Philipe Herman Nfombouot Njitoyap
Philipe Herman Nfombouot Njitoyap has a total of 11 co-authors. Here are the top co-authors by number of shared publications:
- Arnaud Fondjo Kouam
University of Buea Faculty of Health Sciences
2 shared publications - Elisabeth Zeuko’o Menkem
University of Buea Faculty of Health Sciences
2 shared publications - Frédéric Nico Njayou
Department of Biochemistry, Faculty of Science, University of Yaoundé 1, Yaoundé, Cameroon
2 shared publications - Paul Fewou Moundipa
Laboratory of Toxicology & Pharmacology, Department of Biochemistry, Faculty of Science, University of Yaounde 1, BP 812 Yaounde, Cameroon
2 shared publications - Armel Jackson Seukep
Pharmacology Laboratory, Department of Biomedical Sciences, Faculty of Health Sciences, University of Buea, PO Box 63, Buea, Cameroon
1 shared publication - Cromwel Tepap Zemnou
School of Engineering BiomedTech, EuroMed University of Fes (UEMF), Fes 30070, Morocco
1 shared publication
Latest Publications
- Open Access
- 19 February 2026
- Published by AccScience Publishing in Microbes & Immunity
- Vol. 3 (3) , 025470126
Evaluation of chicken egg yolk immunoglobulin Y-based ELISA for serodiagnosis of hepatitis E virus in swine
Hepatitis E virus (HEV) is a major zoonotic pathogen, and pigs are an important reservoir. Serology for anti-HEV antibodies poorly reflects active infection, while reverse transcription polymerase chain reaction (RT-PCR) is sensitive but not always feasible for large-scale surveillance. This study aimed to develop an immunoglobulin Y (IgY)-based sandwich enzyme-linked immunosorbent assay (ELISA) for direct detection of HEV antigen (HEV-Ag). An antigenic epitope from the HEV open reading frame 2 capsid protein was identified in silico, synthesized, and used to immunize laying hens; IgY was purified from egg yolk and characterized by sodium dodecyl sulfate–polyacrylamide gel electrophoresis. An in-house sandwich ELISA was subsequently developed using the anti-HEV IgY antibodies. Its diagnostic performance was evaluated against RT-PCR using 350 field-collected pig serum samples and compared with commercial anti-HEV IgG and IgM ELISA kits. Immunization yielded high-purity IgY antibodies, with antigen-specific antibody kinetics showing a significant increase in specific immunoreactivity from week 4 onward and peak sensitivity at weeks 6–8 post-immunization. The anti-HEV IgY showed high specificity, with no cross-reactivity observed against a heterologous antigen (HBsAg). The optimized sandwich ELISA detected HEV-Ag with a sensitivity of 95.4% and a specificity of 100% relative to RT-PCR. The prevalence of HEV-Ag was 17.7% (62/350), which was not significantly different from RT-PCR (18.6%, 65/350; p=0.250). In contrast, commercial IgG and IgM ELISAs showed high seroprevalence rates (34.9% and 28.3%, respectively) but exhibited significant discrepancies with RT-PCR (p<0.0001), indicating limited utility for identifying active infection. Overall, the developed IgY-based sandwich ELISA demonstrated high diagnostic performance for detecting active HEV infection. This assay represents a viable alternative to RT-PCR for large-scale surveillance, particularly in resource-constrained settings, and may contribute to improved control of zoonotic HEV transmission.
- 19 February 2026
- Published by AccScience Publishing in Microbes & Immunity
- Vol. 3 (3) , 025470126
Open AccessEvaluation of chicken egg yolk immunoglobulin Y-based ELISA for serodiagnosis of hepatitis E virus in swine
Hepatitis E virus (HEV) is a major zoonotic pathogen, and pigs are an important reservoir. Serology for anti-HEV antibodies poorly reflects active infection, while reverse transcription polymerase chain reaction (RT-PCR) is sensitive but not always feasible for large-scale surveillance. This study aimed to develop an immunoglobulin Y (IgY)-based sandwich enzyme-linked immunosorbent assay (ELISA) for direct detection of HEV antigen (HEV-Ag). An antigenic epitope from the HEV open reading frame 2 capsid protein was identified in silico, synthesized, and used to immunize laying hens; IgY was purified from egg yolk and characterized by sodium dodecyl sulfate–polyacrylamide gel electrophoresis. An in-house sandwich ELISA was subsequently developed using the anti-HEV IgY antibodies. Its diagnostic performance was evaluated against RT-PCR using 350 field-collected pig serum samples and compared with commercial anti-HEV IgG and IgM ELISA kits. Immunization yielded high-purity IgY antibodies, with antigen-specific antibody kinetics showing a significant increase in specific immunoreactivity from week 4 onward and peak sensitivity at weeks 6–8 post-immunization. The anti-HEV IgY showed high specificity, with no cross-reactivity observed against a heterologous antigen (HBsAg). The optimized sandwich ELISA detected HEV-Ag with a sensitivity of 95.4% and a specificity of 100% relative to RT-PCR. The prevalence of HEV-Ag was 17.7% (62/350), which was not significantly different from RT-PCR (18.6%, 65/350; p=0.250). In contrast, commercial IgG and IgM ELISAs showed high seroprevalence rates (34.9% and 28.3%, respectively) but exhibited significant discrepancies with RT-PCR (p<0.0001), indicating limited utility for identifying active infection. Overall, the developed IgY-based sandwich ELISA demonstrated high diagnostic performance for detecting active HEV infection. This assay represents a viable alternative to RT-PCR for large-scale surveillance, particularly in resource-constrained settings, and may contribute to improved control of zoonotic HEV transmission.