Caroline M. Palmentiero
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Research Interests
Caroline M. Palmentiero has a total of 12 co-authors. Here are the top co-authors by number of shared publications:
- Colm P. Roster
Eukaryotic Pathogens Innovation Center, Department of Genetics and Biochemistry, Clemson University, Clemson, SC 29634, USA
3 shared publications - James C. Morris
Eukaryotic Pathogens Innovation Center, Department of Genetics and Biochemistry, Clemson University, Clemson, SC 29634, USA
3 shared publications - Jillian Elisa Milanes McKeon
Clemson University
3 shared publications - Daniel Charles Whitehead
Department of Chemistry, Clemson University, Clemson, SC 29634, USA
1 shared publication - Jennifer E. Golden
Department of Chemistry, University of Wisconsin-Madison, 1101 University Ave, Madison, WI, 53706, United States of America; Division of Pharmaceutical Sciences, University of Wisconsin-Madison, 777 Highland Ave., Madison, WI, 537055, United States of America
1 shared publication - Khairul I. Khan
Department of Chemistry, University of Louisville, Louisville, KY, United States of America
1 shared publication
Latest Publications
- Open Access
- 7 July 2026
- Published by Elsevier in International Journal for Parasitology: Drugs and Drug Resistance
- Vol. 31, 100656
Characterization of two early lead anti-amoebic agents with activity against Naegleria fowleri
- 7 July 2026
- Published by Elsevier in International Journal for Parasitology: Drugs and Drug Resistance
- Vol. 31, 100656
Open AccessCharacterization of two early lead anti-amoebic agents with activity against Naegleria fowleri
- Open Access
Development of Approaches for Transgene Expression in the Pathogenic Free-Living Amoeba Naegleria fowleri
The absence of molecular tools for manipulation of gene expression in the pathogenic free-living amoeba Naegleria fowleri has historically limited our understanding of gene function in the organism and has coincidently impacted the identification of potential druggable pathways and proteins. Here, we describe the development of approaches for the generation of transgenic amoebae using polyethyleneimine nanoparticles to deliver plasmids designed to confer antibiotic resistance and fluorescence to the cells. Through a series of optimization steps, we found that transfection of plasmids encoding the fluorescent protein mCherry fused by a T2A self-cleaving peptide to a codon-optimized puromycin acetyltransferase selectable marker yielded fluorescent cells that were resistant up to 100 µg/mL puromycin. Transfected trophozoites harbored between 45 and 65 copies of the transgene per cell and both fluorescence and resistance were persistent in the presence of selector through continued passages. The development of these approaches is anticipated to enable application of an array of genetic manipulation techniques including forward and reverse genetics to the study of this important pathogen.
Open AccessDevelopment of Approaches for Transgene Expression in the Pathogenic Free-Living Amoeba Naegleria fowleri
The absence of molecular tools for manipulation of gene expression in the pathogenic free-living amoeba Naegleria fowleri has historically limited our understanding of gene function in the organism and has coincidently impacted the identification of potential druggable pathways and proteins. Here, we describe the development of approaches for the generation of transgenic amoebae using polyethyleneimine nanoparticles to deliver plasmids designed to confer antibiotic resistance and fluorescence to the cells. Through a series of optimization steps, we found that transfection of plasmids encoding the fluorescent protein mCherry fused by a T2A self-cleaving peptide to a codon-optimized puromycin acetyltransferase selectable marker yielded fluorescent cells that were resistant up to 100 µg/mL puromycin. Transfected trophozoites harbored between 45 and 65 copies of the transgene per cell and both fluorescence and resistance were persistent in the presence of selector through continued passages. The development of these approaches is anticipated to enable application of an array of genetic manipulation techniques including forward and reverse genetics to the study of this important pathogen.
- Open Access
- 10 December 2025
- Published by MDPI AG in Preprints.org
Development of Approaches for Transgene Expression in the Pathogenic Free-Living Amoeba Naegleria fowleri
The absence of molecular tools for manipulation of gene expression in the pathogenic free-living amoeba Naegleria fowleri has historically limited our understanding of gene function in the organism and has coincidently impacted the identification of potential druggable pathways and proteins. Here, we describe the development of approaches for the generation of transgenic amoebae using polyethyleneimine nanoparticles to deliver plasmids designed to confer antibiotic resistance and fluorescence to the cells. Through a series of optimization steps, we found that transfection of plasmids encoding the fluorescent protein mCherry fused by a T2A self-cleaving peptide to a codon-optimized puromycin acetyltransferase selectable marker yielded fluorescent cells that were resistant up to 100 µg/mL puromycin. Transfected trophozoites harbored between 45 and 65 copies of the transgene per cell and both fluorescence and resistance were persistent in the presence of selector through continued passages. The development of these approaches is anticipated to enable application of an array of genetic manipulation techniques including forward and reverse genetics to the study of this important pathogen.
- 10 December 2025
- Published by MDPI AG in Preprints.org
Open AccessDevelopment of Approaches for Transgene Expression in the Pathogenic Free-Living Amoeba Naegleria fowleri
The absence of molecular tools for manipulation of gene expression in the pathogenic free-living amoeba Naegleria fowleri has historically limited our understanding of gene function in the organism and has coincidently impacted the identification of potential druggable pathways and proteins. Here, we describe the development of approaches for the generation of transgenic amoebae using polyethyleneimine nanoparticles to deliver plasmids designed to confer antibiotic resistance and fluorescence to the cells. Through a series of optimization steps, we found that transfection of plasmids encoding the fluorescent protein mCherry fused by a T2A self-cleaving peptide to a codon-optimized puromycin acetyltransferase selectable marker yielded fluorescent cells that were resistant up to 100 µg/mL puromycin. Transfected trophozoites harbored between 45 and 65 copies of the transgene per cell and both fluorescence and resistance were persistent in the presence of selector through continued passages. The development of these approaches is anticipated to enable application of an array of genetic manipulation techniques including forward and reverse genetics to the study of this important pathogen.