Paulina Betancur Guerra

Aim: Feline mammary carcinoma (FMC) shares strong histological and molecular similarities with human breast cancer, facilitating elucidation of pathophysiological tumor mechanisms, identification of prognostic indicators, discovery of biomarkers and therapeutic targets. This study characterized the proteome of two FMC subtypes, aggressive cribiform (CRIB) and less aggressive tubulopapillary (TUB), using formalin-fixed, paraffin-embedded (FFPE) tissue samples to identify aggressive biomarkers.Main methods: Twenty-one FMC samples (CRIB, n = 12, TUB, n = 9) were retrieved from a histological biobank. Following deparaffinization and digestion, proteomes were analyzed by shotgun nLC-MS/MS proteomics, Data were evaluated by principal component analysis (PCA), dendrogram, partial least squares discriminant analysis (PLS-DA), VIP score and Volcano plot (FDR < 0.05). Venn diagrams, String DB and ShinyGO for pathway enrichment.Key findings: A mong 976 identified proteins,113 were differentially abundant between subtypes. PCA showed partial overlap, but 15 key proteins were identified, with 14 elevated in CRIB. CRIB-specific proteins included AHNAK nucleoprotein, glycerol kinase, myosin-2, and an uncharacterized protein, TUB-specific proteins included glucosylceramidase beta 3, β2-glycoprotein-1, SEA domain-containing protein, and EF-hand domain-containing protein. T-complex protein 1 subunit alpha and reticulon-3 (both human breast cancer poor prognosis markers) iwere common to both subtypes. Enriched pathways involved metabolic, immune, and signaling networks.Significance: FFPE-based proteomics effectively identifies subtype-specific biomarkers and pathways, offering translational insights into FMC and human breast cancer.

Synovitis is an inflammatory disorder in horses that contributes to cartilage degradation and osteoarthritis. This study evaluated the effects of intra-articular administration of high-molecular-weight (HMW), linear (non-cross-linked) hyaluronic acid (HA) in an equine model of lipopolysaccharide (LPS)-induced acute synovitis. In this randomized blinded study, acute synovitis was induced in radiocarpal joints of 16 adult horses using LPS (0.25 ng). After 12 h, joints were treated intra-articularly with either 2 mL of phosphate-buffered saline (PBS; control group) or 2 mL of HMW-HA (20 mg/mL; 2280 kDa; treatment group). Horses underwent orthopedic, ultrasonographic, and synovial fluid evaluations. Horses treated with HMW-HA exhibited lower synovial membrane thickening and reduced lameness scores compared with controls. Synovial fluid analysis demonstrated increased concentrations of HMW-HA during the acute inflammatory phase in treated joints. Reduced chondroitin sulfate release was also detected following HMW-HA administration. Lower lipid peroxidation levels (TBARS) were observed in the treated group at 24 h and 14 days. Intra-articular administration of high-molecular-weight linear hyaluronic acid was associated with increased synovial availability of HMW-HA during the acute inflammatory phase, attenuation of pain-related clinical signs and synovial membrane thickening, and modulation of the redox environment. These findings support a modulatory role of linear HMW-HA in the intra-articular environment.

BOTUCATU Jornada de Integração dos Alunos de Graduação e Pós-graduação – JOIA 2025 03 a 05 de novembro de 2025 A Jornada de Integração dos Alunos de Graduação e Pós-graduação (JOIA) teve sua primeira versão em 1997, coordenada e organizada pelo Prof. Sony Dimas Bicudo, do Departamento de Reprodução Animal e Radiologia Veterinária. Inicialmente denominada Jornada de Integração dos Alunos de Graduação e Pós-graduação em Reprodução Animal, passou a se chamar Jornada de Integração dos Alunos de Graduação e Pós-graduação, totalizando nove edições até 2005. Em 2018, com o propósito de resgatar a tradição do evento, a X edição foi coordenada e organizada pela Profª Fabiana Ferreira de Souza, a qual participou da primeira JOIA ainda como estudante e sempre reconheceu a importância da divulgação científica e da integração acadêmica, de alunos da pós-graduação, graduação e ensino médio, proporcionadas pela jornada. Sob sua liderança e dedicação, a JOIA foi realizada de forma contínua da edição X (2018) até a edição XVI (2024). Nesse período, mesmo diante de desafios como a pandemia, o evento manteve sua continuidade: em 2020 e 2021 em formato online, e a partir de 2022 retomando as atividades presenciais. Em 2023, a XV JOIA também celebrou os 10 anos do Programa de Pós-graduação em Biotecnologia Animal da FMVZ – UNESP Botucatu. Neste ano, a professora encerra seu ciclo na coordenação do evento, mas seguirá apoiando e participando ativamente, deixando como legado um exemplo de incentivo, inspiração e compromisso com a ciência.

The role of leukocytes in semen remains controversial, with both beneficial effects, such as the removal of microorganisms and abnormal sperm, and harmful effects, including reactive oxygen species (ROS) production and reduced fertility potential. Research on this topic in farm animals is limited. This study investigated correlations between differential leukocyte counts in bull semen and sperm parameters, both fresh and post-thaw. A total of 44 ejaculates from 36 adult bulls were collected using the artificial vagina method. Semen cytology was performed with Diff-Quick staining, and leukocytes were quantified under an optical microscope. Sperm parameters analyzed included ejaculate volume, motility (fresh and post-thaw), concentration, and morphology (major, minor, and total defects). Lymphocytes predominated (82%), followed by macrophages (13%), neutrophils (4%), and eosinophils (1%). Neutrophils showed weak negative correlations with major defects (r = -0.36, P = 0.027) and total defects (r = -0.328, P = 0.047), as well as a moderate negative correlation with post-thaw motility (r = -0.515, P = 0.0012) in Spearman’s correlation analysis. In contrast, lymphocytes, macrophages, and eosinophils showed no significant correlations with sperm parameters. These findings suggest that among leukocyte populations, only neutrophils negatively impact sperm morphology in fresh semen and motility in thawed semen.