Jingyang Dong
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Jingyang Dong has a total of 20 co-authors. Here are the top co-authors by number of shared publications:
- Mengxun Li
College of Animal Science and Technology, Shihezi University, Shihezi 832003, China
2 shared publications - Tao Huang
College of Animal Science and Technology, Shihezi University, Shihezi 832003, China
2 shared publications - Yun Feng
College of Animal Science and Technology, Shihezi University, Shihezi 832003, China
2 shared publications - Binbin Fu
College of Animal Science and Technology, Shihezi University, Shihezi 832003, China
1 shared publication - Dan Wang
The Eighth Division Livestock and Fisheries Development Center, Shihezi 832000, China
1 shared publication - Fahui Yang
College of Animal Science and Technology, Shihezi University, Shihezi 832000, China
1 shared publication
Latest Publications
- Open Access
lncRNA-5583 Modulates Porcine Granulosa Cell Proliferation Through a miR-193a-5p/ACVR1-Associated Mechanism
Ovarian follicular development is essential for reproductive performance in sows, with granulosa cells playing a central role in follicular growth and function. This study investigated the function and regulatory mechanism of lncRNA-ALDBSSCT0000005583 (lncRNA-5583), previously identified as differentially expressed in M2-stage follicles (5.0–6.9 mm in diameter) between Meishan and Duroc sows. Overexpression and loss-of-function experiments were performed in porcine granulosa cells, and candidate microRNA interactions and downstream targets were examined using bioinformatic prediction, dual-luciferase reporter assays, and molecular and functional analyses. Porcine granulosa cell experiments were performed using three independent pooled granulosa-cell isolations (n = 3). LncRNA-5583 was identified as a 588-nucleotide non-coding RNA. Its overexpression promoted granulosa cell proliferation and reduced apoptosis, accompanied by corresponding changes in PCNA, BAX, Bcl-2, and the BAX/Bcl-2 ratio; miR-193a-5p overexpression attenuated these effects. LncRNA-5583 interacted with miR-193a-5p in a sequence-dependent manner, and miR-193a-5p directly targeted ACVR1. Inhibition of miR-193a-5p increased ACVR1 expression and was accompanied by increased Smad3 phosphorylation, whereas lncRNA-5583 knockdown decreased both ACVR1 expression and Smad3 phosphorylation. These findings support the involvement of the lncRNA-5583/miR-193a-5p/ACVR1 regulatory axis in porcine granulosa cell proliferation.
Open AccesslncRNA-5583 Modulates Porcine Granulosa Cell Proliferation Through a miR-193a-5p/ACVR1-Associated Mechanism
Ovarian follicular development is essential for reproductive performance in sows, with granulosa cells playing a central role in follicular growth and function. This study investigated the function and regulatory mechanism of lncRNA-ALDBSSCT0000005583 (lncRNA-5583), previously identified as differentially expressed in M2-stage follicles (5.0–6.9 mm in diameter) between Meishan and Duroc sows. Overexpression and loss-of-function experiments were performed in porcine granulosa cells, and candidate microRNA interactions and downstream targets were examined using bioinformatic prediction, dual-luciferase reporter assays, and molecular and functional analyses. Porcine granulosa cell experiments were performed using three independent pooled granulosa-cell isolations (n = 3). LncRNA-5583 was identified as a 588-nucleotide non-coding RNA. Its overexpression promoted granulosa cell proliferation and reduced apoptosis, accompanied by corresponding changes in PCNA, BAX, Bcl-2, and the BAX/Bcl-2 ratio; miR-193a-5p overexpression attenuated these effects. LncRNA-5583 interacted with miR-193a-5p in a sequence-dependent manner, and miR-193a-5p directly targeted ACVR1. Inhibition of miR-193a-5p increased ACVR1 expression and was accompanied by increased Smad3 phosphorylation, whereas lncRNA-5583 knockdown decreased both ACVR1 expression and Smad3 phosphorylation. These findings support the involvement of the lncRNA-5583/miR-193a-5p/ACVR1 regulatory axis in porcine granulosa cell proliferation.
- Open Access
Genetic Diversity and Selection Signal Analysis of Xinjiang Black Pig Based on Whole Genome Resequencing
Background: The Xinjiang Black pig is an excellent breed developed by the Xinjiang Production and Construction Corps in the 1990s; however, it has been endangered by the impact of commercial breeds. Methods: Whole genomes of 224 individuals from the Xinjiang Black pig conservation population were resequenced. Results: Genetic structure and diversity analyses revealed that Xinjiang Black pigs underwent severe inbreeding and were genetically closely linked to Landrace pigs. The genetic diversity of the F2 generation was well preserved in the existing breeding scheme. A total of 686 significant selection regions and 406 candidate genes were identified using FST and θπ complementary methods, with Xinjiang Black pigs, Min pigs, and Laiwu pigs as ancestral populations, and F2. Based on Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, and quantitative trait loci annotations, potential germplasm candidate genes were identified. Among these, SOX5, HMG20A, and NEDD4 are associated with fat deposition; SPRY1, MNS1, DMXL2, and ALB are closely associated with male reproductive ability; ARPP19 and TLN2 are strongly associated with oestrous cycle regulation and oocyte maturation; and SLC4A4 and SLC12A1 are extremely important for osmotic regulation and foetal survival. Conclusions: These findings deepen our understanding of the genetic mechanisms of artificial selection in Xinjiang Black pigs and provide a theoretical basis for subsequent breeding and genetic research on this breed.
Open AccessGenetic Diversity and Selection Signal Analysis of Xinjiang Black Pig Based on Whole Genome Resequencing
Background: The Xinjiang Black pig is an excellent breed developed by the Xinjiang Production and Construction Corps in the 1990s; however, it has been endangered by the impact of commercial breeds. Methods: Whole genomes of 224 individuals from the Xinjiang Black pig conservation population were resequenced. Results: Genetic structure and diversity analyses revealed that Xinjiang Black pigs underwent severe inbreeding and were genetically closely linked to Landrace pigs. The genetic diversity of the F2 generation was well preserved in the existing breeding scheme. A total of 686 significant selection regions and 406 candidate genes were identified using FST and θπ complementary methods, with Xinjiang Black pigs, Min pigs, and Laiwu pigs as ancestral populations, and F2. Based on Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, and quantitative trait loci annotations, potential germplasm candidate genes were identified. Among these, SOX5, HMG20A, and NEDD4 are associated with fat deposition; SPRY1, MNS1, DMXL2, and ALB are closely associated with male reproductive ability; ARPP19 and TLN2 are strongly associated with oestrous cycle regulation and oocyte maturation; and SLC4A4 and SLC12A1 are extremely important for osmotic regulation and foetal survival. Conclusions: These findings deepen our understanding of the genetic mechanisms of artificial selection in Xinjiang Black pigs and provide a theoretical basis for subsequent breeding and genetic research on this breed.