Wenfeng Guo

Accurate leaf area measurement is essential for plant growth monitoring and ecological research; however, it is often challenged by perspective distortion and color inconsistencies resulting from variations in shooting conditions and plant status. To address these issues, this study proposes a visual and semi-automatic measurement system. The system utilizes Hough transform-based perspective transformation to correct perspective distortions and incorporates manually sampled points to obtain prior color information, effectively mitigating color inconsistency. Based on this prior knowledge, the level-set function is automatically initialized. The leaf extraction is achieved through level-set curve evolution that minimizes an energy function derived from a multivariate Gaussian distribution model, and the evolution process allows visual monitoring of the leaf extraction progress. Experimental results demonstrate robust performance under diverse conditions: the standard deviation remains below 1 cm², the relative error is under 1%, the coefficient of variation is less than 3%, and processing time is under 10 s for most images. Compared to the traditional labor-intensive and time-consuming manual photocopy-weighing approach, as well as OpenPheno (which lacks parameter adjustability) and ImageJ 1.54g (whose results are highly operator-dependent), the proposed system provides a more flexible, controllable, and robust semi-automatic solution. It significantly reduces operational barriers while enhancing measurement stability, demonstrating considerable practical application value.