Lyubov I. Kazakova
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Lyubov I. Kazakova has a total of 2 co-authors. Here are the top co-authors by number of shared publications:
- Gleb Borisovich Sukhorukov
Skolkovo Institute of Science and Technology
1 shared publication Lyudmila I. Shabarchina
Institute of Theoretical and Experimental Biophysics RAS, Institutskaya Street 3, 142290 Pushchino, Moscow region, Russia
1 shared publication
Latest Publications
- 13 May 2011
- Published by Royal Society of Chemistry (RSC) in Physical Chemistry Chemical Physics
- Vol. 13 (23) , 11110-11117
Co-encapsulation of enzyme and sensitive dye as a tool for fabrication of microcapsule based sensor for urea measuring
Enzyme based micron sized sensing system with optical readout was fabricated by co-encapsulation of urease and dextran couple with pH sensitive dye SNARF-1 into polyelectrolyte multilayer capsules. Co-precipitation of calcium carbonate, urease and dextran followed up by multilayer film coating and Ca-extracting by EDTA resulted in the formation of 3.5–4 micron capsules, what enable the calibrated fluorescence response to urea in concentration range from 10−6 to 10−1 M. The presence of urea can be monitored on a single capsule level as illustrated by confocal fluorescent microscopy. Variations in urease:dye ratio in capsules, applicability and limits of use of that type multi-component microencapsulated sensors are discussed.
- 13 May 2011
- Published by Royal Society of Chemistry (RSC) in Physical Chemistry Chemical Physics
- Vol. 13 (23) , 11110-11117
Co-encapsulation of enzyme and sensitive dye as a tool for fabrication of microcapsule based sensor for urea measuring
Enzyme based micron sized sensing system with optical readout was fabricated by co-encapsulation of urease and dextran couple with pH sensitive dye SNARF-1 into polyelectrolyte multilayer capsules. Co-precipitation of calcium carbonate, urease and dextran followed up by multilayer film coating and Ca-extracting by EDTA resulted in the formation of 3.5–4 micron capsules, what enable the calibrated fluorescence response to urea in concentration range from 10−6 to 10−1 M. The presence of urea can be monitored on a single capsule level as illustrated by confocal fluorescent microscopy. Variations in urease:dye ratio in capsules, applicability and limits of use of that type multi-component microencapsulated sensors are discussed.