Hyo Jung Lim
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Research Interests
Hyo Jung Lim has a total of 5 co-authors. Here are the top co-authors by number of shared publications:
- Dong-Hyun Jung
Division of Food and Nutrition, Chonnam National University, Gwangju, Republic of Korea
1 shared publication - Inonge Noni Siziya
Division of Food and Nutrition, Chonnam National University, Gwangju, Republic of Korea
1 shared publication - Myung-Ji Seo
Department of Bioengineering and Nano-Bioengineering, Incheon National University, Incheon, Republic of Korea
1 shared publication - Sang Gil Lee
Department of Food Science and Nutrition, Pukyong National University, Busan, Republic of Korea
1 shared publication - Suhyeon Baek
Department of Smart Green Technology Engineering, Pukyong National University, Busan 48513, Republic of Korea
1 shared publication
Latest Publications
- 15 February 2025
- Published by Springer Nature in Food Science and Biotechnology
- Vol. 34 (10) , 2225-2235
Enhancement of iminosugar production, 1-deoxynojirimycin and 1-deoxymannojirimycin, in recombinant Corynebacterium glutamicum
The iminosugars 1-deoxynojirimycin (1-DNJ) and 1-deoxymannojirimycin (1-DMJ) were produced by recombinant Corynebacterium glutamicum (CgTYB) bearing the 1-DNJ-producing GabT1-Yktc1-GutB1 (TYB) gene cluster from Bacillus velezensis MBLB0692. The enhanced iminosugar biosynthesis in CgTYB cultures increased both α-mannosidase inhibition (AMI) and α-glucosidase inhibition (AGI) activity. Individual cultures harboring GabT1, Yktc1, and GutB1 genes were found to be able to produce both 1-DNJ and 1-DMJ in mixed cultures of one pot analysis. This suggests that the genes necessary for epimerization and reduction are intrinsic to the cells rather than close to the 1-DNJ-producing cluster. Glucose was the preferred carbon source and a provision of 10 g/L glucose increased AGI and AMI to 69.8% and 70.1%, respectively. The 1 mM IPTG produced 55.0% AGI and 72.1% AMI, and 20% ethanolic permeabilization produced 62.8% AGI and 74.2% AMI. Batch fermentation increased iminosugar yields from 18.7 to 229.9 mg/L 1-DNJ, and 41.8 to 63.7 mg/mL 1-DMJ.
- 15 February 2025
- Published by Springer Nature in Food Science and Biotechnology
- Vol. 34 (10) , 2225-2235
Enhancement of iminosugar production, 1-deoxynojirimycin and 1-deoxymannojirimycin, in recombinant Corynebacterium glutamicum
The iminosugars 1-deoxynojirimycin (1-DNJ) and 1-deoxymannojirimycin (1-DMJ) were produced by recombinant Corynebacterium glutamicum (CgTYB) bearing the 1-DNJ-producing GabT1-Yktc1-GutB1 (TYB) gene cluster from Bacillus velezensis MBLB0692. The enhanced iminosugar biosynthesis in CgTYB cultures increased both α-mannosidase inhibition (AMI) and α-glucosidase inhibition (AGI) activity. Individual cultures harboring GabT1, Yktc1, and GutB1 genes were found to be able to produce both 1-DNJ and 1-DMJ in mixed cultures of one pot analysis. This suggests that the genes necessary for epimerization and reduction are intrinsic to the cells rather than close to the 1-DNJ-producing cluster. Glucose was the preferred carbon source and a provision of 10 g/L glucose increased AGI and AMI to 69.8% and 70.1%, respectively. The 1 mM IPTG produced 55.0% AGI and 72.1% AMI, and 20% ethanolic permeabilization produced 62.8% AGI and 74.2% AMI. Batch fermentation increased iminosugar yields from 18.7 to 229.9 mg/L 1-DNJ, and 41.8 to 63.7 mg/mL 1-DMJ.