Rhys T. Meredith
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Research Interests
Rhys T. Meredith has a total of 6 co-authors. Here are the top co-authors by number of shared publications:
- Hayley Mills
InBio, Cardiff CF23 8HA, UK
1 shared publication - James Blaxland
Cardiff School of Sport and Health Sciences, Cardiff Metropolitan University, Cardiff CF5 2YB, UK
1 shared publication - Maria Àngels Oliver
InBio, Cardiff, United Kingdom
1 shared publication - Martin D. Chapman
InBio Charlottesville Virginia USA
1 shared publication - Max D. D Bermingham
INBIO
1 shared publication - Sarah E. Maddocks
Microbiology & Infection Research Group, Cardiff School of Sport and Health Sciences, Cardiff Metropolitan University, Cardiff CF5 2YB, UK
1 shared publication
Latest Publications
- Open Access
Optimising Extraction of Specific Food Allergens from Challenging Food Matrices for Immunoassay Quantification
Simultaneous extraction of multiple clinically relevant, specific allergens from complex, processed foods for immunoassay quantification remains challenging. Moreover, shared extraction buffers remain elusive, which limits the effectiveness of multiplex (multi-target) technology. This study aimed to optimise extraction of specific allergens from challenging food samples and identify shared methods of extraction for multiplex analysis. Incurred processed food matrices (chocolate dessert, raw and baked biscuit) were prepared and extracted with 10 different buffers varying in base, pH, and additive content. Extracts were analysed by allergen-specific multiplex array and ELISA. Optimised recovery of 14 food allergens was obtained from complex incurred matrices using two extraction buffers: 50 mM carbonate bicarbonate with 10% fish gelatine, and PBS with 2%-Tween, 1 M NaCl with 10% fish gelatine and 1% PVP. In most cases, optimised buffers provided 50–150% recovery from incurred foods. Matrices that contained chocolate or were subject to thermal processing had lower recoveries. Optimised allergen extraction methods can be used to analyse foods associated with treatment and prevention of allergy and reference materials for clinically relevant allergen content and identify allergen contamination. Identification of shared, optimised extraction buffers will result in increased sample throughput in multiplex immunoassay techniques.
Open AccessOptimising Extraction of Specific Food Allergens from Challenging Food Matrices for Immunoassay Quantification
Simultaneous extraction of multiple clinically relevant, specific allergens from complex, processed foods for immunoassay quantification remains challenging. Moreover, shared extraction buffers remain elusive, which limits the effectiveness of multiplex (multi-target) technology. This study aimed to optimise extraction of specific allergens from challenging food samples and identify shared methods of extraction for multiplex analysis. Incurred processed food matrices (chocolate dessert, raw and baked biscuit) were prepared and extracted with 10 different buffers varying in base, pH, and additive content. Extracts were analysed by allergen-specific multiplex array and ELISA. Optimised recovery of 14 food allergens was obtained from complex incurred matrices using two extraction buffers: 50 mM carbonate bicarbonate with 10% fish gelatine, and PBS with 2%-Tween, 1 M NaCl with 10% fish gelatine and 1% PVP. In most cases, optimised buffers provided 50–150% recovery from incurred foods. Matrices that contained chocolate or were subject to thermal processing had lower recoveries. Optimised allergen extraction methods can be used to analyse foods associated with treatment and prevention of allergy and reference materials for clinically relevant allergen content and identify allergen contamination. Identification of shared, optimised extraction buffers will result in increased sample throughput in multiplex immunoassay techniques.