Xiao-Zhen Jiang

We present a method based on quantum dot labeling technology for rapidly detecting the virulence of Helicobacter pylori and performing typing, aiming to overcome the limitations of conventional diagnostic methods and provide a basis for personalized clinical treatment. A kit was designed using the double-antigen sandwich method. This involved a series of meticulous steps, including establishing buffer reaction systems and conducting activation and coupling processes for antigen-antibody reactions during kit preparation. Additionally, there were specific procedures for sample collection, processing, and testing operations to determine the infection status and virulence type of Helicobacter pylori. By establishing data models for different results (negative, type II, and type I), the effectiveness of the quantum dot technology in typing detection was verified. In the case of negative samples, there were weak or no significant signal values in relevant detection areas. For type II samples, a specific pattern of positive and negative signal values for different antibodies was observed, and for type I samples, a distinct set of positive signal values corresponding to key antibodies was noted. Moreover, its application in studies related to chronic gastritis and gastric cancer demonstrated its value in serving as a screening indicator. The quantum dot-based method has advantages like improved sensitivity and specificity compared to traditional diagnostic methods. However, it also faces challenges such as potential impacts from sample storage time and relatively high costs. Nevertheless, with continuous technological innovations, it is expected to play a more significant role in the biomedical field for Helicobacter pylori detection and related disease diagnosis in the future.

We present a method based on quantum dot labeling technology for rapidly detecting the virulence of Helicobacter pylori and performing typing, aiming to overcome the limitations of conventional diagnostic methods and provide a basis for personalized clinical treatment. A kit was designed using the double-antigen sandwich method. This involved a series of meticulous steps, including establishing buffer reaction systems and conducting activation and coupling processes for antigen-antibody reactions during kit preparation. Additionally, there were specific procedures for sample collection, processing, and testing operations to determine the infection status and virulence type of Helicobacter pylori. By establishing data models for different results (negative, type II, and type I), the effectiveness of the quantum dot technology in typing detection was verified. In the case of negative samples, there were weak or no significant signal values in relevant detection areas. For type II samples, a specific pattern of positive and negative signal values for different antibodies was observed, and for type I samples, a distinct set of positive signal values corresponding to key antibodies was noted. Moreover, its application in studies related to chronic gastritis and gastric cancer demonstrated its value in serving as a screening indicator. The quantum dot-based method has advantages like improved sensitivity and specificity compared to traditional diagnostic methods. However, it also faces challenges such as potential impacts from sample storage time and relatively high costs. Nevertheless, with continuous technological innovations, it is expected to play a more significant role in the biomedical field for Helicobacter pylori detection and related disease diagnosis in the future.

Helicobacter pylori can be parasitic in gastric mucosa, which can cause a series of gastrointestinal diseases after infection and is closely related to gastritis, gastric ulcer, and gastric cancer. The high prevalence of H. pylori infection in regions with poor medical infrastructure and inadequate sanitation areas remains a significant public health concern. Consequently, the development of rapid, simple, and cost-effective screening methods for H. pylori detection in resource-limited settings is of paramount importance. In this study, we conducted a community-based screening in Shitan Village, Guangdong Province, a remote and underdeveloped area characterized by a permanent population of approximately 300 residents with generally low educational attainment. We employed a double antibody sandwich assay for the detection of H. pylori fecal antigen, a method chosen for its potential applicability in low-resource settings. A total of 261 participants from the village were enrolled, and their fecal samples were analyzed using this technique. For comparative validation, the same samples were subjected to quantitative polymerase chain reaction (qPCR) analysis. Compared with qPCR results, the sensitivity and specificity of the detection of H. pylori antigen in feces were 60.24% and 80.08%. The results demonstrated a strong agreement between the fecal antigen detection method and qPCR (Kappa = 0.630). This study systematically elucidated the principles, procedures, methodologies, and clinical applications of fecal antigen detection for H. pylori infection, aiming to explore latex-based double antibody sandwich technology and establish novel strategies and practical guidelines for its auxiliary diagnosis.

Helicobacter pylori can be parasitic in gastric mucosa, which can cause a series of gastrointestinal diseases after infection and is closely related to gastritis, gastric ulcer, and gastric cancer. The high prevalence of H. pylori infection in regions with poor medical infrastructure and inadequate sanitation areas remains a significant public health concern. Consequently, the development of rapid, simple, and cost-effective screening methods for H. pylori detection in resource-limited settings is of paramount importance. In this study, we conducted a community-based screening in Shitan Village, Guangdong Province, a remote and underdeveloped area characterized by a permanent population of approximately 300 residents with generally low educational attainment. We employed a double antibody sandwich assay for the detection of H. pylori fecal antigen, a method chosen for its potential applicability in low-resource settings. A total of 261 participants from the village were enrolled, and their fecal samples were analyzed using this technique. For comparative validation, the same samples were subjected to quantitative polymerase chain reaction (qPCR) analysis. Compared with qPCR results, the sensitivity and specificity of the detection of H. pylori antigen in feces were 60.24% and 80.08%. The results demonstrated a strong agreement between the fecal antigen detection method and qPCR (Kappa = 0.630). This study systematically elucidated the principles, procedures, methodologies, and clinical applications of fecal antigen detection for H. pylori infection, aiming to explore latex-based double antibody sandwich technology and establish novel strategies and practical guidelines for its auxiliary diagnosis.