Naizhong Hu

This study aimed to elucidate the genetic variation patterns of hybrid larch during generational transitions, providing a theoretical basis for targeted genetic improvement and advanced hybrid breeding of pulpwood. Seven hybrid larch families and their progeny from the Qingshan National Larch Elite Seed Base in Linkou County, Heilongjiang Province, were used as experimental materials. Growth traits, wood properties, and pulping performance of both generations were measured and analyzed, and pulp production capacity was calculated accordingly. Significant differences were observed between the F₁ and F₂ generations across all traits. The F₂ generation showed greater genetic stability, though it was more susceptible to environmental factors. The LK3 × LG2 family was identified as elite through principal component analysis (PCA) and membership function analysis. Further analysis of intergenerational data confirmed that the LK5 × LO78-3 family exhibited superior genetic stability across generations, making it an optimal parental source. This study establishes a genetic foundation for the targeted genetic improvement of larch pulpwood, with important implications for advanced hybrid breeding and elite family selection.

Pinus koraiensis, as a keystone tree species, possesses immense economic and ecological value. However, the present cultivation of high-quality seedlings in Pinus koraiensis plantations remains hindered by prohibitively high costs and inadequate technological advancements. Additionally, the species’ prolonged growth cycle and low yield, when compounded by issues such as excessive harvesting, may result in supply constraints. Plant growth regulators (PGRs), a class of naturally occurring or synthetically derived chemical compounds, are capable of modulating plant development and physiology. These regulators exert notable effects by enhancing root proliferation, facilitating lignification, influencing plant architecture, and augmenting yield. Owing to their operational simplicity and relatively low cost, PGR applications hold substantial promise for cultivating Pinus koraiensis seedlings with superior traits. In this study, four-year-old Pinus koraiensis seedlings were employed to evaluate the impacts of three PGRs (paclobutrazol, chlormequat chloride, and diethyl aminoethyl hexanoate), alongside varied application methods (dosage and frequency), on the growth, physiological, and photosynthetic parameters of the seedlings. The findings revealed that treatment with 1.5 g/L paclobutrazol produced the most pronounced effects across a range of indicators. Specifically, this treatment markedly enhanced growth traits (e.g., branch diameter, new shoot length, lateral branch length, aboveground fresh and dry weights, root fresh and dry weights, lateral root dry weight, and number of second-order roots), physiological attributes (e.g., increased superoxide dismutase and peroxidase activities, elevated lignin content, and reduced relative conductivity and malondialdehyde levels), and photosynthetic metrics (e.g., elevated net photosynthetic rate, stomatal conductance, transpiration rate, and maximum net photosynthetic rate), thereby constituting the optimal treatment combination.