs. 8.6 and 9.0 MJ/kg dry matter) but produced the lowest methane emissions (38.8 vs. 53.2 and 74.2 mL CH₄/kg DMD) compared with the semi-arid and sub-humid ecotypes. The semi-arid and sub-humid ecotypes had comparable DMD, NDFD, and ME values; however, methane emissions were higher in the sub-humid ecotype. Overall, the semi-arid ecotype provided the most favorable balance between nutritive quality and environmental sustainability. These findings highlight the critical role of ecological origin in determining the feeding value and greenhouse gas footprint of Urtica dioica, providing a scientific basis for its potential use as a sustainable forage in ruminant feeding systems.

Microplastic (MP) pollution is an emerging concern in ruminant production, as animals are exposed to MPs through air, water, and feeds. Ruminants play a key role in MP transmission to humans via animal products and contribute to MP return to agricultural soil through excreta. Identifying effective strategies to mitigate MP pollution in the ruminant sector is crucial. A promising yet understudied approach involves the potential ability of rumen microbiota to degrade MPs. This study investigated the in vitro ruminal degradation of three widely distributed MPs—low-density polyethylene (LDPE), polyethylene terephthalate (PET), and polyamide (PA)—over 24, 48, and 72 h. PET MP exhibited the highest degradation rates (24 h: 0.50 ± 0.070%; 48 h: 0.73 ± 0.057%; and 72 h: 0.96 ± 0.082%), followed by LDPE MP (24 h: 0.03 ± 0.020%; 48 h: 0.25 ± 0.053%; and 72 h: 0.56 ± 0.066%) and PA MP (24 h: 0.10 ± 0.045%; 48 h: 0.02 ± 0.015%; and 72 h: 0.14 ± 0.067%). These findings suggest that the ruminal environment could serve as a promising tool for LDPE, PET, and PA MPs degradation. Further research is needed to elucidate the mechanisms involved, potentially enhancing ruminants’ natural capacity to degrade MPs.

The increasing frequency of extreme weather events, exacerbated by climate change, has caused significant physical damage to crops worldwide. This study explores the potential of repurposing crop plants that exhibit structural breakage due to hailstorms and strong winds and were originally cultivated for seed production (amaranth, borage, camelina, flax, quinoa, soybean, and white lupin) as alternative forages for ruminants. Their nutritional value was assessed by analyzing chemical composition, in vitro dry matter degradability (DMD), in vitro neutral detergent fiber degradability (NDFD), estimated dry matter intake (DMI), and relative feed value (RFV) compared to conventional forages (alfalfa and ryegrass hay from undamaged plant). Results revealed significant variability among the damaged crops. Borage, amaranth, and white lupin exhibited superior DMD, NDFD, estimated DMI, and RFV, positioning them as promising forage alternatives. Soybean and quinoa showed protein content, DMD, NDFD, estimated DMI, and RFV comparable to alfalfa hay, suggesting their suitability as substitutes. However, camelina exhibited limited NDFD, while flax had low DMD, NDFD, estimated DMI, and RFV, indicating the need for pre-treatment strategies to optimize their nutritional value. Overall, repurposing weather-damaged borage, amaranth, white lupin, soybean, and quinoa as alternative forages for ruminants provides a promising approach to mitigating feed shortages, improving feed resource utilization, and optimizing resource utilization in livestock production.

Microplastics (MPs) have emerged as a significant environmental threat, infiltrating livestock systems. This study presents the first in vitro investigation of the effects of low-density polyethylene (LDPE) MP contamination on rumen fermentation dynamics and feed utilization in a simulated ruminal digestive system. Concentrate feed was incubated in buffered rumen fluid collected from lambs, supplemented with LDPE MPs at concentrations of 3.3 g/L and 6.6 g/L and compared to the concentrate incubated in the buffered rumen fluid without MP contamination. The results demonstrate that both levels of LDPE MPs significantly altered rumen fermentation dynamics by reducing asymptotic gas production by 11% and 15% and increasing the constant rate of gas production by 16% and 19% at low and high addition levels, respectively, compared to the control. However, the early-stage fermentation dynamics remained unaffected. Furthermore, both levels of LDPE MPs reduced rumen protozoal populations (20% and 23%) and ammonia-nitrogen levels by 11% at both of addition levels. Despite these disruptions, rumen pH remained unaffected. Increasing the addition level of LDPE from 3.3 to 6.6 g/L did not exacerbate the disruptions. The results of this study highlight the potential risks posed by LDPE MPs in ruminal nutrition. Further in vivo investigations are essential to validate these findings and assess their impact on animal performance.