Marta Guerrero
Research Subjects
Marta Guerrero has a total of 8 co-authors. Here are the top co-authors by number of shared publications:
- Daniel Narvaez
Retinset S.L., Amposta 20, 08174 Sant Cugat del Vallès, Spain
1 shared publication - Enzo D’ambrosio
Centro Oftalmico d’Ambrosio, 74121 Taranto, Italy
1 shared publication - Francesco D'ORia
Section of Ophthalmology, Department of Translational Biomedicine and Neuroscience, University of Bari, Bari, Italy
1 shared publication - Francesco Pignatelli
Eye Clinic, “SS. Annunziata” Hospital, ASL Taranto, 74100 Taranto, Italy
1 shared publication - Giovanni Alessio
Department of Translational Biomedicine Neuroscience, University of Bari “Aldo Moro”, 70124 Bari, Italy
1 shared publication - Giovanni Petruzzella
Section of Ophthalmology, Department of Translational Biomedicine and Neuroscience, University of Bari, 70121 Bari, Italy
1 shared publication
Latest Publications
- Open Access
Inhibitory Effect of a Novel Ophthalmic Solution on Acanthamoeba castellanii Adhesion and Biofilm Formation on Human Corneal Epithelium
Background/Objectives: Acanthamoeba keratitis (AK) is a rare but sight-threatening corneal infection, often associated with contact lens wear and resistant to conventional therapies. Preventive strategies capable of reducing Acanthamoeba adhesion to corneal epithelium may represent an important tool for infection control. This study aimed to evaluate the amebicidal and preventive activity of CORNEIAL MED eye drops against Acanthamoeba castellanii adhesion and early adhesion layer on human corneal epithelium (HCE). Methods: Reconstructed HCE models were exposed to A. castellanii under four experimental conditions: negative control (HCE only), positive control (HCE + A. castellanii), co-incubation with CORNEIAL MED and A. castellanii (Study 1), and treatment with CORNEIAL MED after initial A. castellanii adhesion (Study 2). Adherent amoebae were quantified using EDTA detachment and Neubauer chamber counting. The early adhesion layer was characterized by scanning electron microscopy (SEM). Statistical analysis considered p < 0.05 as significant. Results: In Study 1, simultaneous application of CORNEIAL MED with A. castellanii reduced amoeba adhesion by 33.0 ± 11% compared with controls (p = 0.0529). In Study 2, when the product was applied 3 h after amoeba inoculation, adhesion was significantly reduced by 51.9 ± 6.5% (p < 0.05). SEM confirmed a decrease in amoebic colonization and biofilm density in treated samples. Conclusions: CORNEIAL MED demonstrated a measurable inhibitory effect on A. castellanii adhesion to HCE, particularly when applied after initial pathogen contact. These findings suggest a potential preventive role of CORNEIAL MED in reducing AK risk, although further in vivo studies are warranted.
Open AccessInhibitory Effect of a Novel Ophthalmic Solution on Acanthamoeba castellanii Adhesion and Biofilm Formation on Human Corneal Epithelium
Background/Objectives: Acanthamoeba keratitis (AK) is a rare but sight-threatening corneal infection, often associated with contact lens wear and resistant to conventional therapies. Preventive strategies capable of reducing Acanthamoeba adhesion to corneal epithelium may represent an important tool for infection control. This study aimed to evaluate the amebicidal and preventive activity of CORNEIAL MED eye drops against Acanthamoeba castellanii adhesion and early adhesion layer on human corneal epithelium (HCE). Methods: Reconstructed HCE models were exposed to A. castellanii under four experimental conditions: negative control (HCE only), positive control (HCE + A. castellanii), co-incubation with CORNEIAL MED and A. castellanii (Study 1), and treatment with CORNEIAL MED after initial A. castellanii adhesion (Study 2). Adherent amoebae were quantified using EDTA detachment and Neubauer chamber counting. The early adhesion layer was characterized by scanning electron microscopy (SEM). Statistical analysis considered p < 0.05 as significant. Results: In Study 1, simultaneous application of CORNEIAL MED with A. castellanii reduced amoeba adhesion by 33.0 ± 11% compared with controls (p = 0.0529). In Study 2, when the product was applied 3 h after amoeba inoculation, adhesion was significantly reduced by 51.9 ± 6.5% (p < 0.05). SEM confirmed a decrease in amoebic colonization and biofilm density in treated samples. Conclusions: CORNEIAL MED demonstrated a measurable inhibitory effect on A. castellanii adhesion to HCE, particularly when applied after initial pathogen contact. These findings suggest a potential preventive role of CORNEIAL MED in reducing AK risk, although further in vivo studies are warranted.