Courage Siame

Breast cancer is the leading cause of cancer mortality among women globally, and drug resistance complicates treatment. Garlic-derived organosulfur compounds exhibit anticancer potential, but their multi-target activity against key breast cancer biomarkers remains unclear. This study utilized AutoDock Vina for molecular docking, OpenBabel for post-docking energy minimization, and employs SWISS-ADME and PreADMET platforms for ADMET profiling to assess six garlic compounds (Z-ajoene, allyl-methyl trisulfide, diallyl disulfide, diallyl sulfide, diallyl trisulfide, and S-allyl-L-cysteine) against clinically relevant breast cancer targets. Z-ajoene showed strong binding to Bcl-2, Topoisomerase II, and CDK-2, while S-allyl-L-cysteine targets five biomarkers. All compounds complied with Lipinski’s rule of five, indicating good oral bioavailability, and display favorable ADMET properties with no mutagenic or tumorigenic risks. Most compounds were predicted to inhibit P-glycoprotein, while only Z-ajoene showed potential inhibition of CYP2C9, suggesting possible drug-drug interactions. Despite moderate affinities, these compounds may serve as potential promising multi-target agents in breast cancer therapy. Our computational findings provide preliminary evidence that garlic-derived compounds warrant further in vitro and in vivo evaluation, particularly in the context of drug-resistant breast cancer.

Breast cancer is the leading cause of cancer mortality among women globally, and drug resistance complicates treatment. Garlic-derived organosulfur compounds exhibit anticancer potential, but their multi-target activity against key breast cancer biomarkers remains unclear. This study utilizes AutoDock Vina for molecular docking, OpenBabel for post-docking energy minimization, and employs SWISS-ADME and PreADMET platforms for ADMET profiling to assess six garlic compounds (Z-ajoene, allyl-methyl trisulfide, diallyl disulfide, diallyl sulfide, diallyl trisulfide, and S-allyl-L-cysteine) against clinically relevant breast cancer targets. Z-ajoene shows strong binding to Bcl-2, Topoisomerase II, and CDK-2, while S-allyl-L-cysteine targets five biomarkers. All compounds comply with Lipinski’s rule of five, indicating good oral bioavailability, and display favorable ADMET properties with no mutagenic or tumorigenic risks. Most compounds were predicted to inhibit P-glycoprotein, while only Z-ajoene showed potential inhibition of CYP2C9, suggesting possible drug-drug interactions. Despite moderate affinities, these compounds may serve as potential promising multi-target agents in breast cancer therapy. Our computational findings provide preliminary evidence that garlic-derived compounds warrant further in vitro and in vivo evaluation, particularly in the context of drug-resistant breast cancer.

Background/Objectives: Breast cancer incidence is increasing in younger Ghanaian women. However, few epidemiological studies have evaluated the modifiable risk factors in this population. Additionally, these studies have classified breast cancer in Ghanaian women based on the global menopausal case classification. This study reclassified premenopausal and postmenopausal breast cancer in a Ghanaian cohort, assessing the risk factors using the observed menopausal age in Ghanaian women of 48 years, rather than the global standard of 50 years. Methods: Women diagnosed with breast cancer and scheduled for surgery from December 2018 to March 2023 were recruited across four hospitals in Ghana for the Ghana Breast Cancer Omics Project (BCOPGh), and data were collected using a questionnaire. Cross-tabulation and linear regression were used to evaluate the relationships between categorical variables and age at diagnosis. Results: Out of a total of 262 women recruited, 34.4% were classified as having premenopausal breast cancer, while early-onset breast cancer (EOBC) accounted for 14.9% of all cases. The molecular subtypes were predominantly hormone receptor (HR)-positive (61%) while triple-negative breast cancer (TNBC) accounted for 16%. The tumours were predominantly at stage II (62%) and grade 2 (51%), with invasive carcinoma NST (56%) being the most common subtype. Within this cohort, nulliparity increased the odds of EOBC by 13.5-fold, while having a first birth after the age of 23 doubled the odds of premenopausal breast cancer. Reproductive factors (menarche and menopause) and lifestyle (alcohol intake, smoking, contraceptive use, and breastfeeding duration) were not associated with premenopausal breast cancer in this cohort. About 13% of participants reported a family history of breast cancer, and 79% had prior knowledge of the disease. Conclusion: This study supports previous reports of the relatively higher incidence of aggressive disease in young Ghanaian women and the protective effect of early age at first birth. It further underscores the need to investigate its genetic underpinnings, whilst highlighting the importance of public education on self-examination techniques to reduce advanced disease presentation in Ghanaian women.

Background/Objectives: Breast cancer incidence is increasing in younger Ghanaian women. However, few epidemiological studies have evaluated the modifiable risk factors in this population. Additionally, these studies have classified breast cancer in Ghanaian women based on the global menopausal case classification. This study reclassified premenopausal and postmenopausal breast cancer in a Ghanaian cohort, assessing the risk factors using the observed menopausal age in Ghanaian women of 48 years, rather than the global standard of 50 years. Methods: Women di-agnosed with breast cancer and scheduled for surgery from December 2018 to March 2023 were recruited across four hospitals in Ghana for the Ghana Breast Cancer Omics Project (BCOPGh), and data were collected using a questionnaire. Cross-tabulation and linear regression were used to evaluate the relationships between categorical variables and age at diagnosis. Results: Out of a total of 262 women recruited, 34.4% were classified as having premenopausal breast cancer; while early-onset breast cancer (EOBC) accounted for 14.9% of all cases. The molecular subtypes were predominantly Hormone receptor (HR)-positive (61%) while triple-negative breast cancer (TNBC) accounted for 16%. The tumours were predominantly at stage II (62%) and grade 2 (51%) with invasive carcinoma NST (56%) being the most common subtype. Within this cohort, nulliparity increased EOBC risk by 13.5-fold, while having a first birth after the age of 23 doubled the odds of premenopausal breast cancer. Reproductive factors (menarche and menopause) and lifestyle (al-cohol intake, smoking, contraceptive use, and breastfeeding duration) were not associated with premenopausal breast cancer in this cohort. About 13% of participants reported a family history of breast cancer, and 79% had prior knowledge of the disease. Conclusion: This study supports pre-vious reports of relatively higher incidence of aggressive disease in young Ghanaian women and the protective effect of early age at first birth. It further underscores the need to investigate its genetic underpinnings, whilst highlighting the importance of public education on self-examination tech-niques to reduce advanced disease presentation in Ghanaian women.