Sook Ling Lim
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Sook Ling Lim has a total of 10 co-authors. Here are the top co-authors by number of shared publications:
- Chui Pin Leaw
Institute of Ocean and Earth Sciences, University of Malaya, 16310 Bachok, Kelantan, Malaysia
1 shared publication - Kek Heng Chua
Department of Biomedical Science, Faculty of Medicine Universiti Malaya Kuala Lumpur Malaysia
1 shared publication - Kieng Soon Hii
Bachok Marine Research Station, Institute of Ocean and Earth Sciences, University of Malaya, Bachok 16310, Kelantan, Malaysia
1 shared publication - Kyaw Thu Aung
National Centre for Food Science, Singapore Food Agency, 7 International Business Park, Singapore 609919 Singapore; School of Biological Sciences, Nanyang Technological University, 60 Nanyang Dr, Singapore 637551 Singapore
1 shared publication - Nur Insyirah Mohd Razalan
Department of Biomedical Science, Faculty of Medicine, Universiti Malaya, Kuala Lumpur 50603, Malaysia
1 shared publication - Po Teen Lim
Bachok Marine Research Station, Institute of Ocean and Earth Sciences, University of Malaya, Bachok, Kelantan 16310, Malaysia
1 shared publication
Latest Publications
- Open Access
Bacterial Community Composition and Prevalence of Aeromonas dhakensis in Four Tilapia Freshwater Aquaculture Systems in Malaysia
The tilapia (Oreochromis) aquaculture industry in Malaysia has expanded rapidly to meet the increasing demand for animal protein. However, this growth is challenged by microbial infections, particularly those caused by the emerging pathogen Aeromonas dhakensis. This study aimed to investigate the microbial community composition across four distinct tilapia farming systems and assess associated water physicochemical parameters, with a focus on detecting the presence of A. dhakensis and elucidating its environmental associations. Water physicochemical parameters were measured to evaluate environmental conditions, microbial communities were characterized through 16S rDNA metabarcoding, and A. dhakensis was detected using both microbiological and molecular approaches. Principal component analysis (PCA) and canonical correspondence analysis (CCA) were employed to explore the influence of environmental variables and microbial community dynamics on pathogen occurrence. Our results indicated that floating cages exhibited higher levels of temperature, ammonium, and fecal coliform, while cement tanks showed signs of nutrient accumulation. PCA revealed that both systems were associated with degraded water quality. A total of 45 A. dhakensis strains with distinct fingerprints were isolated. The 16S metabarcoding revealed Proteobacteria, Actinobacteria, and Planctomycetota as the dominant phyla. Alpha diversity did not differ significantly among pond systems, while beta diversity revealed variations in microbial assemblages across aquaculture systems. CCA identified dissolved oxygen, temperature, macronutrients (phosphate, ammonium, nitrate, and nitrite), and turbidity as significant environmental drivers shaping the microbial community structure across the pond systems. In conclusion, this study highlights the importance of environmental factors, particularly dissolved oxygen, temperature, and nutrient levels, in shaping microbial community composition and potentially influencing the presence of pathogenic bacteria such as A. dhakensis. These findings underscore the need for improved environmental management in tilapia aquaculture to mitigate disease risks and support fish health.
Open AccessBacterial Community Composition and Prevalence of Aeromonas dhakensis in Four Tilapia Freshwater Aquaculture Systems in Malaysia
The tilapia (Oreochromis) aquaculture industry in Malaysia has expanded rapidly to meet the increasing demand for animal protein. However, this growth is challenged by microbial infections, particularly those caused by the emerging pathogen Aeromonas dhakensis. This study aimed to investigate the microbial community composition across four distinct tilapia farming systems and assess associated water physicochemical parameters, with a focus on detecting the presence of A. dhakensis and elucidating its environmental associations. Water physicochemical parameters were measured to evaluate environmental conditions, microbial communities were characterized through 16S rDNA metabarcoding, and A. dhakensis was detected using both microbiological and molecular approaches. Principal component analysis (PCA) and canonical correspondence analysis (CCA) were employed to explore the influence of environmental variables and microbial community dynamics on pathogen occurrence. Our results indicated that floating cages exhibited higher levels of temperature, ammonium, and fecal coliform, while cement tanks showed signs of nutrient accumulation. PCA revealed that both systems were associated with degraded water quality. A total of 45 A. dhakensis strains with distinct fingerprints were isolated. The 16S metabarcoding revealed Proteobacteria, Actinobacteria, and Planctomycetota as the dominant phyla. Alpha diversity did not differ significantly among pond systems, while beta diversity revealed variations in microbial assemblages across aquaculture systems. CCA identified dissolved oxygen, temperature, macronutrients (phosphate, ammonium, nitrate, and nitrite), and turbidity as significant environmental drivers shaping the microbial community structure across the pond systems. In conclusion, this study highlights the importance of environmental factors, particularly dissolved oxygen, temperature, and nutrient levels, in shaping microbial community composition and potentially influencing the presence of pathogenic bacteria such as A. dhakensis. These findings underscore the need for improved environmental management in tilapia aquaculture to mitigate disease risks and support fish health.