Xiaoxue Li

BACKGROUND: Vascular calcification (VC) is associated with diabetes, chronic kidney disease, and aging. VC is found to be a powerful and independent risk factor for cardiovascular mortality. Vascular smooth muscle cell (VSMC) ferroptosis, a form of cell death, is known to be involved in VC. However, whether VSMC ferroptosis is regulated by posttranslational modifications remains undefined. METHODS: We explored the potential role and mechanism of PARP1 (poly[ADP-ribose] polymerase 1)-mediated poly(ADP-ribosyl)ation (PARylation) in VSMC ferroptosis during VC. Mouse VSMCs were treated with β-glycerophosphate, and Parp1 ^flox/flox Tagln Cre ⁺ calcified mice were generated with AAV9-sh-POLG (DNA polymerase gamma) injected to establish in vitro and in vivo models, respectively. RNA-sequencing analysis was performed to determine the transcriptomic alterations in VSMCs overexpressing POLG and treated with β-glycerophosphate. RESULTS: Both PARP1 expression and PARylation levels were increased in β-glycerophosphate–induced VC, with PARP1 knockdown mitigating VC by improving mitochondrial function and inhibiting the subsequent VSMC ferroptosis. Mechanistically, POLG PARylation levels were increased in calcified VSMCs from PARP1 activation, triggering PARylation-dependent ubiquitination of POLG that resulted in POLG downregulation. This led to mitochondrial dysfunction and Adora2a (adenosine receptor A2A)/Rap1 (Ras-associated protein 1) signaling pathway activation to induce VSMC ferroptosis, which ultimately aggravated VC. CONCLUSIONS: Our study establishes the critical role of PARP1-mediated PARylation-dependent ubiquitination of POLG in VSMC ferroptosis-induced VC. These findings suggest that PARP1 inhibitors could potentially serve as novel therapeutic strategies for VC.

Oxidative cracking of alkenes is a significant process in industry. In this work, it was found that catalyzed by Se/Fe via hybrid mechanisms, the carbon-carbon double bond in alkenes can break to produce carbonyls under mild conditions. Since O₂ can be used as a partial oxidant, the employed H₂O₂ amount can be reduced (90 mol % vs 250 mol %) to avoid the peroxide residues, making the process even safer for operation.

The industrial Internet is a new industrial ecology, key infrastructure and new application mode that deeply integrates the new generation of information and communication technology with the industrial economy. It is based on the network, platform as the hub, data as the element, security as the guarantee, through the comprehensive connection of human, machine and material, it changes the traditional manufacturing mode, production organization mode and industrial form. The construction of a new industrial manufacturing and service system connecting the whole factors, the whole industrial chain and the whole value chain is the key and difficult point of enterprise management for supporting the construction of a strong manufacturing country and a strong network country, upgrading the modernization level of the industrial chain, promoting high-quality economic development, and building a new development pattern. At present, there are some problems in equipment management of domestic petrochemical enterprises, such as low efficiency of comprehensive management, insufficient integration of information system, difficulty in data sharing, inadequate integration of risk management technology, high maintenance cost, and great loss of shutdown. In recent years, the petrochemical industry equipment caused by the frequent production safety accidents, equipment management concept from institutionalized management to systematic management, digital, networking, intelligent importance of increasingly prominent. Statistics from the National Industrial Information Security Development and Research Center for the digital transformation of industrial enterprises show that equipment management and service demand account for the highest proportion. It is an inevitable choice to build a comprehensive and integrated equipment integrity management platform.

In this paper, an inverse synthetic aperture radar imaging method based on multiple norm constrained generalized orthogonal matching pursuit (MNGOMP) algorithm is proposed to improve the imaging process of inverse synthetic aperture radar is prone to noise interference in compressed sensing environment. Orthogonal matching pursuit (OMP) and generalized orthogonal matching pursuit (GOMP) converge slowly in radar imaging and are susceptible to noise interference. Therefore, multiple norm constraints were introduced into the iteration update to improve convergence speed and accuracy and enhance noise resistance. The results show that this method is effective in suppressing unnecessary clutter and enhancing desired target.

2,5-Bis(p-benzoquinonyl) pyridine (QPQ-2) was developed as the cathode for sodium-ion batteries.