Sofia M. Gulova
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Sofia M. Gulova has a total of 6 co-authors. Here are the top co-authors by number of shared publications:
- Alexander N. Shumeev
Division of Immunobiology and Biomedicine, Sirius University of Science and Technology, 354349 Sirius, Russia
1 shared publication - Alexandra V. Pavlova
Division of Immunobiology and Biomedicine, Sirius University of Science and Technology, 354349 Sirius, Russia
1 shared publication - Alina E. Ershova
Division of Immunobiology and Biomedicine, Sirius University of Science and Technology, 354349 Sirius, Russia
1 shared publication - Daria A. Bogdanova
Division of Immunobiology and Biomedicine, Sirius University of Science and Technology, 354349 Sirius, Russia
1 shared publication - Irina V. Astrakhantseva
Department of Immunobiology and Biomedicine, Sirius University of Science and Technology, 354349 Sirius, Krasnodarsky Krai, Russia
1 shared publication - Sofiia N. Rizatdinova
Division of Immunobiology and Biomedicine, Sirius University of Science and Technology, 354349 Sirius, Russia
1 shared publication
Latest Publications
- Open Access
Development of a Multiplexed Fluorescent Pseudovirus Neutralization Test for Simultaneous Assessment of Immunity to Three SARS-CoV-2 Variants
In the post-pandemic era, monitoring adaptive immunity of the population to emerging SARS-CoV-2 variants remains an important public health priority. To address this need, we developed a test that can simultaneously assess the neutralization ability of three SARS-CoV-2 variants. A panel of lentiviral pseudoviruses, each bearing the S-protein of different SARS-CoV-2 variants (Wuhan-Hu-1, BA.1, and XBB.1.5) and expressing a unique fluorescent protein (Clover, mRhubarb713, or mRuby3) was generated and used to transduce hACE2-overexpressing cells. The percentage of infected target cells for each variant was quantified via flow cytometry. Co-infection led to a minor reduction in the percentage of infected cells compared to mono-infection controls, confirming the robustness of the assay. We then applied the test to the analysis of human sera samples, which were collected in the Sirius Federal Territory (Russian Federation) and revealed the following: (1) sera collected in 2021 neutralized the Wuhan-Hu-1 variant and demonstrated cross-specificity to the BA.1 variant, but not to the XBB.1.5 variant; (2) sera collected after the Omicron emergence point neutralized Wuhan-Hu-1 and BA.1, and possessed a weak ability to neutralize the XBB.1.5. This assay provides a valuable tool for efficient profiling of humoral immunity and monitoring its development in response to ongoing viral diversity.
Open AccessDevelopment of a Multiplexed Fluorescent Pseudovirus Neutralization Test for Simultaneous Assessment of Immunity to Three SARS-CoV-2 Variants
In the post-pandemic era, monitoring adaptive immunity of the population to emerging SARS-CoV-2 variants remains an important public health priority. To address this need, we developed a test that can simultaneously assess the neutralization ability of three SARS-CoV-2 variants. A panel of lentiviral pseudoviruses, each bearing the S-protein of different SARS-CoV-2 variants (Wuhan-Hu-1, BA.1, and XBB.1.5) and expressing a unique fluorescent protein (Clover, mRhubarb713, or mRuby3) was generated and used to transduce hACE2-overexpressing cells. The percentage of infected target cells for each variant was quantified via flow cytometry. Co-infection led to a minor reduction in the percentage of infected cells compared to mono-infection controls, confirming the robustness of the assay. We then applied the test to the analysis of human sera samples, which were collected in the Sirius Federal Territory (Russian Federation) and revealed the following: (1) sera collected in 2021 neutralized the Wuhan-Hu-1 variant and demonstrated cross-specificity to the BA.1 variant, but not to the XBB.1.5 variant; (2) sera collected after the Omicron emergence point neutralized Wuhan-Hu-1 and BA.1, and possessed a weak ability to neutralize the XBB.1.5. This assay provides a valuable tool for efficient profiling of humoral immunity and monitoring its development in response to ongoing viral diversity.