Dong Wang

CD99 expression in H3K27M mutant and H3-WT expressing patient DMG tumors, tumor cell lines, and multiple normal tissues. Expression and functional diversity of CD99 isoforms across cellular contexts.

Systemic 10D1 animal treatment protocol, changes in tumor burden in the mouse pons with 10D1 treatment using different BLI images, changes in animal body weight. IHC images of the tumor tissue collected at the animal endpoint show CD99 and caspase 3 changes.

Western blot and flow cytometric data showing modulation of H3K27M mutation leading to changes in CD99 and H3K27me3 proteins in neoplastic and normal cells.

Fold changes in the RNA transcripts in SU-DIPG04 cells shNull vs shCD99 #1.

Selection and affinity of best binding CD99 antibody clones using western blotting and functional effects of 10D1 in DMG, normal human astrocytes, and iPScs.

Animal survival benefits after receiving either intravenous (IV), intrathecal (IT), or IT followed by IV delivery of 10D1 treatment as a monotherapy and when combined with FFRT.

Treatment protocol to evaluate the efficacy of 10D1 delivery routes combined with FFRT in DMG Xenografts. Changes in the tumor burden following the treatment protocol, shown by BLI images, and a significant increase in animal survival, demonstrated by Kaplan-Meier plot.

RNA qRT-PCR primers and their sequences.

Validation of depletion of CD99 in DMG cells by Western blotting and flow cytometry. Evaluation of functional effects of CD99-depleted cells, using cell proliferation assays, stemness by Aldefluor assay, differentiation by qRT-PCR, in vivo tumor growth inhibition, and apoptotic and differentiation markers.

Information about normal and neoplastic tissues and cell lines, including their source, growth media, genomic characteristics, and STR profiles.