Zhihui Cai

To investigate the protective effect of ammonium trichloride tellurate (AS101) on doxorubicin induced ovarian function damage in rats. Eighteen female SD rats were randomly divided into three groups with 6 rats in each group: group A blank control group (0.9% normal saline 0.1 ml intraperitoneally for 21 consecutive days), group B (0.9% normal saline 0.1 ml intraperitoneally for 5 days, doxorubicin 10 mg / kg intraperitoneally on day 6, and continued to be injected with normal saline for 15 days), group C (0.9% normal saline 0.1 ml intraperitoneally for 5 days, doxorubicin 10 mg / kg intraperitoneally on day 6, and continued to be injected with AS101 twice every other day). The contents to be recorded by the operator team include: (1) before operation: ① observe the general state of rats in each group and record the weight of rats. ② Vaginal smears of rats in each group were observed at the same time every day to observe the estrous cycle of rats. (2) After operation: ① observe the general state of rats in each group, read the weight of rats, and record the data Vaginal smears of rats in each group were observed at the same time every day after operation to observe the estrous cycle of rats. ③ At the end of the postoperative observation period, blood samples were taken from the tail of rats to measure the serum levels of estrogen 2 (E2), follicle stimulating hormone (FSH), and anti-M ü llerian hormone (AMH). ④ At the end of the observation period, the animals were sacrificed to observe the development of bilateral ovarian follicles. ⑤ At the end of the observation period, blood was taken from the abdominal aorta of rats after anesthesia, and the upper serum was separated after centrifugation at 3000 rpm. The levels of malondialdehyde (MDA), superoxide dismutase (SOD) and reactive oxygen species (ROS) were measured according to the operation methods of the kit instructions. The body weight of rats in group B was significantly lower than that in group A, while that in group C was significantly higher than that in group B (P < 0.05). The estrous cycle of rats in group B was disordered, and the estrous cycle of rats in group C was restored regularly (P < 0.05). The serum estradiol(E2) and AMH levels in group C were significantly higher than those in group B, and the FSH level was significantly lower than that in group B. Compared with group B, the number of primary follicles increased and the number of atretic follicles decreased in group C (P < 0.05). The SOD level in group B was lower than that in group A, and that in group C was higher than that in group B (P < 0.05). The levels of MDA and ROS in group B were higher than those in group A (P < 0.05), while those in group C were lower than those in group B (P < 0.05). AS101 can protect the damage of doxorubicin on ovarian function in rats, and its mechanism is related to its antioxidant effect. Not applicable.

In this study, ovarian transplantation at different sites was performed on the rat model of premature ovarian failure, to explore the effect on the recovery of ovarian function, and to evaluate the application value in the treatment of ovarian function decline caused by the application of chemotherapy drugs. Thirty 2-month-old female SD rats of SPF grade were randomly divided into five groups, and both ovaries in the model group were treated with cisplatin (11 mg/kg) for modelling. After successful modelling, another 18 rats were taken as donor rats: group A was the control group, group B was the model group and group CDE was the experimental group. Ovary transplantation was performed using muscle, subcutaneous and renal peritoneum, respectively, and the rats were monitored for their general condition, estrous cycle and serum hormone levels after surgery. At the end of the observation period, the rats were executed and follicular development was observed.We confirm that all methods are carried out in accordance with the relevant guidelines and regulations.After 14 days of xenotransplantation, the general condition of the rats in the experimental group was better than that of the model group. Serum E2 and AMH levels were significantly higher than those in the model group (P = 0.000), and FSH levels were significantly lower than those in the control group (P = 0.000). Cisplatin can be used to successfully establish the rat POF model; the three transplantation sites (muscle, subcutaneous and renal peritoneum) selected in this experiment can make the transplanted ovarian tissue survive and successfully restore the endocrine function of the body, and the effect of which showed no significant difference.