Caspian Harding
Research Subjects
Caspian Harding has a total of 10 co-authors. Here are the top co-authors by number of shared publications:
- Andrew C. Nielsen
Syntax Bio Inc., Chicago, IL 60642, USA
1 shared publication - Anupama K. Puppala
Syntax Bio Inc., Chicago, IL 60642, USA
1 shared publication - Bradley James Merrill
Department of Biochemistry and Molecular Genetics, University of Illinois at Chicago, Chicago, IL, USA
1 shared publication - Georgina Mancinelli
Syntax Bio Inc., Chicago, IL 60642, USA
1 shared publication Maureen Regan
Syntax Bio Inc., Chicago, IL 60642, USA
1 shared publication- Michaele McGregor
Syntax Bio Inc., Chicago, IL 60642, USA
1 shared publication
Latest Publications
- Open Access
- 5 December 2025
- Published by American Association for the Advancement of Science (AAAS) in Science Advances
- Vol. 11 (49) , eadt1532
Programmable multistep CRISPR gene activation via control of RNA polymerase III termination
Although genomes encode instructions for mammalian cell differentiation with rich syntactic relationships, existing methods for genetically programming cells have only modest capabilities for stepwise gene regulation. Here, we develop a sequential genetic system that transcriptionally activates endogenous genes in a preprogrammed, stepwise manner. This system uses the removal of an RNA polymerase III termination sequence to trigger both the transcriptional activation and DNA endonuclease activities of a Cas9-VPR protein, driving progression through a cascade of gene activation events. The system’s functionality in human cells, including iPSCs, enables the development of a path for cellular programming by controlling the sequential order of gene activation to influence cellular states.
- 5 December 2025
- Published by American Association for the Advancement of Science (AAAS) in Science Advances
- Vol. 11 (49) , eadt1532
Open AccessProgrammable multistep CRISPR gene activation via control of RNA polymerase III termination
Although genomes encode instructions for mammalian cell differentiation with rich syntactic relationships, existing methods for genetically programming cells have only modest capabilities for stepwise gene regulation. Here, we develop a sequential genetic system that transcriptionally activates endogenous genes in a preprogrammed, stepwise manner. This system uses the removal of an RNA polymerase III termination sequence to trigger both the transcriptional activation and DNA endonuclease activities of a Cas9-VPR protein, driving progression through a cascade of gene activation events. The system’s functionality in human cells, including iPSCs, enables the development of a path for cellular programming by controlling the sequential order of gene activation to influence cellular states.