Jhon Edler Lopez-Merino

Adventitious rooting remains a major constraint for the clonal propagation of Coffea arabica, limiting the large-scale multiplication of elite genotypes. This study evaluated the effects of genotype, auxin treatment, and their interaction on adventitious rooting and basal callus formation in coffee cuttings under controlled nursery conditions, while morphophysiological traits were assessed as complementary indicators of cutting performance. A completely randomized 3 × 3 factorial design was used, including three hybrids (H3, Excelencia, and Milenio) and three plant growth regulator (PGR) treatments: control, indole-3-butyric acid (IBA), and a commercial auxin formulation (RH; NAA + IBA). Rooting probability and root number were significantly affected by PGR treatment, whereas the longest root length was influenced by hybrid, PGR treatment, and their interaction. Model-estimated rooting probability increased from 4.76% in the control to 17.05% under IBA and 35.73% under RH. Similarly, the estimated number of roots per cutting increased from 0.14 in the control to 0.99 under IBA and 1.85 under RH. Although the hybrid × PGR interaction was not significant for rooting probability, the highest observed rooting percentage was recorded in H3 under RH (52.38%), followed by Milenio under RH (33.33%). For the longest root length, the strongest responses were observed under RH, particularly in Milenio (71.15 mm) and H3 (70.08 mm). Callus formation varied among treatments, but its association with rooting performance was weak and inconsistent. Morphophysiological traits provided complementary information on cutting status but were not interpreted as direct mechanistic drivers of rooting. These findings indicate that adventitious rooting in C. arabica was more closely associated with genotype-dependent responsiveness to exogenous auxin than with the extent of callus formation. However, anatomical studies are needed to determine the developmental origin of root primordia and their possible relationship with callus tissue.

Coffee pulp, a by-product of coffee processing and a rich source of bioactive compounds, is a promising raw material for functional beverages. However, the influence of genetic variability among coffee varieties on the functional and sensory properties of pulp infusions remains poorly understood. This study evaluated physicochemical, antioxidant, and sensory properties of infusions from nine Coffea arabica L. varieties. Significant differences among varieties were observed (p < 0.05). The pH ranged from 5.36 to 6.42, and titratable acidity from 0.06 to 0.08 g/100 mL, indicating a mild acidic profile. Antioxidant activity (DPPH) ranged from 460.52 to 1006.03 µmol TE/L, and total phenolic content from 29.47 to 59.27 mg GAE/L. Geisha showed the highest antioxidant activity and phenolic content, while Casiopea exhibited the highest reducing capacity. In contrast, Oro Azteca, Excelencia, and H1 achieved the highest sensory acceptance. Multivariate analysis confirmed clear differentiation among varieties and a separation between bioactive and sensory-related attributes. These findings highlight the role of varietal selection in balancing functional potential and consumer acceptance, supporting the development of functional beverages within a circular economy framework.

Coffee leaf rust (Hemileia vastatrix) is a major constraint on coffee production, while reliance on chemical fungicides raises environmental concerns and may become less sustainable over time. This review critically analyzes the available evidence on the potential of Lecanicillium uredinophilum as a biological control agent against H. vastatrix, with comparative consideration of other microbial agents evaluated for coffee leaf rust management. A structured literature review was conducted using searches in Scopus and PubMed, complemented by manual searches and reference screening. The available evidence indicates that L. uredinophilum shows affinity for urediniospore structures and exhibits mycoparasitic activity against rust fungi under controlled conditions. However, direct evidence against H. vastatrix remains limited and is still concentrated mainly in laboratory and greenhouse studies. In comparison with more established biocontrol agents, L. uredinophilum should be regarded as a promising but still early-stage candidate whose practical relevance has not yet been validated. Future progress will depend on robust field-based studies, improved understanding of its mechanisms of action, evaluation of its environmental stability, and the development of viable formulations compatible with integrated disease management strategies.

Drought events intensified by climate change severely compromise the physiological stability and productivity of Coffea arabica, particularly in rainfed systems, underscoring the need to identify cultivars with greater functional resilience. This study evaluated the physiological, nutritional and biochemical responses of seedlings from ten cultivars subjected to adequate irrigation (AW), severe water deficit (SWD) and rehydration (RI). Water potential, gas exchange, oxidative stress markers, stomatal traits and foliar macro- and micronutrients were quantified. Most cultivars exhibited pronounced reductions in the pre-dawn leaf water potential (Ψ_pd), photosynthesis (A), stomatal conductance (g_s) and transpiration (E), together with increases in oxidative stress indicators under SWD. In contrast, Obatá amarillo, Castillo, and Arará maintained greater hydraulic stability, more efficient stomatal regulation, higher water-use efficiency, and lower oxidative stress, accompanied by a more effective post-stress recovery after RI. Regarding nutrient dynamics, Geisha, Castillo, and Arará showed higher K⁺ accumulation, while Catimor bolo presented elevated Ca²⁺, P, and Fe²⁺ contents, elements associated with metabolic reactivation and structural recovery after stress. Geisha and Marsellesa displayed an adaptive, recovery-driven resilience strategy following drought stress. Overall, the findings identify Obatá amarillo, Castillo, and Arará as the most drought-tolerant cultivars, highlighting their potential relevance for breeding programs aimed at improving drought resilience in coffee.

Fusarium oxysporum f. sp. cubense (Foc) ha mostrado una alta virulencia y es responsable de pérdidas significativas en la productividad del cultivo de plátano. El objetivo de este estudio fue evaluar el potencial antifúngico de cepas endófitas de Trichoderma spp. contra Foc. La actividad antifúngica se determinó mediante el crecimiento micelial radial de la cepa Foc (MC3) en confrontación con tres cepas endófitas de Trichoderma (HSC-5, HSC-9 y HSC-10), mediante la siembra de discos con micelio opuesto en medio de cultivo PDA, incubados a 28 ºC durante 4 días. Los resultados obtenidos mostraron los mayores grados de antibiosis en la confrontación con la cepa endófita Trichoderma HSC-5 (66.9%), seguido por HSC-9 (65.1%) y HSC-10 (60.5%), respectivamente. Los resultados son prometedores para su uso en una estrategia de gestión integrada de Foc.