Congguo Ran

Two bacterial strains, designated BSP-53 ^T and BSP-153 ^T , were isolated from soil samples collected from Beijing Olympic Forest Park, China. Phylogenomic reconstruction based on core genes robustly positioned the strains as two distinct evolutionary lineages within the genus Acinetobacter . Both strains were Gram-negative, aerobic, non-motile and short rods. Their major cellular fatty acids consisted of summed feature 3 (C _16 : 1  ω 7 c /C _16 : 1  ω 6 c ), C _18 : 1  ω 9 c , C _16 : 0 , C _12 : 0 and C _12 : 0 3-OH. The predominant polar lipids included diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The average nucleotide identity values between the novel strains and their closest Acinetobacter type strains were below 95%, with corresponding digital DNA–DNA hybridization values below 70%. Based on polyphasic taxonomic characterization, including chemotaxonomic, phenotypic, phylogenetic and genomic analysis, we propose that strains BSP-53 ^T and BSP-153 ^T represent two novel species of the genus Acinetobacter , for which the names Acinetobacter uliginosus sp. nov. and Acinetobacter halobius sp. nov. are designated. The type strains are BSP-53 ^T (=CGMCC 1.62070 ^T =KCTC 8190 ^T ) and BSP-153 ^T (=CGMCC 1.62071 ^T =KCTC 8191 ^T ), respectively.

A newly discovered facultative anaerobic strain, designated as LXY-3^T, was obtained from a soil sample collected at an industrial site in Guangxi, China, known for heavy metal processing. An investigation including phenotypic, chemotaxonomic, and genomic traits was conducted. Phylogenetic analysis based on 16S rRNA showed that LXY-3^T belonged to the genus Paenibacillus. The closest phylogenetic relative of this strain was Paenibacillus anaericanus MH21^T with the similarity of 97.03%. Iso-C_15:0, antéiso-C_15:0, and C_16:1 ω7c alcohol were the major cellular fatty acids. The predominant polar lipids comprised diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), unidentified phospholipids (PL1-PL8), unidentified resistant material (RM1–RM4), and lipids (L1–L3). For genome sequencing, the genomic DNA G+C content of the strain is 51.2 mol%. Comparative genomic analysis revealed that the average nucleotide identity (ANI) values between strain LXY-3^T and its closest phylogenetic relatives within the genus Paenibacillus (represented by type strains) were consistently below the 95% species demarcation threshold. Nitrogen fixation gene cluster (nifB, nifE, nifK, nifN, nifV, nifX, nifD, and nifH) was conserved in the strain. Correspondingly, digital DNA–DNA hybridization (dDDH) values remained below the 70% cutoff for species delineation. These genomic metrics provide compelling evidence that strain LXY-3^T represents a novel species within the genus Paenibacillus. The type strain LXY-3^T (=CGMCC 1.64949^T = JCM 37600^T) is proposed.

Two bacterial strains, designated LXY-1^T and LXY-4^T, were isolated from soil samples collected at a heavy metal smelting plant located in Guangxi, China. Phylogenetic analysis indicated that these strains formed two distinct lineages within the genus Neobacillus. Both strains were characterized as facultative anaerobic, Gram-positive staining, endospore-forming, non-motile, short-rod bacteria. The major cellular fatty acids identified in these strains included C_16:0, iso-C_15:0, antéiso-C_15:0, and antéiso-C_17:0. The predominant polar lipids comprised diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), and phosphatidylglycerol (PG). The average nucleotide identity (ANI) values between the newly isolated strains and their closest phylogenetic relatives, the type strains of the genus Neobacillus, were found to be below 95%, with corresponding digital DNA–DNA hybridization (dDDH) values remaining below 70%. Based on a comprehensive polyphasic taxonomic analysis incorporating chemotaxonomic, phenotypic, phylogenetic, and genomic data, we proposed that strains LXY-1^T and LXY-4^T represent two novel species of the genus Neobacillus, for which the names Neobacillus terrisolis sp. nov. and Neobacillus solisequens sp. nov. are designated. The type strains are LXY-1^T (= CGMCC 30313^T = JCM 37671^T) and LXY-4^T (= CGMCC 1.62901^T = JCM 37672^T), respectively.