Martina Gerli
Research Subjects
Martina Gerli has a total of 6 co-authors. Here are the top co-authors by number of shared publications:
- Alice Gualerzi
Fondazione Don Carlo Gnocchi Onlus
1 shared publication - Anna Teresa Brini
Department of Biomedical, Surgical and Dental Sciences, One Health, University of Milan, Milan, Italy, unimi.it
1 shared publication - Chiara Giannasi
Laboratory of Biotechnological Applications, IRCCS Istituto Ortopedico Galeazzi, 20157 Milan, Italy
1 shared publication - Elena Della Morte
Laboratory of Biotechnological Applications, IRCCS Istituto Ortopedico Galeazzi, 20157 Milan, Italy
1 shared publication - Francesca Cadelano
Laboratory of Biotechnological Applications, IRCCS Istituto Ortopedico Galeazzi, 20157 Milan, Italy
1 shared publication - Stefania Niada
Laboratory of Biotechnological Applications, IRCCS Istituto Ortopedico Galeazzi, 20157 Milan, Italy
1 shared publication
Latest Publications
- Open Access
Pre-Concentration Freezing Alters the Composition of Mesenchymal Stem/Stromal Cell-Conditioned Medium
Batch-to-batch reproducibility and robust quality assessment are crucial for producing cell-free biologics, such as conditioned medium (CM) derived from mesenchymal stem/stromal cells (MSCs). This study investigated the effects of freezing CM at −80 °C prior to concentration, a step that could occur in large scale pipelines, compared to freshly processed CM. Quality assessment included total protein quantification; extracellular vesicle evaluation using nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), and cytofluorimetry; and biochemical analysis using Raman spectroscopy. The freezing process resulted in a 34% reduction in total protein content, as confirmed for selected bioactive mediators, and significant depletion of specific particle types, particularly larger ones. Interestingly, the total particle concentration and polydispersity remained stable. Alterations in Raman spectra highlighted changes in protein, lipid, and nucleic acid content. These findings demonstrate that even routine steps like freezing can alter CM composition, likely due to temperature-induced structural changes in biological molecules. Careful consideration of pre- and intra-processing handling temperatures is critical for preserving the integrity of CM and ensuring consistent quality. This study emphasizes the importance of refining manufacturing protocols in the production of cell-free biologics.
Open AccessPre-Concentration Freezing Alters the Composition of Mesenchymal Stem/Stromal Cell-Conditioned Medium
Batch-to-batch reproducibility and robust quality assessment are crucial for producing cell-free biologics, such as conditioned medium (CM) derived from mesenchymal stem/stromal cells (MSCs). This study investigated the effects of freezing CM at −80 °C prior to concentration, a step that could occur in large scale pipelines, compared to freshly processed CM. Quality assessment included total protein quantification; extracellular vesicle evaluation using nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), and cytofluorimetry; and biochemical analysis using Raman spectroscopy. The freezing process resulted in a 34% reduction in total protein content, as confirmed for selected bioactive mediators, and significant depletion of specific particle types, particularly larger ones. Interestingly, the total particle concentration and polydispersity remained stable. Alterations in Raman spectra highlighted changes in protein, lipid, and nucleic acid content. These findings demonstrate that even routine steps like freezing can alter CM composition, likely due to temperature-induced structural changes in biological molecules. Careful consideration of pre- and intra-processing handling temperatures is critical for preserving the integrity of CM and ensuring consistent quality. This study emphasizes the importance of refining manufacturing protocols in the production of cell-free biologics.