Jie Huang
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Jie Huang has a total of 5 co-authors. Here are the top co-authors by number of shared publications:
- Haoyu Wang
College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China
1 shared publication - Heng Sun
College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China
1 shared publication - Yao Wu
GeneVide Biotech Co., Ltd., Suzhou 210500, China
1 shared publication - Yucong Huang
Fisheries College of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Zhanjiang, China
1 shared publication - Zhenxin Hu
Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Fisheries College of Guangdong Ocean University, Zhanjiang 524088, China
1 shared publication
Latest Publications
- Open Access
Integrated Enzyme-Mediated One-Step Sample Processing and Duplex Amplification System for Rapid Detection of Carpione rhabdovirus in Aquaculture-Derived Food Products
Golden pompano (Trachinotus ovatus) is the largest-scale marine aquaculture fish species in China, with a significant economic and nutritional value as a high-quality seafood product. The recent outbreak of an epidemic caused by a novel Carpione rhabdovirus (CAPRV) occurred in cultured golden pompano. To address it, a CAPRV enzyme-mediated one-step sample processing–reverse transcription–enzyme-mediated duplex exponential amplification (EmOSP-RT-EmDEA) detection system was developed. This innovative molecular diagnostic tool integrates enzyme-mediated one-step sample processing (EmOSP) with enzyme-mediated duplex exponential amplification (EmDEA) technology. Unlike traditional RPA-Cas12a detection methods, this system directly incorporates fluorophores into RNA components, eliminating the need for exogenous fluorescent probes while maintaining high sensitivity. It enables rapid, sensitive, and specific detection of CAPRV2023 across various sample types, including clinical, invasive, minimally invasive, and environmental specimens. Performance evaluation of the CAPRV2023 EmOSP-RT-EmDEA detection system against conventional diagnostic methods, such as TaqMan qPCR and traditional PCR, demonstrated superior sensitivity, with a detection limit as low as 4 copies/μL, and exceptional specificity. The optimized EmOSP protocol for nucleic acid extraction from fecal, hepatic, and water samples provided robust and reproducible results. The EmOSP-RT-EmDEA system achieved a detection rate of 68.14% in fecal samples, matching the performance of the gold-standard TaqMan qPCR assay.
Open AccessIntegrated Enzyme-Mediated One-Step Sample Processing and Duplex Amplification System for Rapid Detection of Carpione rhabdovirus in Aquaculture-Derived Food Products
Golden pompano (Trachinotus ovatus) is the largest-scale marine aquaculture fish species in China, with a significant economic and nutritional value as a high-quality seafood product. The recent outbreak of an epidemic caused by a novel Carpione rhabdovirus (CAPRV) occurred in cultured golden pompano. To address it, a CAPRV enzyme-mediated one-step sample processing–reverse transcription–enzyme-mediated duplex exponential amplification (EmOSP-RT-EmDEA) detection system was developed. This innovative molecular diagnostic tool integrates enzyme-mediated one-step sample processing (EmOSP) with enzyme-mediated duplex exponential amplification (EmDEA) technology. Unlike traditional RPA-Cas12a detection methods, this system directly incorporates fluorophores into RNA components, eliminating the need for exogenous fluorescent probes while maintaining high sensitivity. It enables rapid, sensitive, and specific detection of CAPRV2023 across various sample types, including clinical, invasive, minimally invasive, and environmental specimens. Performance evaluation of the CAPRV2023 EmOSP-RT-EmDEA detection system against conventional diagnostic methods, such as TaqMan qPCR and traditional PCR, demonstrated superior sensitivity, with a detection limit as low as 4 copies/μL, and exceptional specificity. The optimized EmOSP protocol for nucleic acid extraction from fecal, hepatic, and water samples provided robust and reproducible results. The EmOSP-RT-EmDEA system achieved a detection rate of 68.14% in fecal samples, matching the performance of the gold-standard TaqMan qPCR assay.