Chenyu Wang
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Chenyu Wang has a total of 5 co-authors. Here are the top co-authors by number of shared publications:
- Chuyuan Zhou
State Key Laboratory of Water Pollution Control and Green Resource Recycling, Key Laboratory of Aquatic Ecosystem Health in the Middle and Lower Reaches of Yangtze River, Ministry of Ecology and Environment, School of Environment, Nanjing University, Nanjing 210023, China
1 shared publication - Jingyun Yin
State Key Laboratory of Water Pollution Control and Green Resource Recycling, Key Laboratory of Aquatic Ecosystem Health in the Middle and Lower Reaches of Yangtze River of Ministry of Ecology and Environment, School of Environment, Nanjing University, Nanjing 210023, China
1 shared publication - Liuyan Yang
State Key Laboratory of Water Pollution Control and Green Resource Recycling, Key Laboratory of Aquatic Ecosystem Health in the Middle and Lower Reaches of Yangtze River of Ministry of Ecology and Environment, School of Environment, Nanjing University, Nanjing 210023, China
1 shared publication - Mengmeng Wang
State Key Laboratory of Water Pollution Control and Green Resource Recycling, Key Laboratory of Aquatic Ecosystem Health in the Middle and Lower Reaches of Yangtze River of Ministry of Ecology and Environment, School of Environment, Nanjing University, Nanjing 210023, China
1 shared publication - Xiaohua Song
State Key Laboratory of Water Pollution Control and Green Resource Recycling, Key Laboratory of Aquatic Ecosystem Health in the Middle and Lower Reaches of Yangtze River, Ministry of Ecology and Environment, School of Environment, Nanjing University, Nanjing 210023, China
1 shared publication
Latest Publications
- Open Access
High-Purity Isolation of Polyphosphate-Rich Stabilisomes Defines Their Conserved Chemical Architecture in Thermophilic Cyanobacteria
Thermophilic cyanobacteria are key models for thermotolerance and a promising source of thermophilic bioresources. Yet the subcellular basis of their stress resilience remains poorly resolved. Here, we focus on intracellular polyphosphate (polyP)-rich granules, termed “stabilisomes,” which have been implicated in stress adaptation. The lack of a high-purity, structure-preserving isolation method has been a major technical bottleneck hindering the elucidation of this resilience mechanism. This study describes a robust, structure-preserving purification strategy, boosting the granule-to-protein yield by over 10,000-fold compared with conventional methods. The specificity and structural integrity of this method are supported by the specific enrichment of complex proteomic (937 proteins) and metabolomic (1076 metabolites) signatures. Building on this, subsequent quantitative analysis across cyanobacteria at 7 hot spring sampling sites revealed a conserved core chemical composition dominated by polyphosphate (~21–36%), proteins (~10–20%), amino acids (~7–18%), and lipid components (~12–21%). The variability in abundance across species suggests a dynamic adjustment of these stabilizing components consistent with specific micro-environmental conditions. This work provides a robust bioseparation platform for prokaryotic organelles, offering a critical tool for investigating cyanobacterial resilience and developing novel biomaterials.
Open AccessHigh-Purity Isolation of Polyphosphate-Rich Stabilisomes Defines Their Conserved Chemical Architecture in Thermophilic Cyanobacteria
Thermophilic cyanobacteria are key models for thermotolerance and a promising source of thermophilic bioresources. Yet the subcellular basis of their stress resilience remains poorly resolved. Here, we focus on intracellular polyphosphate (polyP)-rich granules, termed “stabilisomes,” which have been implicated in stress adaptation. The lack of a high-purity, structure-preserving isolation method has been a major technical bottleneck hindering the elucidation of this resilience mechanism. This study describes a robust, structure-preserving purification strategy, boosting the granule-to-protein yield by over 10,000-fold compared with conventional methods. The specificity and structural integrity of this method are supported by the specific enrichment of complex proteomic (937 proteins) and metabolomic (1076 metabolites) signatures. Building on this, subsequent quantitative analysis across cyanobacteria at 7 hot spring sampling sites revealed a conserved core chemical composition dominated by polyphosphate (~21–36%), proteins (~10–20%), amino acids (~7–18%), and lipid components (~12–21%). The variability in abundance across species suggests a dynamic adjustment of these stabilizing components consistent with specific micro-environmental conditions. This work provides a robust bioseparation platform for prokaryotic organelles, offering a critical tool for investigating cyanobacterial resilience and developing novel biomaterials.