Marisol Rosas-Diaz
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Research Interests
Marisol Rosas-Diaz has a total of 15 co-authors. Here are the top co-authors by number of shared publications:
- Ana P. Juarez-Islas
Molecular Biology Laboratory of Hospital Clinic Huauchinango, Institute of Social Security and Services for State Workers, Huauchinango 73160, Puebla, Mexico
1 shared publication - Carmen Palacios-Reyes
Department of Medical Sciences, Division of Health Sciences, University of Guanajuato, Campus Leon, León de los Aldama 37000, Guanajuato, Mexico
1 shared publication - Deyanira Escalante-Bautista
UIM en Genética Humana, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Ciudad de México 06600, Mexico
1 shared publication - Esperanza Milagros Garcia Oropesa
Autonomous University of Tamaulipas, Reynosa, Tamaulipas, México
1 shared publication - Gabriela Salas-Cuevas
Molecular Biology Laboratory of Hospital Clinic Huauchinango, Institute of Social Security and Services for State Workers, Huauchinango 73160, Puebla, Mexico
1 shared publication - Griselda León Burgoa
Public Health Laboratory of Puebla, Secretary of Health Mexico, Puebla 772490, Mexico
1 shared publication
Latest Publications
- Open Access
- 23 July 2025
- Published by MDPI AG in Current Issues in Molecular Biology
- Vol. 47 (8) , 585
New Tool Against Tuberculosis: The Potential of the LAMP Lateral Flow Assay in Resource-Limited Settings
Tuberculosis (TB) is a global public health issue requiring early and accurate diagnosis. The loop-mediated isothermal amplification (LAMP) assay is a promising alternative recommended by the WHO for the initial diagnosis of pulmonary TB, particularly in resource-limited settings. This study evaluated the sensitivity and specificity of a commercial LAMP assay for TB detection using 198 samples from different countries including Mexico. The LAMP assay results were compared to the results of standard tests: AFB smear microscopy, cell culture, and Xpert PCR. Across all samples, LAMP showed a sensitivity of 96.20% and a specificity of 84.61%. When compared specifically to “true positives” and “true negatives” (defined by the consistency across the standard tests), LAMP demonstrated 100% sensitivity and 92.30% specificity. For context, the sensitivity of AFB smear microscopy against the culture and Xpert tests was 79.04%. A significant finding was that the LAMP test detected a high percentage (92.5%) of samples found positive by the culture and Xpert tests but negative by the AFB smear, highlighting its ability to identify cases missed by traditional microscopy. This study concluded that the LAMP assay is a sensitive and specific tool for TB diagnosis with potential for rapid and accurate diagnosis, especially in resource-limited areas.
- 23 July 2025
- Published by MDPI AG in Current Issues in Molecular Biology
- Vol. 47 (8) , 585
Open AccessNew Tool Against Tuberculosis: The Potential of the LAMP Lateral Flow Assay in Resource-Limited Settings
Tuberculosis (TB) is a global public health issue requiring early and accurate diagnosis. The loop-mediated isothermal amplification (LAMP) assay is a promising alternative recommended by the WHO for the initial diagnosis of pulmonary TB, particularly in resource-limited settings. This study evaluated the sensitivity and specificity of a commercial LAMP assay for TB detection using 198 samples from different countries including Mexico. The LAMP assay results were compared to the results of standard tests: AFB smear microscopy, cell culture, and Xpert PCR. Across all samples, LAMP showed a sensitivity of 96.20% and a specificity of 84.61%. When compared specifically to “true positives” and “true negatives” (defined by the consistency across the standard tests), LAMP demonstrated 100% sensitivity and 92.30% specificity. For context, the sensitivity of AFB smear microscopy against the culture and Xpert tests was 79.04%. A significant finding was that the LAMP test detected a high percentage (92.5%) of samples found positive by the culture and Xpert tests but negative by the AFB smear, highlighting its ability to identify cases missed by traditional microscopy. This study concluded that the LAMP assay is a sensitive and specific tool for TB diagnosis with potential for rapid and accurate diagnosis, especially in resource-limited areas.